Immunostimulants, Vaccines, and Environmental Stressors in Aquaculture: NBT Assays to show Neutrophil Activity by these Immunomodulators

While neutrophil activity assays have been used in human and veterinary medicine for diagnosis of bloodneutrophil abnormalities, they are rarely used because of difficulties of handling nitro blue tetrazolium(NBT) solutions, drawing and preparation of blood samples and subjectivities in visual readi...

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Autor principal: P. Anderson, Douglas
Formato: Artículo
Lenguaje:inglés
Publicado: Facultad de Ciencias Biologicas 2019
Acceso en línea:https://nutricionacuicola.uanl.mx/index.php/acu/article/view/203
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author P. Anderson, Douglas
author_facet P. Anderson, Douglas
author_sort P. Anderson, Douglas
collection Artículos de Revistas UANL
description While neutrophil activity assays have been used in human and veterinary medicine for diagnosis of bloodneutrophil abnormalities, they are rarely used because of difficulties of handling nitro blue tetrazolium(NBT) solutions, drawing and preparation of blood samples and subjectivities in visual readings. We havedeveloped modifications for a simplified NBT assay that can be used in the diagnosis and tracing theeffectiveness of immunostimulants, vaccines and environmental stressors of fish. While training andpractice for biologists and technicians is needed, the NBT assays can yield good results when applied tofish.
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physical Avances en Nutrición Acuicola; 2004: Memorias del Septimo Simposium Internacional de Nutrición Acuícola
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spelling nutrucionacuicola-article-2032019-11-05T23:48:44Z Immunostimulants, Vaccines, and Environmental Stressors in Aquaculture: NBT Assays to show Neutrophil Activity by these Immunomodulators Immunostimulants, Vaccines, and Environmental Stressors in Aquaculture: NBT Assays to show Neutrophil Activity by these Immunomodulators P. Anderson, Douglas While neutrophil activity assays have been used in human and veterinary medicine for diagnosis of bloodneutrophil abnormalities, they are rarely used because of difficulties of handling nitro blue tetrazolium(NBT) solutions, drawing and preparation of blood samples and subjectivities in visual readings. We havedeveloped modifications for a simplified NBT assay that can be used in the diagnosis and tracing theeffectiveness of immunostimulants, vaccines and environmental stressors of fish. While training andpractice for biologists and technicians is needed, the NBT assays can yield good results when applied tofish. While neutrophil activity assays have been used in human and veterinary medicine for diagnosis of bloodneutrophil abnormalities, they are rarely used because of difficulties of handling nitro blue tetrazolium(NBT) solutions, drawing and preparation of blood samples and subjectivities in visual readings. We havedeveloped modifications for a simplified NBT assay that can be used in the diagnosis and tracing theeffectiveness of immunostimulants, vaccines and environmental stressors of fish. While training andpractice for biologists and technicians is needed, the NBT assays can yield good results when applied tofish. Facultad de Ciencias Biologicas 2019-11-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artículo revisado por pares application/pdf https://nutricionacuicola.uanl.mx/index.php/acu/article/view/203 Avances en Nutrición Acuicola; 2004: Memorias del Septimo Simposium Internacional de Nutrición Acuícola eng https://nutricionacuicola.uanl.mx/index.php/acu/article/view/203/201 Derechos de autor 2019 Douglas P. Anderson
spellingShingle P. Anderson, Douglas
Immunostimulants, Vaccines, and Environmental Stressors in Aquaculture: NBT Assays to show Neutrophil Activity by these Immunomodulators
thumbnail https://rediab.uanl.mx/themes/sandal5/images/article.gif
title Immunostimulants, Vaccines, and Environmental Stressors in Aquaculture: NBT Assays to show Neutrophil Activity by these Immunomodulators
title_alt Immunostimulants, Vaccines, and Environmental Stressors in Aquaculture: NBT Assays to show Neutrophil Activity by these Immunomodulators
title_full Immunostimulants, Vaccines, and Environmental Stressors in Aquaculture: NBT Assays to show Neutrophil Activity by these Immunomodulators
title_fullStr Immunostimulants, Vaccines, and Environmental Stressors in Aquaculture: NBT Assays to show Neutrophil Activity by these Immunomodulators
title_full_unstemmed Immunostimulants, Vaccines, and Environmental Stressors in Aquaculture: NBT Assays to show Neutrophil Activity by these Immunomodulators
title_short Immunostimulants, Vaccines, and Environmental Stressors in Aquaculture: NBT Assays to show Neutrophil Activity by these Immunomodulators
title_sort immunostimulants vaccines and environmental stressors in aquaculture nbt assays to show neutrophil activity by these immunomodulators
url https://nutricionacuicola.uanl.mx/index.php/acu/article/view/203
work_keys_str_mv AT pandersondouglas immunostimulantsvaccinesandenvironmentalstressorsinaquaculturenbtassaystoshowneutrophilactivitybytheseimmunomodulators