Immunostimulants, Vaccines, and Environmental Stressors in Aquaculture: NBT Assays to show Neutrophil Activity by these Immunomodulators

While neutrophil activity assays have been used in human and veterinary medicine for diagnosis of bloodneutrophil abnormalities, they are rarely used because of difficulties of handling nitro blue tetrazolium(NBT) solutions, drawing and preparation of blood samples and subjectivities in visual readi...

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Detalles Bibliográficos
Autor principal: P. Anderson, Douglas
Formato: Artículo
Lenguaje:inglés
Publicado: Facultad de Ciencias Biologicas 2019
Acceso en línea:https://nutricionacuicola.uanl.mx/index.php/acu/article/view/203
Descripción
Sumario:While neutrophil activity assays have been used in human and veterinary medicine for diagnosis of bloodneutrophil abnormalities, they are rarely used because of difficulties of handling nitro blue tetrazolium(NBT) solutions, drawing and preparation of blood samples and subjectivities in visual readings. We havedeveloped modifications for a simplified NBT assay that can be used in the diagnosis and tracing theeffectiveness of immunostimulants, vaccines and environmental stressors of fish. While training andpractice for biologists and technicians is needed, the NBT assays can yield good results when applied tofish.
Descripción Física:Avances en Nutrición Acuicola; 2004: Memorias del Septimo Simposium Internacional de Nutrición Acuícola