| Sumario: | Vibrio disease is being described as a major bacterial disease obviously known as penaeid bacterial septicaemia,penaeid Vibriosis, luminescent Vibriosis or red leg diseases. Signs of Vibrio disease include lethargy, tissue andappendage necrosis, slow growth, slow larval metamorphosis, body malformation, bioluminescence in shrimpparticularly produced in floc systems, muscle opacity, melanization, empty midgut and anorexia. In Asia, V.alginolyticus and V. harveyi were considered as the most significant pathogens in the grow-out ponds of giant blacktiger shrimp Penaeus monodon. Survival and pathogenicity of Vibrio was associated with the biofilm formation andquorum sensing. Therefore, disruption of biofilm formation and/or quorum sensing would be an effective managementstrategy in aquatic systems instead of killing the pathogens which obviously leads to the development of resistantstrains. Biosurfactants are surface active smart biomolecules showed strong antibiofilm activity against Vibriopathogens. In this report, biofilm producing Vibrio pathogens include V. harveyi VB1, V. alginolyticus VB2, V.vulnificus VB3, V. fischeri VB4, V. parahaemolyticus VB5 and Photobacterium damselae VB6 were isolated fromthe moribund shrimp samples collected from farms located southeast coast of India. Based on their surface-activeproperties, we hypothesized that biosurfactants could disrupt biofilms of Vibrio pathogens. To test the hypothesis, weexamined the effects of the lipopeptides extracted from marine bacteria MSI-A 07 and MSI-A 08, on the biofilmformingcapacity of biofilm infection causing pathogenic Vibrio spp. (V. harveyi VB1, V. alginolyticus VB2, V.vulnificus VB3, V. fischeri VB4, V. parahaemolyticus VB5 and Photobacterium damselae VB6). The both lipopeptidebiosurfactants potentially disrupted biofilm formation under dynamic conditions. The biofilm disruption potential ofthe lipopeptide biosurfactants was consistent against all shrimp pathogens. Based on this finding, biosurfactantincorporated feed can be formulated to contain Vibrio outbreaks in shrimp aquaculture.
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