| Sumario: | Processed animal protein ingredients are valuable ingredients for aquaculture feed formulations. However, the variability of the chemical composition of different batches of these ingredients and the relatively low digestibility of some of the nutrients (i.e. amino acids, phosphorus) occasionally represent significant limitations for these ingredients at high levels in the diet of some species. Research efforts carried out at the University of Guelph explored the potential of simple and potentially costeffective processing techniques to improve the digestibility and nutritive value of these ingredients. Processing aimed at improving digestibility of phosphorus showed that incubation with different organic acids and a chelating agent and fine grinding significantly improved in vitro bio-availability of bone phosphorus of high ash poultry by-products meal. However, this processing offered no advantage in terms of in vivo digestibility of phosphorus to rainbow trout, a species with an acid stomach. The technique may be useful for species lacking an acid stomach (e.g. carps, shrimp) but this hypothesis has not been verified. In another series of research efforts, incubation of feather meals with protease and a reducing agent, aiming to disrupt residual disulphide bonds and cross-linkage of keratin, significantly improved in vivo digestibility of protein and amino acids and bio-availability of arginine of this ingredient to rainbow trout. The results illustrate the potential of simple processing techniques, based on sound chemical principles, to improve the bio-availability of nutrients of processed animal protein ingredients. However, careful animal assays need to be carried out to confirm the usefulness of these techniques in different species.
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