In vivo evaluation of the antitumor and immunogenic properties of silver and sodium dichloroacetate combination against melanoma
Our main focus was to evaluate the efficacy of silver and sodium dichloroacetate as dual-function agents to be used in melanoma, treatment. This strategy is designed to increase the activity of these two compounds that affect DNA integrity and the mitochondria, at different levels. Furthermore, we e...
| Autores principales: | , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
John Wiley & Sons
2020
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| Acceso en línea: | http://eprints.uanl.mx/29692/1/29692.pdf |
| _version_ | 1838551020513263616 |
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| author | Torres Cavazos, Zaida Franco Molina, Moisés Armides Santana Krimskaya, Silvia Elena Rodríguez Padilla, Cristina Kawas Garza, Jorge Ramsy Hernández Vidal, Gustavo Moreno Degollado, Gustavo Zamora Ávila, Diana Elisa |
| author_facet | Torres Cavazos, Zaida Franco Molina, Moisés Armides Santana Krimskaya, Silvia Elena Rodríguez Padilla, Cristina Kawas Garza, Jorge Ramsy Hernández Vidal, Gustavo Moreno Degollado, Gustavo Zamora Ávila, Diana Elisa |
| author_sort | Torres Cavazos, Zaida |
| collection | Repositorio Institucional |
| description | Our main focus was to evaluate the efficacy of silver and sodium dichloroacetate as dual-function agents to be used in melanoma, treatment. This strategy is designed to increase the activity of these two compounds that affect DNA integrity and the mitochondria, at different levels. Furthermore, we evaluated if the cell death mechanism induced by our treatments was immunogenic cell death. To evaluate antitumor efficacy, we assessed tumor volume and production of tumor necrosis factor-α, nuclear factor κ B (both by ELISA), and nitric oxide levels (Nitrate/Nitrite colorimetric assay kit); for immunogenic cell death, we evaluated the release of danger-associated molecular patterns using immunohistochemistry and flow cytometry, as well as an in vivo challenge. Our results showed that the combination of colloidal silver and sodium dichloroacetate is more effective than each treatment alone and that the antitumor mechanism is not through immunogenic cell death. Furthermore, this study can broadly contribute to the development of dichloroacetate-loaded silver nanoparticles and to the design targeted pharmacological formulations to fight melanoma as well as other types of cancer. |
| format | Article |
| id | eprints-29692 |
| institution | UANL |
| language | English |
| publishDate | 2020 |
| publisher | John Wiley & Sons |
| record_format | eprints |
| spelling | eprints-296922025-07-15T16:07:26Z http://eprints.uanl.mx/29692/ In vivo evaluation of the antitumor and immunogenic properties of silver and sodium dichloroacetate combination against melanoma Torres Cavazos, Zaida Franco Molina, Moisés Armides Santana Krimskaya, Silvia Elena Rodríguez Padilla, Cristina Kawas Garza, Jorge Ramsy Hernández Vidal, Gustavo Moreno Degollado, Gustavo Zamora Ávila, Diana Elisa Our main focus was to evaluate the efficacy of silver and sodium dichloroacetate as dual-function agents to be used in melanoma, treatment. This strategy is designed to increase the activity of these two compounds that affect DNA integrity and the mitochondria, at different levels. Furthermore, we evaluated if the cell death mechanism induced by our treatments was immunogenic cell death. To evaluate antitumor efficacy, we assessed tumor volume and production of tumor necrosis factor-α, nuclear factor κ B (both by ELISA), and nitric oxide levels (Nitrate/Nitrite colorimetric assay kit); for immunogenic cell death, we evaluated the release of danger-associated molecular patterns using immunohistochemistry and flow cytometry, as well as an in vivo challenge. Our results showed that the combination of colloidal silver and sodium dichloroacetate is more effective than each treatment alone and that the antitumor mechanism is not through immunogenic cell death. Furthermore, this study can broadly contribute to the development of dichloroacetate-loaded silver nanoparticles and to the design targeted pharmacological formulations to fight melanoma as well as other types of cancer. John Wiley & Sons 2020 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/29692/1/29692.pdf http://eprints.uanl.mx/29692/1.haspreviewThumbnailVersion/29692.pdf Torres Cavazos, Zaida y Franco Molina, Moisés Armides y Santana Krimskaya, Silvia Elena y Rodríguez Padilla, Cristina y Kawas Garza, Jorge Ramsy y Hernández Vidal, Gustavo y Moreno Degollado, Gustavo y Zamora Ávila, Diana Elisa (2020) In vivo evaluation of the antitumor and immunogenic properties of silver and sodium dichloroacetate combination against melanoma. Journal of Nanomaterials, 2020. pp. 1-8. ISSN 1687-4110 doi:10.1155/2020/3741019 |
| spellingShingle | Torres Cavazos, Zaida Franco Molina, Moisés Armides Santana Krimskaya, Silvia Elena Rodríguez Padilla, Cristina Kawas Garza, Jorge Ramsy Hernández Vidal, Gustavo Moreno Degollado, Gustavo Zamora Ávila, Diana Elisa In vivo evaluation of the antitumor and immunogenic properties of silver and sodium dichloroacetate combination against melanoma |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | In vivo evaluation of the antitumor and immunogenic properties of silver and sodium dichloroacetate combination against melanoma |
| title_full | In vivo evaluation of the antitumor and immunogenic properties of silver and sodium dichloroacetate combination against melanoma |
| title_fullStr | In vivo evaluation of the antitumor and immunogenic properties of silver and sodium dichloroacetate combination against melanoma |
| title_full_unstemmed | In vivo evaluation of the antitumor and immunogenic properties of silver and sodium dichloroacetate combination against melanoma |
| title_short | In vivo evaluation of the antitumor and immunogenic properties of silver and sodium dichloroacetate combination against melanoma |
| title_sort | in vivo evaluation of the antitumor and immunogenic properties of silver and sodium dichloroacetate combination against melanoma |
| url | http://eprints.uanl.mx/29692/1/29692.pdf |
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