Biotransformation kinetics of pharmaceutical and industrial micropollutants in groundwaters by a laccase cocktail from Pycnoporus sanguineus CS43 fungi

In this work, the biocatalytic ability of laccases from filtered culture supernatant of Pycnoporus sanguineus was evaluated without mediators and under mild reaction conditions. This 100 U L !1 laccase cocktail removed 50% Diclofenac, 97% b-Naphthol and 71% 2,4 Dichlorophenol within 8 h of reaction...

Descripción completa

Detalles Bibliográficos
Autores principales: Rodríguez Delgado, Melissa Marlene, Orona Návar, Carolina, García Morales, Raúl, Hernandez Luna, Carlos, Parra, Roberto, Mahlknecht, Jürgen, Ornelas Soto, Nancy
Formato: Artículo
Lenguaje:Spanish / Castilian
Publicado: Elsevier 2016
Materias:
Acceso en línea:http://eprints.uanl.mx/29656/1/29656.pdf
_version_ 1864646962018516992
author Rodríguez Delgado, Melissa Marlene
Orona Návar, Carolina
García Morales, Raúl
Hernandez Luna, Carlos
Parra, Roberto
Mahlknecht, Jürgen
Ornelas Soto, Nancy
author_facet Rodríguez Delgado, Melissa Marlene
Orona Návar, Carolina
García Morales, Raúl
Hernandez Luna, Carlos
Parra, Roberto
Mahlknecht, Jürgen
Ornelas Soto, Nancy
author_sort Rodríguez Delgado, Melissa Marlene
collection Repositorio Institucional
description In this work, the biocatalytic ability of laccases from filtered culture supernatant of Pycnoporus sanguineus was evaluated without mediators and under mild reaction conditions. This 100 U L !1 laccase cocktail removed 50% Diclofenac, 97% b-Naphthol and 71% 2,4 Dichlorophenol within 8 h of reaction and 78% for 5,7-Diiodo-8-hydroxyquinoline within 3.5 h; at initial concentrations of 10 mg L!1 and at 25 "C. Furthermore, this enzyme cocktail also removed in excess of 53% all tested compounds in a real groundwater sample from northwestern Mexico. In comparison with purified laccases, the use of cocktail offers operational advantages since additional purification steps can be avoided.
format Article
id eprints-29656
institution UANL
language Spanish / Castilian
publishDate 2016
publisher Elsevier
record_format eprints
spelling eprints-296562026-05-06T15:01:08Z http://eprints.uanl.mx/29656/ Biotransformation kinetics of pharmaceutical and industrial micropollutants in groundwaters by a laccase cocktail from Pycnoporus sanguineus CS43 fungi Rodríguez Delgado, Melissa Marlene Orona Návar, Carolina García Morales, Raúl Hernandez Luna, Carlos Parra, Roberto Mahlknecht, Jürgen Ornelas Soto, Nancy Q Ciencias en General In this work, the biocatalytic ability of laccases from filtered culture supernatant of Pycnoporus sanguineus was evaluated without mediators and under mild reaction conditions. This 100 U L !1 laccase cocktail removed 50% Diclofenac, 97% b-Naphthol and 71% 2,4 Dichlorophenol within 8 h of reaction and 78% for 5,7-Diiodo-8-hydroxyquinoline within 3.5 h; at initial concentrations of 10 mg L!1 and at 25 "C. Furthermore, this enzyme cocktail also removed in excess of 53% all tested compounds in a real groundwater sample from northwestern Mexico. In comparison with purified laccases, the use of cocktail offers operational advantages since additional purification steps can be avoided. Elsevier 2016 Article PeerReviewed text es cc_by_nc_nd http://eprints.uanl.mx/29656/1/29656.pdf http://eprints.uanl.mx/29656/1.haspreviewThumbnailVersion/29656.pdf Rodríguez Delgado, Melissa Marlene y Orona Návar, Carolina y García Morales, Raúl y Hernandez Luna, Carlos y Parra, Roberto y Mahlknecht, Jürgen y Ornelas Soto, Nancy (2016) Biotransformation kinetics of pharmaceutical and industrial micropollutants in groundwaters by a laccase cocktail from Pycnoporus sanguineus CS43 fungi. International Biodeterioration & Biodegradation, 108. pp. 34-41. ISSN 0964-8305 doi:10.1016/j.ibiod.2015.12.003
spellingShingle Q Ciencias en General
Rodríguez Delgado, Melissa Marlene
Orona Návar, Carolina
García Morales, Raúl
Hernandez Luna, Carlos
Parra, Roberto
Mahlknecht, Jürgen
Ornelas Soto, Nancy
Biotransformation kinetics of pharmaceutical and industrial micropollutants in groundwaters by a laccase cocktail from Pycnoporus sanguineus CS43 fungi
thumbnail https://rediab.uanl.mx/themes/sandal5/images/online.png
title Biotransformation kinetics of pharmaceutical and industrial micropollutants in groundwaters by a laccase cocktail from Pycnoporus sanguineus CS43 fungi
title_full Biotransformation kinetics of pharmaceutical and industrial micropollutants in groundwaters by a laccase cocktail from Pycnoporus sanguineus CS43 fungi
title_fullStr Biotransformation kinetics of pharmaceutical and industrial micropollutants in groundwaters by a laccase cocktail from Pycnoporus sanguineus CS43 fungi
title_full_unstemmed Biotransformation kinetics of pharmaceutical and industrial micropollutants in groundwaters by a laccase cocktail from Pycnoporus sanguineus CS43 fungi
title_short Biotransformation kinetics of pharmaceutical and industrial micropollutants in groundwaters by a laccase cocktail from Pycnoporus sanguineus CS43 fungi
title_sort biotransformation kinetics of pharmaceutical and industrial micropollutants in groundwaters by a laccase cocktail from pycnoporus sanguineus cs43 fungi
topic Q Ciencias en General
url http://eprints.uanl.mx/29656/1/29656.pdf
work_keys_str_mv AT rodriguezdelgadomelissamarlene biotransformationkineticsofpharmaceuticalandindustrialmicropollutantsingroundwatersbyalaccasecocktailfrompycnoporussanguineuscs43fungi
AT oronanavarcarolina biotransformationkineticsofpharmaceuticalandindustrialmicropollutantsingroundwatersbyalaccasecocktailfrompycnoporussanguineuscs43fungi
AT garciamoralesraul biotransformationkineticsofpharmaceuticalandindustrialmicropollutantsingroundwatersbyalaccasecocktailfrompycnoporussanguineuscs43fungi
AT hernandezlunacarlos biotransformationkineticsofpharmaceuticalandindustrialmicropollutantsingroundwatersbyalaccasecocktailfrompycnoporussanguineuscs43fungi
AT parraroberto biotransformationkineticsofpharmaceuticalandindustrialmicropollutantsingroundwatersbyalaccasecocktailfrompycnoporussanguineuscs43fungi
AT mahlknechtjurgen biotransformationkineticsofpharmaceuticalandindustrialmicropollutantsingroundwatersbyalaccasecocktailfrompycnoporussanguineuscs43fungi
AT ornelassotonancy biotransformationkineticsofpharmaceuticalandindustrialmicropollutantsingroundwatersbyalaccasecocktailfrompycnoporussanguineuscs43fungi