| Sumario: | In this work, the biocatalytic ability of laccases from filtered culture supernatant of Pycnoporus sanguineus was evaluated without mediators and under mild reaction conditions. This 100 U L !1 laccase cocktail removed 50% Diclofenac, 97% b-Naphthol and 71% 2,4 Dichlorophenol within 8 h of reaction and 78% for 5,7-Diiodo-8-hydroxyquinoline within 3.5 h; at initial concentrations of 10 mg L!1 and at 25 "C. Furthermore, this enzyme cocktail also removed in excess of 53% all tested compounds in a real groundwater sample from northwestern Mexico. In comparison with purified laccases, the use of cocktail offers operational advantages since additional purification steps can be avoided.
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