Antimicrobial activity of gimnosperma glutinosum(spreng.) less.(asteraceae) metanol extracts against helicobacter pylori
Background: Prolonged use of antibiotics may lead to the selection of drug-resistant bacteria; as a result, efforts are being made to identify new and effective antimicrobial agents, particularly, from medicinal plants, against bacterial infections. Antimicrobial activity of Gymnosperma glutinosum a...
| Autores principales: | , , , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
African Networks on Ethnomedicines
2016
|
| Acceso en línea: | http://eprints.uanl.mx/26853/1/84.pdf |
| _version_ | 1838551009877557248 |
|---|---|
| author | Gómez Flores, Ricardo Alberto Espinosa Ramos, David Alonso Quintanilla Licea, Ramiro Barragán González, María Porfiria Tamez Guerra, Patricia Tamez Guerra, Reyes Silvestre Rodríguez Padilla, Cristina |
| author_facet | Gómez Flores, Ricardo Alberto Espinosa Ramos, David Alonso Quintanilla Licea, Ramiro Barragán González, María Porfiria Tamez Guerra, Patricia Tamez Guerra, Reyes Silvestre Rodríguez Padilla, Cristina |
| author_sort | Gómez Flores, Ricardo Alberto |
| collection | Repositorio Institucional |
| description | Background: Prolonged use of antibiotics may lead to the selection of drug-resistant bacteria; as a result, efforts are being made to identify new and effective antimicrobial agents, particularly, from medicinal plants, against bacterial infections. Antimicrobial activity of Gymnosperma glutinosum against Helicobacter pylori has not yet been reported.
Materials and methods: The antibacterial in vitro effect of Gymnosperma glutinosum methanol leaf extracts against Helicobacter pylori (ATCC 43504) was evaluated in liquid medium by the colorimetric 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) reduction assay and in solid medium by the colony forming units (CFU) method.
Results: Methanol extracts significantly (p<0.05) inhibited in vitro H. pylori growth in liquid medium from 24% to 82% at concentrations ranging from 31.25 mg/ml to 500 mg/ml, respectively, and in solid medium the extracts caused significant (p<0.05) 52% and 100% bacterial growth inhibition at concentrations of 250 µg/mL and 500 µg/mL, respectively, as compared with untreated control. Methanol vehicle did not affect H. pylori growth.
Conclusion: The observed antibacterial effect of G. glutinosum extracts may be of benefit as an adjuvant treatment of diseases caused by H. pylori. |
| format | Article |
| id | eprints-26853 |
| institution | UANL |
| language | English |
| publishDate | 2016 |
| publisher | African Networks on Ethnomedicines |
| record_format | eprints |
| spelling | eprints-268532025-07-10T16:51:02Z http://eprints.uanl.mx/26853/ Antimicrobial activity of gimnosperma glutinosum(spreng.) less.(asteraceae) metanol extracts against helicobacter pylori Gómez Flores, Ricardo Alberto Espinosa Ramos, David Alonso Quintanilla Licea, Ramiro Barragán González, María Porfiria Tamez Guerra, Patricia Tamez Guerra, Reyes Silvestre Rodríguez Padilla, Cristina Background: Prolonged use of antibiotics may lead to the selection of drug-resistant bacteria; as a result, efforts are being made to identify new and effective antimicrobial agents, particularly, from medicinal plants, against bacterial infections. Antimicrobial activity of Gymnosperma glutinosum against Helicobacter pylori has not yet been reported. Materials and methods: The antibacterial in vitro effect of Gymnosperma glutinosum methanol leaf extracts against Helicobacter pylori (ATCC 43504) was evaluated in liquid medium by the colorimetric 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) reduction assay and in solid medium by the colony forming units (CFU) method. Results: Methanol extracts significantly (p<0.05) inhibited in vitro H. pylori growth in liquid medium from 24% to 82% at concentrations ranging from 31.25 mg/ml to 500 mg/ml, respectively, and in solid medium the extracts caused significant (p<0.05) 52% and 100% bacterial growth inhibition at concentrations of 250 µg/mL and 500 µg/mL, respectively, as compared with untreated control. Methanol vehicle did not affect H. pylori growth. Conclusion: The observed antibacterial effect of G. glutinosum extracts may be of benefit as an adjuvant treatment of diseases caused by H. pylori. African Networks on Ethnomedicines 2016 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/26853/1/84.pdf http://eprints.uanl.mx/26853/1.haspreviewThumbnailVersion/84.pdf Gómez Flores, Ricardo Alberto y Espinosa Ramos, David Alonso y Quintanilla Licea, Ramiro y Barragán González, María Porfiria y Tamez Guerra, Patricia y Tamez Guerra, Reyes Silvestre y Rodríguez Padilla, Cristina (2016) Antimicrobial activity of gimnosperma glutinosum(spreng.) less.(asteraceae) metanol extracts against helicobacter pylori. African Journal of Traditional, Complementary and Alternative Medicines, 13 (4). pp. 55-59. ISSN 0189-6016 http://doi.org/10.21010/ajtcam.v13i4.9 doi:10.21010/ajtcam.v13i4.9 |
| spellingShingle | Gómez Flores, Ricardo Alberto Espinosa Ramos, David Alonso Quintanilla Licea, Ramiro Barragán González, María Porfiria Tamez Guerra, Patricia Tamez Guerra, Reyes Silvestre Rodríguez Padilla, Cristina Antimicrobial activity of gimnosperma glutinosum(spreng.) less.(asteraceae) metanol extracts against helicobacter pylori |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Antimicrobial activity of gimnosperma glutinosum(spreng.) less.(asteraceae) metanol extracts against helicobacter pylori |
| title_full | Antimicrobial activity of gimnosperma glutinosum(spreng.) less.(asteraceae) metanol extracts against helicobacter pylori |
| title_fullStr | Antimicrobial activity of gimnosperma glutinosum(spreng.) less.(asteraceae) metanol extracts against helicobacter pylori |
| title_full_unstemmed | Antimicrobial activity of gimnosperma glutinosum(spreng.) less.(asteraceae) metanol extracts against helicobacter pylori |
| title_short | Antimicrobial activity of gimnosperma glutinosum(spreng.) less.(asteraceae) metanol extracts against helicobacter pylori |
| title_sort | antimicrobial activity of gimnosperma glutinosum spreng less asteraceae metanol extracts against helicobacter pylori |
| url | http://eprints.uanl.mx/26853/1/84.pdf |
| work_keys_str_mv | AT gomezfloresricardoalberto antimicrobialactivityofgimnospermaglutinosumsprenglessasteraceaemetanolextractsagainsthelicobacterpylori AT espinosaramosdavidalonso antimicrobialactivityofgimnospermaglutinosumsprenglessasteraceaemetanolextractsagainsthelicobacterpylori AT quintanillalicearamiro antimicrobialactivityofgimnospermaglutinosumsprenglessasteraceaemetanolextractsagainsthelicobacterpylori AT barragangonzalezmariaporfiria antimicrobialactivityofgimnospermaglutinosumsprenglessasteraceaemetanolextractsagainsthelicobacterpylori AT tamezguerrapatricia antimicrobialactivityofgimnospermaglutinosumsprenglessasteraceaemetanolextractsagainsthelicobacterpylori AT tamezguerrareyessilvestre antimicrobialactivityofgimnospermaglutinosumsprenglessasteraceaemetanolextractsagainsthelicobacterpylori AT rodriguezpadillacristina antimicrobialactivityofgimnospermaglutinosumsprenglessasteraceaemetanolextractsagainsthelicobacterpylori |