Evaluation of IL-12 and CXCL-10 in patients with hepatitis C, non-alcoholic fatty liver disease and liver damage for alcohol consumption
Introduction and Objectives: To Compare serum levels of IL-12 and CXCL-10 in different etiologies of liver disease. Materials and methods: A cross-sectional and multicenter study was carried out, including subjects with alcoholism according to criteria WHO, without (OH) and with liver injury (cirrho...
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| Format: | Article |
| Language: | English |
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Ediciones Medicina y Cultura
2022
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| Online Access: | http://eprints.uanl.mx/25582/1/25582.pdf |
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| author | Hernández Santillan, M. Martínez Castillo, M. Medina Avila, Z. Lemus Peña, M Montes de Oca Ángeles, D Hernández Barragán, A. Pérez Hernández, J. L. Higuera de la Tijera, María de Fátima Santana Vargas, D. Cordero Pérez, Paula Muñoz Espinoza, Linda Córdova Gallardo, J. Kershenobich Stalnikowitz, David Gutiérrez Reyes, Gabriela |
| author_facet | Hernández Santillan, M. Martínez Castillo, M. Medina Avila, Z. Lemus Peña, M Montes de Oca Ángeles, D Hernández Barragán, A. Pérez Hernández, J. L. Higuera de la Tijera, María de Fátima Santana Vargas, D. Cordero Pérez, Paula Muñoz Espinoza, Linda Córdova Gallardo, J. Kershenobich Stalnikowitz, David Gutiérrez Reyes, Gabriela |
| author_sort | Hernández Santillan, M. |
| collection | Repositorio Institucional |
| description | Introduction and Objectives: To Compare serum levels of IL-12 and CXCL-10 in different etiologies of liver disease. Materials and methods: A cross-sectional and multicenter study was carried out, including subjects with alcoholism according to criteria WHO, without (OH) and with liver injury (cirrhosis, CiOH) and (Alcoholic Hepatitis, HA); non-alcoholic fatty liver (NAFLD) and chronic Hepatitis C (CHC), diagnosed by clinical, biochemical data. They were compared with subjects control (CT). For determination of IL-12 and CXCL-10 with Multiplex®-MERCK©. Statistical analysis by SPSS V.22 using U de Mann Whitney, p<0.05; values expressed as mean ± standard error. Results: Included 20 subjects with NAFLD, 78 CHC, 14 HA, 20 CiOH, 15 OH y 60 CT. IL-12 was found elevated in OH, HA, CHC vs. CT in OH vs. HCc y HGNA (p≤0.05). CXCL-10 was found elevated in CiOH, HA and CHC vs. CT(p≤0.050). Discussion: The IL-12 showed elevated levels in subjects with alcohol consumption and CHC vs. CT that activates other cell types involved in inflammation. CXCL-10 is induced by IFN-γ, was found elevated in CiOH, HA and CHC, exerting their biological effects through CXCR3, including activation of peripheral immune cells and apoptosis. The ratio of IL-12/CXCL-10 in OH increased 4.6 times, ratifying the participation in chronic and continual inflammatory response by alcohol consumption. Conclusions: IL-12 and CXCL-10 have an important role in alcohol-induced liver disease, confirming their contribution to inflammation, being evident CXCL-10 in advanced stages of the disease, by stimulating and favoring the migration of immune cells to the damage sites. Funding: This work was partially financed by CONACyT SALUD-2016-272579 and PAPIIT- UNAM TA200515. Declaration of interest: The authors declare no potential conflicts of interest. |
| format | Article |
| id | eprints-25582 |
| institution | UANL |
| language | English |
| publishDate | 2022 |
| publisher | Ediciones Medicina y Cultura |
| record_format | eprints |
| spelling | eprints-255822024-03-06T15:25:28Z http://eprints.uanl.mx/25582/ Evaluation of IL-12 and CXCL-10 in patients with hepatitis C, non-alcoholic fatty liver disease and liver damage for alcohol consumption Hernández Santillan, M. Martínez Castillo, M. Medina Avila, Z. Lemus Peña, M Montes de Oca Ángeles, D Hernández Barragán, A. Pérez Hernández, J. L. Higuera de la Tijera, María de Fátima Santana Vargas, D. Cordero Pérez, Paula Muñoz Espinoza, Linda Córdova Gallardo, J. Kershenobich Stalnikowitz, David Gutiérrez Reyes, Gabriela RC Medicina Interna, Psiquiatría, Neurología Introduction and Objectives: To Compare serum levels of IL-12 and CXCL-10 in different etiologies of liver disease. Materials and methods: A cross-sectional and multicenter study was carried out, including subjects with alcoholism according to criteria WHO, without (OH) and with liver injury (cirrhosis, CiOH) and (Alcoholic Hepatitis, HA); non-alcoholic fatty liver (NAFLD) and chronic Hepatitis C (CHC), diagnosed by clinical, biochemical data. They were compared with subjects control (CT). For determination of IL-12 and CXCL-10 with Multiplex®-MERCK©. Statistical analysis by SPSS V.22 using U de Mann Whitney, p<0.05; values expressed as mean ± standard error. Results: Included 20 subjects with NAFLD, 78 CHC, 14 HA, 20 CiOH, 15 OH y 60 CT. IL-12 was found elevated in OH, HA, CHC vs. CT in OH vs. HCc y HGNA (p≤0.05). CXCL-10 was found elevated in CiOH, HA and CHC vs. CT(p≤0.050). Discussion: The IL-12 showed elevated levels in subjects with alcohol consumption and CHC vs. CT that activates other cell types involved in inflammation. CXCL-10 is induced by IFN-γ, was found elevated in CiOH, HA and CHC, exerting their biological effects through CXCR3, including activation of peripheral immune cells and apoptosis. The ratio of IL-12/CXCL-10 in OH increased 4.6 times, ratifying the participation in chronic and continual inflammatory response by alcohol consumption. Conclusions: IL-12 and CXCL-10 have an important role in alcohol-induced liver disease, confirming their contribution to inflammation, being evident CXCL-10 in advanced stages of the disease, by stimulating and favoring the migration of immune cells to the damage sites. Funding: This work was partially financed by CONACyT SALUD-2016-272579 and PAPIIT- UNAM TA200515. Declaration of interest: The authors declare no potential conflicts of interest. Ediciones Medicina y Cultura 2022 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/25582/1/25582.pdf http://eprints.uanl.mx/25582/1.haspreviewThumbnailVersion/25582.pdf Hernández Santillan, M. y Martínez Castillo, M. y Medina Avila, Z. y Lemus Peña, M y Montes de Oca Ángeles, D y Hernández Barragán, A. y Pérez Hernández, J. L. y Higuera de la Tijera, María de Fátima y Santana Vargas, D. y Cordero Pérez, Paula y Muñoz Espinoza, Linda y Córdova Gallardo, J. y Kershenobich Stalnikowitz, David y Gutiérrez Reyes, Gabriela (2022) Evaluation of IL-12 and CXCL-10 in patients with hepatitis C, non-alcoholic fatty liver disease and liver damage for alcohol consumption. Annals of Hepatology, 27. p. 100865. ISSN 16652681 http://doi.org/10.1016/j.aohep.2022.100865 doi:10.1016/j.aohep.2022.100865 |
| spellingShingle | RC Medicina Interna, Psiquiatría, Neurología Hernández Santillan, M. Martínez Castillo, M. Medina Avila, Z. Lemus Peña, M Montes de Oca Ángeles, D Hernández Barragán, A. Pérez Hernández, J. L. Higuera de la Tijera, María de Fátima Santana Vargas, D. Cordero Pérez, Paula Muñoz Espinoza, Linda Córdova Gallardo, J. Kershenobich Stalnikowitz, David Gutiérrez Reyes, Gabriela Evaluation of IL-12 and CXCL-10 in patients with hepatitis C, non-alcoholic fatty liver disease and liver damage for alcohol consumption |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Evaluation of IL-12 and CXCL-10 in patients with hepatitis C, non-alcoholic fatty liver disease and liver damage for alcohol consumption |
| title_full | Evaluation of IL-12 and CXCL-10 in patients with hepatitis C, non-alcoholic fatty liver disease and liver damage for alcohol consumption |
| title_fullStr | Evaluation of IL-12 and CXCL-10 in patients with hepatitis C, non-alcoholic fatty liver disease and liver damage for alcohol consumption |
| title_full_unstemmed | Evaluation of IL-12 and CXCL-10 in patients with hepatitis C, non-alcoholic fatty liver disease and liver damage for alcohol consumption |
| title_short | Evaluation of IL-12 and CXCL-10 in patients with hepatitis C, non-alcoholic fatty liver disease and liver damage for alcohol consumption |
| title_sort | evaluation of il 12 and cxcl 10 in patients with hepatitis c non alcoholic fatty liver disease and liver damage for alcohol consumption |
| topic | RC Medicina Interna, Psiquiatría, Neurología |
| url | http://eprints.uanl.mx/25582/1/25582.pdf |
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