Pegylated interferon-α2b and ribavirin decrease claudin-1 and E-cadherin expression in HepG2 and Huh-7.5 cells
Background. Hepatitis C virus (HCV) infection usually results in long-term viremia. Entry of HCV into the he-patocyte requires claudin-1, -6, -9 and occludin. The efficacy of Pegylated interferon-α (PEG-IFN) treatment against HCV infection increased when ribavirin (RBV) was added to the therapeutic...
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| Format: | Article |
| Language: | English |
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2013
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| Online Access: | http://eprints.uanl.mx/23504/1/23504.pdf |
| _version_ | 1824416908493979648 |
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| author | Rendón Huerta, Erika P. Torres Martínez, Ana Charles Niño, Claudia Rivas Estilla, Ana María Páez, A. Fortoul, Teresa I. Montaño, Luis F. |
| author_facet | Rendón Huerta, Erika P. Torres Martínez, Ana Charles Niño, Claudia Rivas Estilla, Ana María Páez, A. Fortoul, Teresa I. Montaño, Luis F. |
| author_sort | Rendón Huerta, Erika P. |
| collection | Repositorio Institucional |
| description | Background. Hepatitis C virus (HCV) infection usually results in long-term viremia. Entry of HCV into the he-patocyte requires claudin-1, -6, -9 and occludin. The efficacy of Pegylated interferon-α (PEG-IFN) treatment against HCV infection increased when ribavirin (RBV) was added to the therapeutic scheme. Our aim was to investigate if PEG-IFN plus RBV regulate claudin expression.Material and methods. HepG2, Huh-7 and Huh-7.5 cells were treated with PEG-IFN-α2a or α2b and/or RBV at different times before obtaining the cytosolic, membrane and cytoskeletal fractions. Claudin-1, 3, 4, 6, and 9, E-cadherin and occludin expression was evaluated by Western blot analysis. Transepithelial electrical resistance (TER) was also determined.Results. Claudin-1, 3, 4, 6, E-cadherin and occludin are constitutively expressed mainly in HepG2 cell membrane. Claudin-1 and E-cadherin cell membrane expression diminished after exposure to PEGIFNα2b (50 ng) + RBV(50 μg); the maximal decrease was observed with 200 ng of PEG-IFNα2b + 200 μg of RBV. The effect was less intense with PEG-IFNα2a. The inhibition of claudin-1 and E-cadherin expression in Huh-7 and Huh-7.5 cells was only observed with 200 ng of PEG-IFNα2b + 200 μg of RBV. TER diminished marginally in the HCV containing hepatoma cells with 200 ng of PEG-IFNα2b + 200 ug of RBV. Claudin-1 mRNA expression level was not affected by the combined treatment.Conclusion. The increased therapeutic efficacy of the PEG-IFNα2b plus RBV treatment could be secondary to the inhibition of claudin-1 and E-cadherin cell membrane expression. |
| format | Article |
| id | eprints-23504 |
| institution | UANL |
| language | English |
| publishDate | 2013 |
| record_format | eprints |
| spelling | eprints-235042023-05-18T18:51:34Z http://eprints.uanl.mx/23504/ Pegylated interferon-α2b and ribavirin decrease claudin-1 and E-cadherin expression in HepG2 and Huh-7.5 cells Rendón Huerta, Erika P. Torres Martínez, Ana Charles Niño, Claudia Rivas Estilla, Ana María Páez, A. Fortoul, Teresa I. Montaño, Luis F. RC Medicina Interna, Psiquiatría, Neurología Background. Hepatitis C virus (HCV) infection usually results in long-term viremia. Entry of HCV into the he-patocyte requires claudin-1, -6, -9 and occludin. The efficacy of Pegylated interferon-α (PEG-IFN) treatment against HCV infection increased when ribavirin (RBV) was added to the therapeutic scheme. Our aim was to investigate if PEG-IFN plus RBV regulate claudin expression.Material and methods. HepG2, Huh-7 and Huh-7.5 cells were treated with PEG-IFN-α2a or α2b and/or RBV at different times before obtaining the cytosolic, membrane and cytoskeletal fractions. Claudin-1, 3, 4, 6, and 9, E-cadherin and occludin expression was evaluated by Western blot analysis. Transepithelial electrical resistance (TER) was also determined.Results. Claudin-1, 3, 4, 6, E-cadherin and occludin are constitutively expressed mainly in HepG2 cell membrane. Claudin-1 and E-cadherin cell membrane expression diminished after exposure to PEGIFNα2b (50 ng) + RBV(50 μg); the maximal decrease was observed with 200 ng of PEG-IFNα2b + 200 μg of RBV. The effect was less intense with PEG-IFNα2a. The inhibition of claudin-1 and E-cadherin expression in Huh-7 and Huh-7.5 cells was only observed with 200 ng of PEG-IFNα2b + 200 μg of RBV. TER diminished marginally in the HCV containing hepatoma cells with 200 ng of PEG-IFNα2b + 200 ug of RBV. Claudin-1 mRNA expression level was not affected by the combined treatment.Conclusion. The increased therapeutic efficacy of the PEG-IFNα2b plus RBV treatment could be secondary to the inhibition of claudin-1 and E-cadherin cell membrane expression. 2013 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/23504/1/23504.pdf http://eprints.uanl.mx/23504/1.haspreviewThumbnailVersion/23504.pdf Rendón Huerta, Erika P. y Torres Martínez, Ana y Charles Niño, Claudia y Rivas Estilla, Ana María y Páez, A. y Fortoul, Teresa I. y Montaño, Luis F. (2013) Pegylated interferon-α2b and ribavirin decrease claudin-1 and E-cadherin expression in HepG2 and Huh-7.5 cells. Annals of hepatology, 12 (4). pp. 448-457. ISSN 1665-2681 http://doi.org/10.1016/S1665-2681(19)31346-8 doi:10.1016/S1665-2681(19)31346-8 |
| spellingShingle | RC Medicina Interna, Psiquiatría, Neurología Rendón Huerta, Erika P. Torres Martínez, Ana Charles Niño, Claudia Rivas Estilla, Ana María Páez, A. Fortoul, Teresa I. Montaño, Luis F. Pegylated interferon-α2b and ribavirin decrease claudin-1 and E-cadherin expression in HepG2 and Huh-7.5 cells |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Pegylated interferon-α2b and ribavirin decrease claudin-1 and E-cadherin expression in HepG2 and Huh-7.5 cells |
| title_full | Pegylated interferon-α2b and ribavirin decrease claudin-1 and E-cadherin expression in HepG2 and Huh-7.5 cells |
| title_fullStr | Pegylated interferon-α2b and ribavirin decrease claudin-1 and E-cadherin expression in HepG2 and Huh-7.5 cells |
| title_full_unstemmed | Pegylated interferon-α2b and ribavirin decrease claudin-1 and E-cadherin expression in HepG2 and Huh-7.5 cells |
| title_short | Pegylated interferon-α2b and ribavirin decrease claudin-1 and E-cadherin expression in HepG2 and Huh-7.5 cells |
| title_sort | pegylated interferon α2b and ribavirin decrease claudin 1 and e cadherin expression in hepg2 and huh 7 5 cells |
| topic | RC Medicina Interna, Psiquiatría, Neurología |
| url | http://eprints.uanl.mx/23504/1/23504.pdf |
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