Superovulation and embryo quality with porcine follicle stimulation hormone (pFSH) in Katahdin hair sheep during breeding season

Estrus synchronization of 21 ewes was carried out using intravaginal sponges (60 mg of medroxyprogesterone acetate, Sincrogest®-Sanfer, Mexico) for 14 days. 7 ewes were randomly assigned to one of the three treatments: T1, 80 mg; T2, 120 mg; T3, 140 mg of porcine follicle stimulating (pFSH) (Foll...

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Detalles Bibliográficos
Autores principales: Sánchez Dávila, Fernando, Bernal Barragán, Hugo, Del Bosque González, Alejandro S., González Gómez, Adán, Olivares Sáenz, Emilio, Padilla Rivas, Gerardo Raymundo, Ledezma Torres, Rogelio Alejandro
Formato: Artículo
Lenguaje:inglés
Publicado: Academic Journals 2013
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Acceso en línea:http://eprints.uanl.mx/20025/1/28.pdf
Descripción
Sumario:Estrus synchronization of 21 ewes was carried out using intravaginal sponges (60 mg of medroxyprogesterone acetate, Sincrogest®-Sanfer, Mexico) for 14 days. 7 ewes were randomly assigned to one of the three treatments: T1, 80 mg; T2, 120 mg; T3, 140 mg of porcine follicle stimulating (pFSH) (Folltropin-p®-Bioniche, Canada) administered on day 12 after sponge insertion. The dose was divided in decreasing doses; second and third day representing 50 to 75% and 25 to 33% of the first day dose, respectively. At the beginning of superovulation treatment, each ewe received 5 mg of dinoprost tromethamine (1 ml of Lutalyse® Pfizer, Mexico). An effect of pFSH level was observed on the interval from sponge removal and the onset of estrus (REI) (P < 0.01), with a difference of 13.5 h between ewes from treatment 2 and 3. The number of follicles (F), number of collected embryos (CE) and transferable embryos (TE) (P < 0.05) was also different among treatments, with highest values observed in T3 (3.0 ± 0.6 for CE and 2.3 ± 0.5 for TE). Embryo recovery rate was in average 66.5%, (P > 0.05) of CL observed. Further investigation is guaranteed to evaluate the effect of pFSH doses on estrus synchronization for selected breeding seasons