Quantitative trait loci mapping of genome regions controlling permethrin resistance in the mosquito Aedes aegypti.
The mosquitoAedes aegyptiis the principal vector of dengue and yellow fever flaviviruses. Permethrin is an insecticide used to suppress Ae. aegypti adult populations but metabolic and target site resistance to pyrethroids has evolved in many locations worldwide. Quantitative trait loci (QTL) contro...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Genetics Society of America
2008
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| Online Access: | http://eprints.uanl.mx/1873/1/QTL_mapping_of_genome_regions_controlling_permethrin_resistance_in_the_mosquito_Aedes_aegypti.pdf |
| _version_ | 1824368073796222976 |
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| author | Saavedra Rodríguez, Karla Lizet Strode, Clare Flores Suárez, Adriana Elizabeth Fernández Salas, Ildefonso Ranson, Hilary Hemingway, Janet Black, William C. |
| author_facet | Saavedra Rodríguez, Karla Lizet Strode, Clare Flores Suárez, Adriana Elizabeth Fernández Salas, Ildefonso Ranson, Hilary Hemingway, Janet Black, William C. |
| author_sort | Saavedra Rodríguez, Karla Lizet |
| collection | Repositorio Institucional |
| description | The mosquitoAedes aegyptiis the principal vector of dengue and yellow fever flaviviruses. Permethrin is an
insecticide used to suppress Ae. aegypti adult populations but metabolic and target site resistance to
pyrethroids has evolved in many locations worldwide. Quantitative trait loci (QTL) controlling permethrin
survival in Ae. aegypti were mapped in an F3 advanced intercross line. Parents came from a collection of
mosquitoes from Isla Mujeres, Me´xico, that had been selected for permethrin resistance for two generations
and a reference permethrin-susceptible strain originally from New Orleans. Following a 1-hr permethrin
exposure, 439 F3 adult mosquitoes were phenotyped as knockdown resistant, knocked down/recovered, or
dead. For QTL mapping, single nucleotide polymorphisms (SNPs) were identified at 22 loci with potential
antixenobiotic activity including genes encoding cytochrome P450s (CYP), esterases (EST), or glutathione
transferases (GST) and at 12 previously mapped loci. Seven antixenobiotic genes mapped to chromosome I,
six to chromosome II, and nine to chromosome III. Two QTL of major effect were detected on chromosome
III. One corresponds with a SNP previously associated with permethrin resistance in theparasodium channel
gene and the second with theCCEunk7o esterase marker. Additional QTL but of relatively minor effect were
also found. These included two sex-linked QTL on chromosome I affecting knockdown and recovery and a
QTL affecting survival and recovery. On chromosome II, one QTL affecting survival and a second affecting
recovery were detected. The patterns confirm that mutations in the para gene cause target-site insensitivity
and are the major source of permethrin resistance but that other genes dispersed throughout the genome
contribute to recovery and survival of mosquitoes following permethrin exposure. |
| format | Article |
| id | eprints-1873 |
| institution | UANL |
| language | English |
| publishDate | 2008 |
| publisher | Genetics Society of America |
| record_format | eprints |
| spelling | eprints-18732016-02-17T16:37:56Z http://eprints.uanl.mx/1873/ Quantitative trait loci mapping of genome regions controlling permethrin resistance in the mosquito Aedes aegypti. Saavedra Rodríguez, Karla Lizet Strode, Clare Flores Suárez, Adriana Elizabeth Fernández Salas, Ildefonso Ranson, Hilary Hemingway, Janet Black, William C. The mosquitoAedes aegyptiis the principal vector of dengue and yellow fever flaviviruses. Permethrin is an insecticide used to suppress Ae. aegypti adult populations but metabolic and target site resistance to pyrethroids has evolved in many locations worldwide. Quantitative trait loci (QTL) controlling permethrin survival in Ae. aegypti were mapped in an F3 advanced intercross line. Parents came from a collection of mosquitoes from Isla Mujeres, Me´xico, that had been selected for permethrin resistance for two generations and a reference permethrin-susceptible strain originally from New Orleans. Following a 1-hr permethrin exposure, 439 F3 adult mosquitoes were phenotyped as knockdown resistant, knocked down/recovered, or dead. For QTL mapping, single nucleotide polymorphisms (SNPs) were identified at 22 loci with potential antixenobiotic activity including genes encoding cytochrome P450s (CYP), esterases (EST), or glutathione transferases (GST) and at 12 previously mapped loci. Seven antixenobiotic genes mapped to chromosome I, six to chromosome II, and nine to chromosome III. Two QTL of major effect were detected on chromosome III. One corresponds with a SNP previously associated with permethrin resistance in theparasodium channel gene and the second with theCCEunk7o esterase marker. Additional QTL but of relatively minor effect were also found. These included two sex-linked QTL on chromosome I affecting knockdown and recovery and a QTL affecting survival and recovery. On chromosome II, one QTL affecting survival and a second affecting recovery were detected. The patterns confirm that mutations in the para gene cause target-site insensitivity and are the major source of permethrin resistance but that other genes dispersed throughout the genome contribute to recovery and survival of mosquitoes following permethrin exposure. Genetics Society of America 2008 Article PeerReviewed application/pdf en http://eprints.uanl.mx/1873/1/QTL_mapping_of_genome_regions_controlling_permethrin_resistance_in_the_mosquito_Aedes_aegypti.pdf http://eprints.uanl.mx/1873/1.haspreviewThumbnailVersion/QTL_mapping_of_genome_regions_controlling_permethrin_resistance_in_the_mosquito_Aedes_aegypti.pdf Saavedra Rodríguez, Karla Lizet y Strode, Clare y Flores Suárez, Adriana Elizabeth y Fernández Salas, Ildefonso y Ranson, Hilary y Hemingway, Janet y Black, William C. (2008) Quantitative trait loci mapping of genome regions controlling permethrin resistance in the mosquito Aedes aegypti. Genetics, 180 (2). pp. 1137-1152. ISSN 0016-6731 |
| spellingShingle | Saavedra Rodríguez, Karla Lizet Strode, Clare Flores Suárez, Adriana Elizabeth Fernández Salas, Ildefonso Ranson, Hilary Hemingway, Janet Black, William C. Quantitative trait loci mapping of genome regions controlling permethrin resistance in the mosquito Aedes aegypti. |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Quantitative trait loci mapping of genome regions controlling permethrin resistance in the mosquito Aedes aegypti. |
| title_full | Quantitative trait loci mapping of genome regions controlling permethrin resistance in the mosquito Aedes aegypti. |
| title_fullStr | Quantitative trait loci mapping of genome regions controlling permethrin resistance in the mosquito Aedes aegypti. |
| title_full_unstemmed | Quantitative trait loci mapping of genome regions controlling permethrin resistance in the mosquito Aedes aegypti. |
| title_short | Quantitative trait loci mapping of genome regions controlling permethrin resistance in the mosquito Aedes aegypti. |
| title_sort | quantitative trait loci mapping of genome regions controlling permethrin resistance in the mosquito aedes aegypti |
| url | http://eprints.uanl.mx/1873/1/QTL_mapping_of_genome_regions_controlling_permethrin_resistance_in_the_mosquito_Aedes_aegypti.pdf |
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