Multicenter, International Study of MIC/MEC Distributions for Definition of Epidemiological Cutoff Values for Sporothrix Species Identified by Molecular Methods
ABSTRACT Clinical and Laboratory Standards Institute (CLSI) conditions for testing the susceptibilities of pathogenic Sporothrix species to antifungal agents are based on a collaborative study that evaluated five clinically relevant isolates of Sporothrix schenckii sensu lato and some antifungal agen...
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2017
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| Online Access: | http://eprints.uanl.mx/18282/1/573.pdf |
| _version_ | 1824415197359505408 |
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| author | Espinel Ingroff, A. Abreu, D. P. B. Almeida Paes, R. Brilhante, R. S. N. Chakrabarti, A. Chowdhary, A. Hagen, F. Córdoba, S. González González, Gloria María Govender, N. P. Guarro, Josep Johnson, E. M. Kidd, S. E. Pereira, S. A. Rodrigues, A. M. Rozental, S. Szeszs, M. W. Ballesté Alaniz, R. Bonifaz, Alexandro Bonfietti, L. X. Borba Santos, L. P. Capilla, Javier Colombo, A. L. Dolande, M. Isla, M. G. Melhem, M. S. C. Mesa Arango, A. C. Oliveira, M. M. E. Panizo, M. M. Pires de Camargo, Z. Zancope Oliveira, R. M. Meis, J. F. Turnidge, J. |
| author_facet | Espinel Ingroff, A. Abreu, D. P. B. Almeida Paes, R. Brilhante, R. S. N. Chakrabarti, A. Chowdhary, A. Hagen, F. Córdoba, S. González González, Gloria María Govender, N. P. Guarro, Josep Johnson, E. M. Kidd, S. E. Pereira, S. A. Rodrigues, A. M. Rozental, S. Szeszs, M. W. Ballesté Alaniz, R. Bonifaz, Alexandro Bonfietti, L. X. Borba Santos, L. P. Capilla, Javier Colombo, A. L. Dolande, M. Isla, M. G. Melhem, M. S. C. Mesa Arango, A. C. Oliveira, M. M. E. Panizo, M. M. Pires de Camargo, Z. Zancope Oliveira, R. M. Meis, J. F. Turnidge, J. |
| author_sort | Espinel Ingroff, A. |
| collection | Repositorio Institucional |
| description | ABSTRACT Clinical and Laboratory Standards Institute (CLSI) conditions for testing the susceptibilities of pathogenic Sporothrix species to antifungal agents are based on a collaborative study that evaluated five clinically relevant isolates of Sporothrix schenckii sensu lato and some antifungal agents. With the advent of molecular identification, there are two basic needs: to confirm the suitability of these testing conditions for all agents and Sporothrix species and to establish species-specific epidemiologic cutoff values (ECVs) or breakpoints (BPs) for the species. We collected available CLSI MICs/minimal effective concentrations (MECs) of amphotericin B, five triazoles, terbinafine, flucytosine, and caspofungin for 301 Sporothrix schenckii sensu stricto, 486 S. brasiliensis, 75S. globosa, and 13 S. mexicana molecularly identified isolates. Data were obtained in 17 independent laboratories (Australia, Europe, India, South Africa, and South and North America) using conidial inoculum suspensions and 48 to 72 h of incubation at 35°C. Sufficient and suitable data (modal MICs within 2-fold concentrations) allowed the proposal of the following ECVs for S. schenckii and S. brasiliensis, respectively: amphotericin B, 4 and 4 g/ml; itraconazole, 2 and 2 g/ml; posaconazole, 2 and 2 g/ml; and voriconazole, 64 and 32 g/ml. Ketoconazole and terbinafine ECVs for S. brasiliensis were 2 and 0.12 g/ml, respectively. Insufficient or unsuitable data precluded the calculation of ketoconazole and terbinafine (or any other antifungal agent) ECVs for S. schenckii, as well as ECVs for S. globosa and S. mexicana. These ECVs could aid the clinician in identifying potentially resistant isolates (non-wild type) less likely to respond to therapy. |
| format | Article |
| id | eprints-18282 |
| institution | UANL |
| language | English |
| publishDate | 2017 |
| publisher | American Society for Microbiology |
| record_format | eprints |
| spelling | eprints-182822024-03-05T17:54:59Z http://eprints.uanl.mx/18282/ Multicenter, International Study of MIC/MEC Distributions for Definition of Epidemiological Cutoff Values for Sporothrix Species Identified by Molecular Methods Espinel Ingroff, A. Abreu, D. P. B. Almeida Paes, R. Brilhante, R. S. N. Chakrabarti, A. Chowdhary, A. Hagen, F. Córdoba, S. González González, Gloria María Govender, N. P. Guarro, Josep Johnson, E. M. Kidd, S. E. Pereira, S. A. Rodrigues, A. M. Rozental, S. Szeszs, M. W. Ballesté Alaniz, R. Bonifaz, Alexandro Bonfietti, L. X. Borba Santos, L. P. Capilla, Javier Colombo, A. L. Dolande, M. Isla, M. G. Melhem, M. S. C. Mesa Arango, A. C. Oliveira, M. M. E. Panizo, M. M. Pires de Camargo, Z. Zancope Oliveira, R. M. Meis, J. F. Turnidge, J. ABSTRACT Clinical and Laboratory Standards Institute (CLSI) conditions for testing the susceptibilities of pathogenic Sporothrix species to antifungal agents are based on a collaborative study that evaluated five clinically relevant isolates of Sporothrix schenckii sensu lato and some antifungal agents. With the advent of molecular identification, there are two basic needs: to confirm the suitability of these testing conditions for all agents and Sporothrix species and to establish species-specific epidemiologic cutoff values (ECVs) or breakpoints (BPs) for the species. We collected available CLSI MICs/minimal effective concentrations (MECs) of amphotericin B, five triazoles, terbinafine, flucytosine, and caspofungin for 301 Sporothrix schenckii sensu stricto, 486 S. brasiliensis, 75S. globosa, and 13 S. mexicana molecularly identified isolates. Data were obtained in 17 independent laboratories (Australia, Europe, India, South Africa, and South and North America) using conidial inoculum suspensions and 48 to 72 h of incubation at 35°C. Sufficient and suitable data (modal MICs within 2-fold concentrations) allowed the proposal of the following ECVs for S. schenckii and S. brasiliensis, respectively: amphotericin B, 4 and 4 g/ml; itraconazole, 2 and 2 g/ml; posaconazole, 2 and 2 g/ml; and voriconazole, 64 and 32 g/ml. Ketoconazole and terbinafine ECVs for S. brasiliensis were 2 and 0.12 g/ml, respectively. Insufficient or unsuitable data precluded the calculation of ketoconazole and terbinafine (or any other antifungal agent) ECVs for S. schenckii, as well as ECVs for S. globosa and S. mexicana. These ECVs could aid the clinician in identifying potentially resistant isolates (non-wild type) less likely to respond to therapy. American Society for Microbiology 2017-07-20 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/18282/1/573.pdf http://eprints.uanl.mx/18282/1.haspreviewThumbnailVersion/573.pdf Espinel Ingroff, A. y Abreu, D. P. B. y Almeida Paes, R. y Brilhante, R. S. N. y Chakrabarti, A. y Chowdhary, A. y Hagen, F. y Córdoba, S. y González González, Gloria María y Govender, N. P. y Guarro, Josep y Johnson, E. M. y Kidd, S. E. y Pereira, S. A. y Rodrigues, A. M. y Rozental, S. y Szeszs, M. W. y Ballesté Alaniz, R. y Bonifaz, Alexandro y Bonfietti, L. X. y Borba Santos, L. P. y Capilla, Javier y Colombo, A. L. y Dolande, M. y Isla, M. G. y Melhem, M. S. C. y Mesa Arango, A. C. y Oliveira, M. M. E. y Panizo, M. M. y Pires de Camargo, Z. y Zancope Oliveira, R. M. y Meis, J. F. y Turnidge, J. (2017) Multicenter, International Study of MIC/MEC Distributions for Definition of Epidemiological Cutoff Values for Sporothrix Species Identified by Molecular Methods. Antimicrobial agents and chemotherapy, 61 (10). pp. 1-8. ISSN 0066-4804 http://doi.org/10.1128/AAC.01057-17 doi:10.1128/AAC.01057-17 |
| spellingShingle | Espinel Ingroff, A. Abreu, D. P. B. Almeida Paes, R. Brilhante, R. S. N. Chakrabarti, A. Chowdhary, A. Hagen, F. Córdoba, S. González González, Gloria María Govender, N. P. Guarro, Josep Johnson, E. M. Kidd, S. E. Pereira, S. A. Rodrigues, A. M. Rozental, S. Szeszs, M. W. Ballesté Alaniz, R. Bonifaz, Alexandro Bonfietti, L. X. Borba Santos, L. P. Capilla, Javier Colombo, A. L. Dolande, M. Isla, M. G. Melhem, M. S. C. Mesa Arango, A. C. Oliveira, M. M. E. Panizo, M. M. Pires de Camargo, Z. Zancope Oliveira, R. M. Meis, J. F. Turnidge, J. Multicenter, International Study of MIC/MEC Distributions for Definition of Epidemiological Cutoff Values for Sporothrix Species Identified by Molecular Methods |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Multicenter, International Study of MIC/MEC Distributions for Definition of Epidemiological Cutoff Values for Sporothrix Species Identified by Molecular Methods |
| title_full | Multicenter, International Study of MIC/MEC Distributions for Definition of Epidemiological Cutoff Values for Sporothrix Species Identified by Molecular Methods |
| title_fullStr | Multicenter, International Study of MIC/MEC Distributions for Definition of Epidemiological Cutoff Values for Sporothrix Species Identified by Molecular Methods |
| title_full_unstemmed | Multicenter, International Study of MIC/MEC Distributions for Definition of Epidemiological Cutoff Values for Sporothrix Species Identified by Molecular Methods |
| title_short | Multicenter, International Study of MIC/MEC Distributions for Definition of Epidemiological Cutoff Values for Sporothrix Species Identified by Molecular Methods |
| title_sort | multicenter international study of mic mec distributions for definition of epidemiological cutoff values for sporothrix species identified by molecular methods |
| url | http://eprints.uanl.mx/18282/1/573.pdf |
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