Cytoplasmic Localization of WT1 and Decrease of miRNA-16-1 in Nephrotic Syndrome
Nephrotic syndrome (NS) is a glomerular disease that is defined by the leakage of protein into the urine and is associated with hypoalbuminemia, hyperlipidemia, and edema. Steroid-resistant NS (SRNS) patients do not respond to treatment with corticosteroids and show decreased Wilms tumor 1 (WT1) exp...
| Autores principales: | , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | inglés |
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2017
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| Acceso en línea: | http://eprints.uanl.mx/18258/1/548.pdf |
| _version_ | 1824415188484358144 |
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| author | Zapata Benavides, Pablo Arellano Rodríguez, Mariela Bollain y Goytia de la Rosa, Juan José Franco Molina, Moisés Armides Rangel Ochoa, Gloria Azucena Avalos Díaz, Esperanza Herrera Esparza, Rafael Rodríguez Padilla, Cristina |
| author_facet | Zapata Benavides, Pablo Arellano Rodríguez, Mariela Bollain y Goytia de la Rosa, Juan José Franco Molina, Moisés Armides Rangel Ochoa, Gloria Azucena Avalos Díaz, Esperanza Herrera Esparza, Rafael Rodríguez Padilla, Cristina |
| author_sort | Zapata Benavides, Pablo |
| collection | Repositorio Institucional |
| description | Nephrotic syndrome (NS) is a glomerular disease that is defined by the leakage of protein into the urine and is associated with hypoalbuminemia, hyperlipidemia, and edema. Steroid-resistant NS (SRNS) patients do not respond to treatment with corticosteroids and show decreased Wilms tumor 1 (WT1) expression in podocytes. Downregulation of WT1 has been shown to beaffectedbycertainmicroRNAs(miRNAs).Twenty-onepatientswithidiopathicNS(68.75%wereSSNSand31.25%SRNS)and 10healthycontrolswereenrolledinthestudy.PodocytenumberandWT1locationweredeterminedbyimmunofluorescence,and the serum levels of miR-15a, miR-16-1, and miR-193a were quantified by RT-qPCR. Low expression and delocalization of WT1 proteinfromthenucleustothecytoplasmwerefoundinkidneybiopsiesofpatientswithSRNSandbothnuclearandcytoplasmic localization were found in steroid-sensitive NS (SSNS) patients. In sera from NS patients, low expression levels of miR-15a and miR-16-1 were found compared with healthy controls, but only the miR-16-1 expression levels showed statistically significant decrease ( |
| format | Article |
| id | eprints-18258 |
| institution | UANL |
| language | English |
| publishDate | 2017 |
| record_format | eprints |
| spelling | eprints-182582024-03-05T20:44:40Z http://eprints.uanl.mx/18258/ Cytoplasmic Localization of WT1 and Decrease of miRNA-16-1 in Nephrotic Syndrome Zapata Benavides, Pablo Arellano Rodríguez, Mariela Bollain y Goytia de la Rosa, Juan José Franco Molina, Moisés Armides Rangel Ochoa, Gloria Azucena Avalos Díaz, Esperanza Herrera Esparza, Rafael Rodríguez Padilla, Cristina QR Microbiología Nephrotic syndrome (NS) is a glomerular disease that is defined by the leakage of protein into the urine and is associated with hypoalbuminemia, hyperlipidemia, and edema. Steroid-resistant NS (SRNS) patients do not respond to treatment with corticosteroids and show decreased Wilms tumor 1 (WT1) expression in podocytes. Downregulation of WT1 has been shown to beaffectedbycertainmicroRNAs(miRNAs).Twenty-onepatientswithidiopathicNS(68.75%wereSSNSand31.25%SRNS)and 10healthycontrolswereenrolledinthestudy.PodocytenumberandWT1locationweredeterminedbyimmunofluorescence,and the serum levels of miR-15a, miR-16-1, and miR-193a were quantified by RT-qPCR. Low expression and delocalization of WT1 proteinfromthenucleustothecytoplasmwerefoundinkidneybiopsiesofpatientswithSRNSandbothnuclearandcytoplasmic localization were found in steroid-sensitive NS (SSNS) patients. In sera from NS patients, low expression levels of miR-15a and miR-16-1 were found compared with healthy controls, but only the miR-16-1 expression levels showed statistically significant decrease ( 2017-02-19 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/18258/1/548.pdf http://eprints.uanl.mx/18258/1.haspreviewThumbnailVersion/548.pdf Zapata Benavides, Pablo y Arellano Rodríguez, Mariela y Bollain y Goytia de la Rosa, Juan José y Franco Molina, Moisés Armides y Rangel Ochoa, Gloria Azucena y Avalos Díaz, Esperanza y Herrera Esparza, Rafael y Rodríguez Padilla, Cristina (2017) Cytoplasmic Localization of WT1 and Decrease of miRNA-16-1 in Nephrotic Syndrome. BioMed Research International, 2017. pp. 1-8. ISSN 2314-6133 http://doi.org/10.1155/2017/9531074 doi:10.1155/2017/9531074 |
| spellingShingle | QR Microbiología Zapata Benavides, Pablo Arellano Rodríguez, Mariela Bollain y Goytia de la Rosa, Juan José Franco Molina, Moisés Armides Rangel Ochoa, Gloria Azucena Avalos Díaz, Esperanza Herrera Esparza, Rafael Rodríguez Padilla, Cristina Cytoplasmic Localization of WT1 and Decrease of miRNA-16-1 in Nephrotic Syndrome |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Cytoplasmic Localization of WT1 and Decrease of miRNA-16-1 in Nephrotic Syndrome |
| title_full | Cytoplasmic Localization of WT1 and Decrease of miRNA-16-1 in Nephrotic Syndrome |
| title_fullStr | Cytoplasmic Localization of WT1 and Decrease of miRNA-16-1 in Nephrotic Syndrome |
| title_full_unstemmed | Cytoplasmic Localization of WT1 and Decrease of miRNA-16-1 in Nephrotic Syndrome |
| title_short | Cytoplasmic Localization of WT1 and Decrease of miRNA-16-1 in Nephrotic Syndrome |
| title_sort | cytoplasmic localization of wt1 and decrease of mirna 16 1 in nephrotic syndrome |
| topic | QR Microbiología |
| url | http://eprints.uanl.mx/18258/1/548.pdf |
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