Cytoplasmic Localization of WT1 and Decrease of miRNA-16-1 in Nephrotic Syndrome

Nephrotic syndrome (NS) is a glomerular disease that is defined by the leakage of protein into the urine and is associated with hypoalbuminemia, hyperlipidemia, and edema. Steroid-resistant NS (SRNS) patients do not respond to treatment with corticosteroids and show decreased Wilms tumor 1 (WT1) exp...

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Bibliographic Details
Main Authors: Zapata Benavides, Pablo, Arellano Rodríguez, Mariela, Bollain y Goytia de la Rosa, Juan José, Franco Molina, Moisés Armides, Rangel Ochoa, Gloria Azucena, Avalos Díaz, Esperanza, Herrera Esparza, Rafael, Rodríguez Padilla, Cristina
Format: Article
Language:English
Published: 2017
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Online Access:http://eprints.uanl.mx/18258/1/548.pdf
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Summary:Nephrotic syndrome (NS) is a glomerular disease that is defined by the leakage of protein into the urine and is associated with hypoalbuminemia, hyperlipidemia, and edema. Steroid-resistant NS (SRNS) patients do not respond to treatment with corticosteroids and show decreased Wilms tumor 1 (WT1) expression in podocytes. Downregulation of WT1 has been shown to beaffectedbycertainmicroRNAs(miRNAs).Twenty-onepatientswithidiopathicNS(68.75%wereSSNSand31.25%SRNS)and 10healthycontrolswereenrolledinthestudy.PodocytenumberandWT1locationweredeterminedbyimmunofluorescence,and the serum levels of miR-15a, miR-16-1, and miR-193a were quantified by RT-qPCR. Low expression and delocalization of WT1 proteinfromthenucleustothecytoplasmwerefoundinkidneybiopsiesofpatientswithSRNSandbothnuclearandcytoplasmic localization were found in steroid-sensitive NS (SSNS) patients. In sera from NS patients, low expression levels of miR-15a and miR-16-1 were found compared with healthy controls, but only the miR-16-1 expression levels showed statistically significant decrease (