“CLUPS”: A New Culture Medium for the Axenic Growth of Entamoeba histolytica
Amebiasis remains a major health problem in Mexico. Terefore, the search for better culture media and low-cost diagnostic and therapeutic tools is fundamental. We present a new culture medium for Entamoeba histolytica which allows the microbe to preserve its virulence factors and ability to induce h...
| Autores principales: | , , , , , , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
Hindawi Publishing Corporation
2018
|
| Materias: | |
| Acceso en línea: | http://eprints.uanl.mx/17149/1/253.pdf |
| _version_ | 1824414806343417856 |
|---|---|
| author | González Salazar, Francisco Meester, Irene Guzmán de la Garza, Francisco Javier De la Garza Salinas, Laura Hermila Sampayo Reyes, Adriana Garza González, Jesús Norberto Monsivais Diaz, O. Barba Dávila, B. A. Hernández García, Magda Elizabeth Vargas Villarreal, Javier |
| author_facet | González Salazar, Francisco Meester, Irene Guzmán de la Garza, Francisco Javier De la Garza Salinas, Laura Hermila Sampayo Reyes, Adriana Garza González, Jesús Norberto Monsivais Diaz, O. Barba Dávila, B. A. Hernández García, Magda Elizabeth Vargas Villarreal, Javier |
| author_sort | González Salazar, Francisco |
| collection | Repositorio Institucional |
| description | Amebiasis remains a major health problem in Mexico. Terefore, the search for better culture media and low-cost diagnostic and therapeutic tools is fundamental. We present a new culture medium for Entamoeba histolytica which allows the microbe to preserve its virulence factors and ability to induce hepatic abscesses in animal models. Te novel CLUPS medium is an improved version
of the PEHPS medium, previously designed in our laboratory. Te main diference is the substitution of raw beef liver in PEHPS by raw beef lung in the CLUPS medium. To compare the performance of three-culture media (traditional TYI-S-33, PEHPS, and CLUPS), E. histolytica trophozoites were cultured in quintuplicate, followed by the evaluation of phospholipase activity and the
induction of liver abscesses in golden hamsters. E. histolytica trophozoites grew signifcantly better in CLUPS medium than in TYIS-33. Likewise, CLUPS-cultured trophozoites produced signifcantly more phospholipases than TYI-S-33-cultured trophozoites. Finally, trophozoites grown in any of the three tested media had similar potential to induce liver abscesses |
| format | Article |
| id | eprints-17149 |
| institution | UANL |
| language | English |
| publishDate | 2018 |
| publisher | Hindawi Publishing Corporation |
| record_format | eprints |
| spelling | eprints-171492024-12-11T15:30:55Z http://eprints.uanl.mx/17149/ “CLUPS”: A New Culture Medium for the Axenic Growth of Entamoeba histolytica González Salazar, Francisco Meester, Irene Guzmán de la Garza, Francisco Javier De la Garza Salinas, Laura Hermila Sampayo Reyes, Adriana Garza González, Jesús Norberto Monsivais Diaz, O. Barba Dávila, B. A. Hernández García, Magda Elizabeth Vargas Villarreal, Javier RC Medicina Interna, Psiquiatría, Neurología Amebiasis remains a major health problem in Mexico. Terefore, the search for better culture media and low-cost diagnostic and therapeutic tools is fundamental. We present a new culture medium for Entamoeba histolytica which allows the microbe to preserve its virulence factors and ability to induce hepatic abscesses in animal models. Te novel CLUPS medium is an improved version of the PEHPS medium, previously designed in our laboratory. Te main diference is the substitution of raw beef liver in PEHPS by raw beef lung in the CLUPS medium. To compare the performance of three-culture media (traditional TYI-S-33, PEHPS, and CLUPS), E. histolytica trophozoites were cultured in quintuplicate, followed by the evaluation of phospholipase activity and the induction of liver abscesses in golden hamsters. E. histolytica trophozoites grew signifcantly better in CLUPS medium than in TYIS-33. Likewise, CLUPS-cultured trophozoites produced signifcantly more phospholipases than TYI-S-33-cultured trophozoites. Finally, trophozoites grown in any of the three tested media had similar potential to induce liver abscesses Hindawi Publishing Corporation 2018-07-11 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/17149/1/253.pdf http://eprints.uanl.mx/17149/1.haspreviewThumbnailVersion/253.pdf González Salazar, Francisco y Meester, Irene y Guzmán de la Garza, Francisco Javier y De la Garza Salinas, Laura Hermila y Sampayo Reyes, Adriana y Garza González, Jesús Norberto y Monsivais Diaz, O. y Barba Dávila, B. A. y Hernández García, Magda Elizabeth y Vargas Villarreal, Javier (2018) “CLUPS”: A New Culture Medium for the Axenic Growth of Entamoeba histolytica. Journal of Parasitology Research, 2018. pp. 1-6. ISSN 2090-0023 http://doi.org/10.1155/2018/2796516 doi:10.1155/2018/2796516 |
| spellingShingle | RC Medicina Interna, Psiquiatría, Neurología González Salazar, Francisco Meester, Irene Guzmán de la Garza, Francisco Javier De la Garza Salinas, Laura Hermila Sampayo Reyes, Adriana Garza González, Jesús Norberto Monsivais Diaz, O. Barba Dávila, B. A. Hernández García, Magda Elizabeth Vargas Villarreal, Javier “CLUPS”: A New Culture Medium for the Axenic Growth of Entamoeba histolytica |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | “CLUPS”: A New Culture Medium for the Axenic Growth of Entamoeba histolytica |
| title_full | “CLUPS”: A New Culture Medium for the Axenic Growth of Entamoeba histolytica |
| title_fullStr | “CLUPS”: A New Culture Medium for the Axenic Growth of Entamoeba histolytica |
| title_full_unstemmed | “CLUPS”: A New Culture Medium for the Axenic Growth of Entamoeba histolytica |
| title_short | “CLUPS”: A New Culture Medium for the Axenic Growth of Entamoeba histolytica |
| title_sort | clups a new culture medium for the axenic growth of entamoeba histolytica |
| topic | RC Medicina Interna, Psiquiatría, Neurología |
| url | http://eprints.uanl.mx/17149/1/253.pdf |
| work_keys_str_mv | AT gonzalezsalazarfrancisco clupsanewculturemediumfortheaxenicgrowthofentamoebahistolytica AT meesterirene clupsanewculturemediumfortheaxenicgrowthofentamoebahistolytica AT guzmandelagarzafranciscojavier clupsanewculturemediumfortheaxenicgrowthofentamoebahistolytica AT delagarzasalinaslaurahermila clupsanewculturemediumfortheaxenicgrowthofentamoebahistolytica AT sampayoreyesadriana clupsanewculturemediumfortheaxenicgrowthofentamoebahistolytica AT garzagonzalezjesusnorberto clupsanewculturemediumfortheaxenicgrowthofentamoebahistolytica AT monsivaisdiazo clupsanewculturemediumfortheaxenicgrowthofentamoebahistolytica AT barbadavilaba clupsanewculturemediumfortheaxenicgrowthofentamoebahistolytica AT hernandezgarciamagdaelizabeth clupsanewculturemediumfortheaxenicgrowthofentamoebahistolytica AT vargasvillarrealjavier clupsanewculturemediumfortheaxenicgrowthofentamoebahistolytica |