Antitumor activity of Chlorella sorokiniana and Scenedesmus sp. microalgae native of Nuevo León State, México
ABSTRACT Cancer cases result in 13% of all deaths worldwide. Unwanted side effects in patients underconventionaltreatmentshaveledtothesearchforbeneficialalternativetherapies. Microalgae synthesize compounds with known invitro and invivo biological activity againstdifferenttumorcelllines.Therefore,na...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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2018
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| Online Access: | http://eprints.uanl.mx/16340/1/188.pdf |
| _version_ | 1824414582567862272 |
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| author | Reyna Martinez, Raul Gómez Flores, Ricardo Alberto Lopez Chuken, Ulrico Javier Quintanilla Licea, Ramiro Caballero Hernández, Diana Elia Rodríguez Padilla, Cristina Beltrán Rocha, Julio César Tamez Guerra, Patricia |
| author_facet | Reyna Martinez, Raul Gómez Flores, Ricardo Alberto Lopez Chuken, Ulrico Javier Quintanilla Licea, Ramiro Caballero Hernández, Diana Elia Rodríguez Padilla, Cristina Beltrán Rocha, Julio César Tamez Guerra, Patricia |
| author_sort | Reyna Martinez, Raul |
| collection | Repositorio Institucional |
| description | ABSTRACT Cancer cases result in 13% of all deaths worldwide. Unwanted side effects in patients underconventionaltreatmentshaveledtothesearchforbeneficialalternativetherapies. Microalgae synthesize compounds with known invitro and invivo biological activity againstdifferenttumorcelllines.Therefore,nativemicroalgaefromtheStateofNuevo Leon, Mexico may become a potential source of antitumor agents. The aim of the present study was to evaluate the invitro cytotoxic effect of Nuevo Leon regional Chlorella sorokiniana (Chlorellales: Chlorellaceae) and Scenedesmus sp. (Chlorococcales: Scenedesmaceae). Native microalgae crude organic extracts cytotoxicity against murine L5178Y-R lymphoma cell line and normal lymphocyte proliferation were evaluatedusingtheMTTreductioncolorimetricassay.Celldeathpathwaywasanalyzed byacridineorangeandethidiumbromidestaining,DNAdegradationin2%agarosegel electrophoresis and caspases activity. Results indicated significant (p<0.05) 61.89% ±3.26% and 74.77%±1.84% tumor cytotoxicity by C.sorokiniana and Scenedesmus sp.methanolextracts,respectively,at500 µg/mL,bythemechanismofapoptosis.This studycontributestoMexicanmicroalgaebiodiversityknowledgeandtheirpotentialas antitumor agent sources. |
| format | Article |
| id | eprints-16340 |
| institution | UANL |
| language | English |
| publishDate | 2018 |
| record_format | eprints |
| spelling | eprints-163402024-03-05T16:05:49Z http://eprints.uanl.mx/16340/ Antitumor activity of Chlorella sorokiniana and Scenedesmus sp. microalgae native of Nuevo León State, México Reyna Martinez, Raul Gómez Flores, Ricardo Alberto Lopez Chuken, Ulrico Javier Quintanilla Licea, Ramiro Caballero Hernández, Diana Elia Rodríguez Padilla, Cristina Beltrán Rocha, Julio César Tamez Guerra, Patricia ABSTRACT Cancer cases result in 13% of all deaths worldwide. Unwanted side effects in patients underconventionaltreatmentshaveledtothesearchforbeneficialalternativetherapies. Microalgae synthesize compounds with known invitro and invivo biological activity againstdifferenttumorcelllines.Therefore,nativemicroalgaefromtheStateofNuevo Leon, Mexico may become a potential source of antitumor agents. The aim of the present study was to evaluate the invitro cytotoxic effect of Nuevo Leon regional Chlorella sorokiniana (Chlorellales: Chlorellaceae) and Scenedesmus sp. (Chlorococcales: Scenedesmaceae). Native microalgae crude organic extracts cytotoxicity against murine L5178Y-R lymphoma cell line and normal lymphocyte proliferation were evaluatedusingtheMTTreductioncolorimetricassay.Celldeathpathwaywasanalyzed byacridineorangeandethidiumbromidestaining,DNAdegradationin2%agarosegel electrophoresis and caspases activity. Results indicated significant (p<0.05) 61.89% ±3.26% and 74.77%±1.84% tumor cytotoxicity by C.sorokiniana and Scenedesmus sp.methanolextracts,respectively,at500 µg/mL,bythemechanismofapoptosis.This studycontributestoMexicanmicroalgaebiodiversityknowledgeandtheirpotentialas antitumor agent sources. 2018-02-09 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/16340/1/188.pdf http://eprints.uanl.mx/16340/1.haspreviewThumbnailVersion/188.pdf Reyna Martinez, Raul y Gómez Flores, Ricardo Alberto y Lopez Chuken, Ulrico Javier y Quintanilla Licea, Ramiro y Caballero Hernández, Diana Elia y Rodríguez Padilla, Cristina y Beltrán Rocha, Julio César y Tamez Guerra, Patricia (2018) Antitumor activity of Chlorella sorokiniana and Scenedesmus sp. microalgae native of Nuevo León State, México. PeerJ, 6. pp. 1-15. ISSN 2167-8359 http://doi.org/10.7717/peerj.4358 doi:10.7717/peerj.4358 |
| spellingShingle | Reyna Martinez, Raul Gómez Flores, Ricardo Alberto Lopez Chuken, Ulrico Javier Quintanilla Licea, Ramiro Caballero Hernández, Diana Elia Rodríguez Padilla, Cristina Beltrán Rocha, Julio César Tamez Guerra, Patricia Antitumor activity of Chlorella sorokiniana and Scenedesmus sp. microalgae native of Nuevo León State, México |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Antitumor activity of Chlorella sorokiniana and Scenedesmus sp. microalgae native of Nuevo León State, México |
| title_full | Antitumor activity of Chlorella sorokiniana and Scenedesmus sp. microalgae native of Nuevo León State, México |
| title_fullStr | Antitumor activity of Chlorella sorokiniana and Scenedesmus sp. microalgae native of Nuevo León State, México |
| title_full_unstemmed | Antitumor activity of Chlorella sorokiniana and Scenedesmus sp. microalgae native of Nuevo León State, México |
| title_short | Antitumor activity of Chlorella sorokiniana and Scenedesmus sp. microalgae native of Nuevo León State, México |
| title_sort | antitumor activity of chlorella sorokiniana and scenedesmus sp microalgae native of nuevo leon state mexico |
| url | http://eprints.uanl.mx/16340/1/188.pdf |
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