| Sumario: | ABSTRACT Cancer cases result in 13% of all deaths worldwide. Unwanted side effects in patients underconventionaltreatmentshaveledtothesearchforbeneficialalternativetherapies. Microalgae synthesize compounds with known invitro and invivo biological activity againstdifferenttumorcelllines.Therefore,nativemicroalgaefromtheStateofNuevo Leon, Mexico may become a potential source of antitumor agents. The aim of the present study was to evaluate the invitro cytotoxic effect of Nuevo Leon regional Chlorella sorokiniana (Chlorellales: Chlorellaceae) and Scenedesmus sp. (Chlorococcales: Scenedesmaceae). Native microalgae crude organic extracts cytotoxicity against murine L5178Y-R lymphoma cell line and normal lymphocyte proliferation were evaluatedusingtheMTTreductioncolorimetricassay.Celldeathpathwaywasanalyzed byacridineorangeandethidiumbromidestaining,DNAdegradationin2%agarosegel electrophoresis and caspases activity. Results indicated significant (p<0.05) 61.89% ±3.26% and 74.77%±1.84% tumor cytotoxicity by C.sorokiniana and Scenedesmus sp.methanolextracts,respectively,at500 µg/mL,bythemechanismofapoptosis.This studycontributestoMexicanmicroalgaebiodiversityknowledgeandtheirpotentialas antitumor agent sources.
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