Virucidal and Synergistic Activity of Polyphenol-Rich Extracts of Seaweeds against Measles Virus
Abstract: Although preventable by vaccination, Measles still causes thousands of deaths among young children worldwide. The discovery of new antivirals is a good approach to control new outbreaks that cause such death. In this study, we tested the antiviral activity against Measles virus (MeV)ofPoly...
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| Format: | Article |
| Language: | English |
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2018
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| Online Access: | http://eprints.uanl.mx/16108/1/81.pdf |
| _version_ | 1824414501386059776 |
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| author | Morán Santibáñez, Karla Selene Peña Hernández, Mario A. Cruz Suárez, Lucía Elizabeth Ricque Marie, Denis Skouta, Rachid Vasquez, Abimael H. Rodríguez Padilla, Cristina Trejo Ávila, Laura M. |
| author_facet | Morán Santibáñez, Karla Selene Peña Hernández, Mario A. Cruz Suárez, Lucía Elizabeth Ricque Marie, Denis Skouta, Rachid Vasquez, Abimael H. Rodríguez Padilla, Cristina Trejo Ávila, Laura M. |
| author_sort | Morán Santibáñez, Karla Selene |
| collection | Repositorio Institucional |
| description | Abstract: Although preventable by vaccination, Measles still causes thousands of deaths among young children worldwide. The discovery of new antivirals is a good approach to control new outbreaks that cause such death. In this study, we tested the antiviral activity against Measles virus (MeV)ofPolyphenol-richextracts(PPs)comingfromfiveseaweedscollectedandcultivatedinMexico. AnMTTassaywasperformedtodeterminecytotoxicityeffect,andantiviralactivitywasmeasuredby syncytia reduction assay and confirmed by qPCR. PPs from Ecklonia arborea (formerly Eisenia arborea, Phaeophyceae) and Solieriafiliformis (Rhodophyta) showed the highest Selectivity Index (SI), >3750 and >576.9 respectively. Both PPs extracts were selected to the subsequent experiments owing to their high efficacy and low cytotoxicity compared with ribavirin (SI of 11.57). The combinational effect of PPs with sulphated polysaccharides (SPs) and ribavirin were calculated by using Compusyn software. SynergisticactivitywasobservedbycombiningbothPPswithlowconcentrationsofSolieria filiformis SPs (0.01 µg/mL). The antiviral activity of the best combinations was confirmed by qPCR. Virucidal assay, time of addition, and viral penetration evaluations suggested that PPs act mainly by inactivating the viral particle. To our knowledge, this is the first report of the virucidal effect of Polyphenol-rich extracts of seaweeds. |
| format | Article |
| id | eprints-16108 |
| institution | UANL |
| language | English |
| publishDate | 2018 |
| record_format | eprints |
| spelling | eprints-161082019-06-27T20:03:47Z http://eprints.uanl.mx/16108/ Virucidal and Synergistic Activity of Polyphenol-Rich Extracts of Seaweeds against Measles Virus Morán Santibáñez, Karla Selene Peña Hernández, Mario A. Cruz Suárez, Lucía Elizabeth Ricque Marie, Denis Skouta, Rachid Vasquez, Abimael H. Rodríguez Padilla, Cristina Trejo Ávila, Laura M. QH Historia Natural, Biología QR Microbiología Abstract: Although preventable by vaccination, Measles still causes thousands of deaths among young children worldwide. The discovery of new antivirals is a good approach to control new outbreaks that cause such death. In this study, we tested the antiviral activity against Measles virus (MeV)ofPolyphenol-richextracts(PPs)comingfromfiveseaweedscollectedandcultivatedinMexico. AnMTTassaywasperformedtodeterminecytotoxicityeffect,andantiviralactivitywasmeasuredby syncytia reduction assay and confirmed by qPCR. PPs from Ecklonia arborea (formerly Eisenia arborea, Phaeophyceae) and Solieriafiliformis (Rhodophyta) showed the highest Selectivity Index (SI), >3750 and >576.9 respectively. Both PPs extracts were selected to the subsequent experiments owing to their high efficacy and low cytotoxicity compared with ribavirin (SI of 11.57). The combinational effect of PPs with sulphated polysaccharides (SPs) and ribavirin were calculated by using Compusyn software. SynergisticactivitywasobservedbycombiningbothPPswithlowconcentrationsofSolieria filiformis SPs (0.01 µg/mL). The antiviral activity of the best combinations was confirmed by qPCR. Virucidal assay, time of addition, and viral penetration evaluations suggested that PPs act mainly by inactivating the viral particle. To our knowledge, this is the first report of the virucidal effect of Polyphenol-rich extracts of seaweeds. 2018-08-30 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/16108/1/81.pdf http://eprints.uanl.mx/16108/1.haspreviewThumbnailVersion/81.pdf Morán Santibáñez, Karla Selene y Peña Hernández, Mario A. y Cruz Suárez, Lucía Elizabeth y Ricque Marie, Denis y Skouta, Rachid y Vasquez, Abimael H. y Rodríguez Padilla, Cristina y Trejo Ávila, Laura M. (2018) Virucidal and Synergistic Activity of Polyphenol-Rich Extracts of Seaweeds against Measles Virus. Viruses, 10 (9). pp. 1-14. ISSN 1999-4915 http://doi.org/10.3390/v10090465 doi:10.3390/v10090465 |
| spellingShingle | QH Historia Natural, Biología QR Microbiología Morán Santibáñez, Karla Selene Peña Hernández, Mario A. Cruz Suárez, Lucía Elizabeth Ricque Marie, Denis Skouta, Rachid Vasquez, Abimael H. Rodríguez Padilla, Cristina Trejo Ávila, Laura M. Virucidal and Synergistic Activity of Polyphenol-Rich Extracts of Seaweeds against Measles Virus |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Virucidal and Synergistic Activity of Polyphenol-Rich Extracts of Seaweeds against Measles Virus |
| title_full | Virucidal and Synergistic Activity of Polyphenol-Rich Extracts of Seaweeds against Measles Virus |
| title_fullStr | Virucidal and Synergistic Activity of Polyphenol-Rich Extracts of Seaweeds against Measles Virus |
| title_full_unstemmed | Virucidal and Synergistic Activity of Polyphenol-Rich Extracts of Seaweeds against Measles Virus |
| title_short | Virucidal and Synergistic Activity of Polyphenol-Rich Extracts of Seaweeds against Measles Virus |
| title_sort | virucidal and synergistic activity of polyphenol rich extracts of seaweeds against measles virus |
| topic | QH Historia Natural, Biología QR Microbiología |
| url | http://eprints.uanl.mx/16108/1/81.pdf |
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