Quantification of nitric oxide by high‑performance liquid chromatography‑fluorometric method in subgenomic hepatitis C virus‑replicon expressing Huh7 cells upon treatment with acetylsalicylic acid
As nitric oxide (NO) expression levels are lower in hepatocytes compared with other cell types, it is difficult to quantify this compound via Griess assay. The aim of the present study was to quantify NO concentration in the cell culture medium from a subgenomic hepatitis C virus (HCV)‑replicon expr...
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| Format: | Article |
| Language: | English |
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2018
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| Online Access: | http://eprints.uanl.mx/16105/1/79.pdf |
| _version_ | 1824414500120428544 |
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| author | Ríos Ibarra, Clara Patricia Torres de la Cruz, Víctor Manuel Ochoa Ruiz, Andrea Gabriela Rivas Estilla, Ana María |
| author_facet | Ríos Ibarra, Clara Patricia Torres de la Cruz, Víctor Manuel Ochoa Ruiz, Andrea Gabriela Rivas Estilla, Ana María |
| author_sort | Ríos Ibarra, Clara Patricia |
| collection | Repositorio Institucional |
| description | As nitric oxide (NO) expression levels are lower in hepatocytes compared with other cell types, it is difficult to quantify this compound via Griess assay. The aim of the present study was to quantify NO concentration in the cell culture medium from a subgenomic hepatitis C virus (HCV)‑replicon expressing Huh‑7 cell system using a high‑performance liquid chromatography (HPLC)‑fluorescence detector in the presence or absence of acetylsalicylic acid (ASA) treatment. HCV‑replicon cells were incubated with ASA (4 mM) for 24, 48 and 72 h. Thereafter, the medium was collected to measure nitrites (NO2-) as an indirect indicator of NO levels using diaminonaphtalene as a derivate agent. NO levels were significantly higher (1.7‑fold) in Huh‑7 replicon cells treated with ASA (72 h post‑treatment) than untreated cells (P<0.05); NO inhibitor reduced ~30% the level of NO in Huh‑7 replicon cells treated with ASA (48 h post‑treatment; P<0.05). The findings suggested that the HPLC‑fluorescence method provided an accurate and efficient measurement of NO production in Huh‑7‑HCV‑replicon cells culture medium. |
| format | Article |
| id | eprints-16105 |
| institution | UANL |
| language | English |
| publishDate | 2018 |
| record_format | eprints |
| spelling | eprints-161052019-06-28T20:09:23Z http://eprints.uanl.mx/16105/ Quantification of nitric oxide by high‑performance liquid chromatography‑fluorometric method in subgenomic hepatitis C virus‑replicon expressing Huh7 cells upon treatment with acetylsalicylic acid Ríos Ibarra, Clara Patricia Torres de la Cruz, Víctor Manuel Ochoa Ruiz, Andrea Gabriela Rivas Estilla, Ana María QR Microbiología RC Medicina Interna, Psiquiatría, Neurología As nitric oxide (NO) expression levels are lower in hepatocytes compared with other cell types, it is difficult to quantify this compound via Griess assay. The aim of the present study was to quantify NO concentration in the cell culture medium from a subgenomic hepatitis C virus (HCV)‑replicon expressing Huh‑7 cell system using a high‑performance liquid chromatography (HPLC)‑fluorescence detector in the presence or absence of acetylsalicylic acid (ASA) treatment. HCV‑replicon cells were incubated with ASA (4 mM) for 24, 48 and 72 h. Thereafter, the medium was collected to measure nitrites (NO2-) as an indirect indicator of NO levels using diaminonaphtalene as a derivate agent. NO levels were significantly higher (1.7‑fold) in Huh‑7 replicon cells treated with ASA (72 h post‑treatment) than untreated cells (P<0.05); NO inhibitor reduced ~30% the level of NO in Huh‑7 replicon cells treated with ASA (48 h post‑treatment; P<0.05). The findings suggested that the HPLC‑fluorescence method provided an accurate and efficient measurement of NO production in Huh‑7‑HCV‑replicon cells culture medium. 2018 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/16105/1/79.pdf http://eprints.uanl.mx/16105/1.haspreviewThumbnailVersion/79.pdf Ríos Ibarra, Clara Patricia y Torres de la Cruz, Víctor Manuel y Ochoa Ruiz, Andrea Gabriela y Rivas Estilla, Ana María (2018) Quantification of nitric oxide by high‑performance liquid chromatography‑fluorometric method in subgenomic hepatitis C virus‑replicon expressing Huh7 cells upon treatment with acetylsalicylic acid. Experimental and Therapeutic Medicine. pp. 2622-2626. ISSN 1792-0981 http://doi.org/10.3892/etm.2018.6515 doi:10.3892/etm.2018.6515 |
| spellingShingle | QR Microbiología RC Medicina Interna, Psiquiatría, Neurología Ríos Ibarra, Clara Patricia Torres de la Cruz, Víctor Manuel Ochoa Ruiz, Andrea Gabriela Rivas Estilla, Ana María Quantification of nitric oxide by high‑performance liquid chromatography‑fluorometric method in subgenomic hepatitis C virus‑replicon expressing Huh7 cells upon treatment with acetylsalicylic acid |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Quantification of nitric oxide by high‑performance liquid chromatography‑fluorometric method in subgenomic hepatitis C virus‑replicon expressing Huh7 cells upon treatment with acetylsalicylic acid |
| title_full | Quantification of nitric oxide by high‑performance liquid chromatography‑fluorometric method in subgenomic hepatitis C virus‑replicon expressing Huh7 cells upon treatment with acetylsalicylic acid |
| title_fullStr | Quantification of nitric oxide by high‑performance liquid chromatography‑fluorometric method in subgenomic hepatitis C virus‑replicon expressing Huh7 cells upon treatment with acetylsalicylic acid |
| title_full_unstemmed | Quantification of nitric oxide by high‑performance liquid chromatography‑fluorometric method in subgenomic hepatitis C virus‑replicon expressing Huh7 cells upon treatment with acetylsalicylic acid |
| title_short | Quantification of nitric oxide by high‑performance liquid chromatography‑fluorometric method in subgenomic hepatitis C virus‑replicon expressing Huh7 cells upon treatment with acetylsalicylic acid |
| title_sort | quantification of nitric oxide by high performance liquid chromatography fluorometric method in subgenomic hepatitis c virus replicon expressing huh7 cells upon treatment with acetylsalicylic acid |
| topic | QR Microbiología RC Medicina Interna, Psiquiatría, Neurología |
| url | http://eprints.uanl.mx/16105/1/79.pdf |
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