Nocardia brasiliensis Induces Formation of Foamy Macrophages and Dendritic Cells In Vitro and In Vivo
Foamy cells have been described in various infectious diseases, for example in actinomycetoma induced by Nocardia brasiliensis. These cells are generally considered to be macrophages, although they present dendritic cell (DC)-specific surface markers. In this study, we determined and confirmed the l...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science
2014
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| Online Access: | http://eprints.uanl.mx/15131/1/242.pdf |
| _version_ | 1824414271264522240 |
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| author | Coers, Jörn Meester, Irene Rosas Taraco, Adrián Geovanni Salinas Carmona, Mario César |
| author_facet | Coers, Jörn Meester, Irene Rosas Taraco, Adrián Geovanni Salinas Carmona, Mario César |
| author_sort | Coers, Jörn |
| collection | Repositorio Institucional |
| description | Foamy cells have been described in various infectious diseases, for example in actinomycetoma induced by Nocardia brasiliensis. These cells are generally considered to be macrophages, although they present dendritic cell (DC)-specific surface markers. In this study, we determined and confirmed the lineage of possible precursors of foamy cells in vitro and in vivo using an experimental actinomycetoma model in BALB/c mice. Bone marrow-derived macrophages (BMDM) or DC
(BMDC) were infected in vitro with N. brasiliensis or labeled with carboxyfluorescein diacetate succinimidyl ester (CFSE). Both, macrophages and DC, differentiated into foamy cells after in vitro infection. CFSE-labeled BMDM or BMDC were tested for phagocytosis and CD11c/CD11b receptors markers expression before being transferred into the actinomycetoma lesion site of infected mice. In vivo studies showed that BMDM and BMDC were traced at the site where foamy cells are present in the experimental actinomycetoma. Interestingly, many of the transferred BMDM and BMDC were stained with the lipiddroplet
fluorophore Nile Red. In conclusion, macrophages and DC cells can be differentiated into foamy cells in vitro and
in vivo during N. brasiliensis infection. |
| format | Article |
| id | eprints-15131 |
| institution | UANL |
| language | English |
| publishDate | 2014 |
| publisher | Public Library of Science |
| record_format | eprints |
| spelling | eprints-151312020-03-05T23:01:12Z http://eprints.uanl.mx/15131/ Nocardia brasiliensis Induces Formation of Foamy Macrophages and Dendritic Cells In Vitro and In Vivo Coers, Jörn Meester, Irene Rosas Taraco, Adrián Geovanni Salinas Carmona, Mario César Foamy cells have been described in various infectious diseases, for example in actinomycetoma induced by Nocardia brasiliensis. These cells are generally considered to be macrophages, although they present dendritic cell (DC)-specific surface markers. In this study, we determined and confirmed the lineage of possible precursors of foamy cells in vitro and in vivo using an experimental actinomycetoma model in BALB/c mice. Bone marrow-derived macrophages (BMDM) or DC (BMDC) were infected in vitro with N. brasiliensis or labeled with carboxyfluorescein diacetate succinimidyl ester (CFSE). Both, macrophages and DC, differentiated into foamy cells after in vitro infection. CFSE-labeled BMDM or BMDC were tested for phagocytosis and CD11c/CD11b receptors markers expression before being transferred into the actinomycetoma lesion site of infected mice. In vivo studies showed that BMDM and BMDC were traced at the site where foamy cells are present in the experimental actinomycetoma. Interestingly, many of the transferred BMDM and BMDC were stained with the lipiddroplet fluorophore Nile Red. In conclusion, macrophages and DC cells can be differentiated into foamy cells in vitro and in vivo during N. brasiliensis infection. Public Library of Science 2014 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/15131/1/242.pdf http://eprints.uanl.mx/15131/1.haspreviewThumbnailVersion/242.pdf Coers, Jörn y Meester, Irene y Rosas Taraco, Adrián Geovanni y Salinas Carmona, Mario César (2014) Nocardia brasiliensis Induces Formation of Foamy Macrophages and Dendritic Cells In Vitro and In Vivo. PloS one, 9 (6). e100064. ISSN 1932-6203 doi:10.1371/journal.pone.0100064 |
| spellingShingle | Coers, Jörn Meester, Irene Rosas Taraco, Adrián Geovanni Salinas Carmona, Mario César Nocardia brasiliensis Induces Formation of Foamy Macrophages and Dendritic Cells In Vitro and In Vivo |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Nocardia brasiliensis Induces Formation of Foamy Macrophages and Dendritic Cells In Vitro and In Vivo |
| title_full | Nocardia brasiliensis Induces Formation of Foamy Macrophages and Dendritic Cells In Vitro and In Vivo |
| title_fullStr | Nocardia brasiliensis Induces Formation of Foamy Macrophages and Dendritic Cells In Vitro and In Vivo |
| title_full_unstemmed | Nocardia brasiliensis Induces Formation of Foamy Macrophages and Dendritic Cells In Vitro and In Vivo |
| title_short | Nocardia brasiliensis Induces Formation of Foamy Macrophages and Dendritic Cells In Vitro and In Vivo |
| title_sort | nocardia brasiliensis induces formation of foamy macrophages and dendritic cells in vitro and in vivo |
| url | http://eprints.uanl.mx/15131/1/242.pdf |
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