Epidermal growth factor enhances osteogenic differentiation of dental pulp stem cells in vitro
Introduction: Epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) play an important role in extracellular matrix mineralization, a complex process required for proper bone regeneration, one of the biggest challenges in dentistry. The purpose of this study was to evaluate the os...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
2015
|
| Online Access: | http://eprints.uanl.mx/14946/1/117.pdf |
| _version_ | 1838550987096195072 |
|---|---|
| author | Del Angel Mosqueda, Casiano Gutiérrez Puente, Yolanda López Lozano, Ada Pricila Romero Zavaleta, Ricardo Emmanuel Mendiola Jiménez, Andrés Medina de la Garza, Carlos Eduardo Márquez M, Marcela De la Garza Ramos, Myriam Angélica |
| author_facet | Del Angel Mosqueda, Casiano Gutiérrez Puente, Yolanda López Lozano, Ada Pricila Romero Zavaleta, Ricardo Emmanuel Mendiola Jiménez, Andrés Medina de la Garza, Carlos Eduardo Márquez M, Marcela De la Garza Ramos, Myriam Angélica |
| author_sort | Del Angel Mosqueda, Casiano |
| collection | Repositorio Institucional |
| description | Introduction: Epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) play an important role in
extracellular matrix mineralization, a complex process required for proper bone regeneration, one of the biggest
challenges in dentistry. The purpose of this study was to evaluate the osteogenic potential of EGF and bFGF on
dental pulp stem cells (DPSCs).
Material and methods: Human DPSCs were isolated using CD105 magnetic microbeads and characterized by flow
cytometry. To induce osteoblast differentiation, the cells were cultured in osteogenic medium supplemented with EGF or bFGF at a low concentration. Cell morphology and expression of CD146 and CD10 surface markers were
analyzed using fluorescence microscopy. To measure mineralization, an alizarin red S assay was performed and
typical markers of osteoblastic phenotype were evaluated by RT-PCR.
Results: EGF treatment induced morphological changes and suppression of CD146 and CD10 markers. Additionally, the
cells were capable of producing calcium deposits and increasing the mRNA expression to alkaline phosphatase (ALP) and osteocalcin (OCN) in relation to control groups (p < 0.001). However, bFGF treatment showed an inhibitory effect.
Conclusion: These data suggests that DPSCs in combination with EGF could be an effective stem cell-based therapy for bone tissue engineering applications in periodontics and oral implantology. |
| format | Article |
| id | eprints-14946 |
| institution | UANL |
| language | English |
| publishDate | 2015 |
| record_format | eprints |
| spelling | eprints-149462025-07-23T17:00:27Z http://eprints.uanl.mx/14946/ Epidermal growth factor enhances osteogenic differentiation of dental pulp stem cells in vitro Del Angel Mosqueda, Casiano Gutiérrez Puente, Yolanda López Lozano, Ada Pricila Romero Zavaleta, Ricardo Emmanuel Mendiola Jiménez, Andrés Medina de la Garza, Carlos Eduardo Márquez M, Marcela De la Garza Ramos, Myriam Angélica Introduction: Epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) play an important role in extracellular matrix mineralization, a complex process required for proper bone regeneration, one of the biggest challenges in dentistry. The purpose of this study was to evaluate the osteogenic potential of EGF and bFGF on dental pulp stem cells (DPSCs). Material and methods: Human DPSCs were isolated using CD105 magnetic microbeads and characterized by flow cytometry. To induce osteoblast differentiation, the cells were cultured in osteogenic medium supplemented with EGF or bFGF at a low concentration. Cell morphology and expression of CD146 and CD10 surface markers were analyzed using fluorescence microscopy. To measure mineralization, an alizarin red S assay was performed and typical markers of osteoblastic phenotype were evaluated by RT-PCR. Results: EGF treatment induced morphological changes and suppression of CD146 and CD10 markers. Additionally, the cells were capable of producing calcium deposits and increasing the mRNA expression to alkaline phosphatase (ALP) and osteocalcin (OCN) in relation to control groups (p < 0.001). However, bFGF treatment showed an inhibitory effect. Conclusion: These data suggests that DPSCs in combination with EGF could be an effective stem cell-based therapy for bone tissue engineering applications in periodontics and oral implantology. 2015 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/14946/1/117.pdf http://eprints.uanl.mx/14946/1.haspreviewThumbnailVersion/117.pdf Del Angel Mosqueda, Casiano y Gutiérrez Puente, Yolanda y López Lozano, Ada Pricila y Romero Zavaleta, Ricardo Emmanuel y Mendiola Jiménez, Andrés y Medina de la Garza, Carlos Eduardo y Márquez M, Marcela y De la Garza Ramos, Myriam Angélica (2015) Epidermal growth factor enhances osteogenic differentiation of dental pulp stem cells in vitro. Head & Face Medicine, 11 (1). ISSN 1746-160X http://doi.org/10.1186/s13005-015-0086-5 doi:10.1186/s13005-015-0086-5 |
| spellingShingle | Del Angel Mosqueda, Casiano Gutiérrez Puente, Yolanda López Lozano, Ada Pricila Romero Zavaleta, Ricardo Emmanuel Mendiola Jiménez, Andrés Medina de la Garza, Carlos Eduardo Márquez M, Marcela De la Garza Ramos, Myriam Angélica Epidermal growth factor enhances osteogenic differentiation of dental pulp stem cells in vitro |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Epidermal growth factor enhances osteogenic differentiation of dental pulp stem cells in vitro |
| title_full | Epidermal growth factor enhances osteogenic differentiation of dental pulp stem cells in vitro |
| title_fullStr | Epidermal growth factor enhances osteogenic differentiation of dental pulp stem cells in vitro |
| title_full_unstemmed | Epidermal growth factor enhances osteogenic differentiation of dental pulp stem cells in vitro |
| title_short | Epidermal growth factor enhances osteogenic differentiation of dental pulp stem cells in vitro |
| title_sort | epidermal growth factor enhances osteogenic differentiation of dental pulp stem cells in vitro |
| url | http://eprints.uanl.mx/14946/1/117.pdf |
| work_keys_str_mv | AT delangelmosquedacasiano epidermalgrowthfactorenhancesosteogenicdifferentiationofdentalpulpstemcellsinvitro AT gutierrezpuenteyolanda epidermalgrowthfactorenhancesosteogenicdifferentiationofdentalpulpstemcellsinvitro AT lopezlozanoadapricila epidermalgrowthfactorenhancesosteogenicdifferentiationofdentalpulpstemcellsinvitro AT romerozavaletaricardoemmanuel epidermalgrowthfactorenhancesosteogenicdifferentiationofdentalpulpstemcellsinvitro AT mendiolajimenezandres epidermalgrowthfactorenhancesosteogenicdifferentiationofdentalpulpstemcellsinvitro AT medinadelagarzacarloseduardo epidermalgrowthfactorenhancesosteogenicdifferentiationofdentalpulpstemcellsinvitro AT marquezmmarcela epidermalgrowthfactorenhancesosteogenicdifferentiationofdentalpulpstemcellsinvitro AT delagarzaramosmyriamangelica epidermalgrowthfactorenhancesosteogenicdifferentiationofdentalpulpstemcellsinvitro |