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1061
Aislamiento y selección de cepas mutantes de Hirsutella citriformis inducidas por el agente químico: metanosulfonato de etilo (EMS).
Publicado 2017“…Techniques such as biphasic culture on different low-cost substrates (sorghum, rice and oats) were evaluated to obtain the highest conidia production that would be used in classical mutagenesis with the chemical agent ethyl methane sulfonate (EMS) which has been successfully used to introduce changes of a single base in the DNA, a mutation protocol was established varying times and concentrations for the obtaining of mutant strains, which were characterized based on radial growth, production of conidia and speed of germination in media as PDA and PDA with yeast extract. …”
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Tesis -
1062
Aislamiento y selección de cepas mutantes de Hirsutella citriformis inducidas por el agente químico: metanosulfonato de etilo (EMS).
Publicado 2017“…Techniques such as biphasic culture on different low-cost substrates (sorghum, rice and oats) were evaluated to obtain the highest conidia production that would be used in classical mutagenesis with the chemical agent ethyl methane sulfonate (EMS) which has been successfully used to introduce changes of a single base in the DNA, a mutation protocol was established varying times and concentrations for the obtaining of mutant strains, which were characterized based on radial growth, production of conidia and speed of germination in media as PDA and PDA with yeast extract. …”
Enlace del recurso
Tesis