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Análisis de expresión de genes de Mycobacterium tuberculosis durante la infección a macrófagos
Publicado 2006“…THP-1 cells monolayers differentiated to macrophages by phorbol myristate acetate (PMA) were infected with M. tuberculosis strains CDC1551, H37Rv and DR689 with a variable multiplicity of infection (MOI): 10:1, 1:1 and 0.1:1 (bacteria:macrophage) and incubated at various time points. Cytotoxicity was observed as areas of clearing and cell agglomeration and the effect was dependent on bacilli inoculum and incubation time. …”
Enlace del recurso
Tesis -
243
Análisis de expresión de genes de Mycobacterium tuberculosis durante la infección a macrófagos
Publicado 2006“…THP-1 cells monolayers differentiated to macrophages by phorbol myristate acetate (PMA) were infected with M. tuberculosis strains CDC1551, H37Rv and DR689 with a variable multiplicity of infection (MOI): 10:1, 1:1 and 0.1:1 (bacteria:macrophage) and incubated at various time points. Cytotoxicity was observed as areas of clearing and cell agglomeration and the effect was dependent on bacilli inoculum and incubation time. …”
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Selección de genes especie específicos de Mycobacterium tuberculosis expresados en condiciones de estrés térmico como candidatos para el diagnóstico de la tuberculosis.
Publicado 2018“…Then, bacteria were harvested and RNA isolation and cDNA synthesis were performed subsequently. End point PCR amplification was carried on with specific primers designed for each selected gene. …”
Enlace del recurso
Tesis -
256
Selección de genes especie específicos de Mycobacterium tuberculosis expresados en condiciones de estrés térmico como candidatos para el diagnóstico de la tuberculosis.
Publicado 2018“…Then, bacteria were harvested and RNA isolation and cDNA synthesis were performed subsequently. End point PCR amplification was carried on with specific primers designed for each selected gene. …”
Enlace del recurso
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