Resultados de búsqueda - ((((line OR like) OR (nota OR (anita OR anora))) OR aiza) OR (epiza OR elisa))

  1. 301

    Gene regulatory network underlying the immortalization of epithelial cells por Méndez López, Luis Fernando, Dávila Velderrain, José, Domínguez Hüttinger, Elisa, Enríquez Olguín, Christian, Martínez García, Juan Carlos, Alvarez Buylla Roces, María Elena

    Publicado 2017
    “…Abstract Background: Tumorigenic transformationofhumanepithelialcellsinvitrohasbeendescribedexperimentallyas thepotentialresultofspontaneousimmortalization.Thisprocessischaracterizedbyaseriesofcell–statetransitions,in whichnormalepithelialcellsacquirefirstasenescentstatewhichislatersurpassedtoattainamesenchymalstem–like phenotypewithapotentiallytumorigenicbehavior.Inthispaperweaimtoprovideasystem–levelmechanistic explanationtotheemergenceofthesecelltypes,andtothetime–orderedtransitionpatternsthatarecommonto neoplasiasofepithelialorigin.Tothisend,wefirstintegratepublishedfunctionalandwell–curatedmoleculardataof thecomponentsandinteractionsthathavebeenfoundtobeinvolvedinsuchcellstatesandtransitionsintoa networkof41molecularcomponents.Wethenreducethisinitialnetworkbyremovingsimplemediators(i.e.,linear pathways),andformalizetheresultingregulatorycoreintologicalrulesthatgovernthedynamicsofeachofthe networkcomponentsasafunctionofthestatesofitsregulators. …”
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    WT1 shRNA delivery using transferrin-conjugated PEG liposomes in an in vivo model of melanoma por Saavedra Alonso, Santiago, Zapata Benavides, Pablo, Chávez Escamilla, Ana Karina, Manilla Muñoz, Edgar, Zamora Ávila, Diana Elisa, Franco Molina, Moisés Armides, Rodríguez Padilla, Cristina

    Publicado 2016
    “…Formulations of transferrin (Tf)‑conjugated polyethylene glycol (PEG) liposomes loaded with short hairpin RNA (shRNA) against WT1 (Lip + RNAi + Tf), PEG liposomes loaded with shRNA against WT1 (Lip + RNAi), Tf‑conjugated PEG liposomes loaded with pEGFP‑N3 (Lip + GFP + Tf) and saline solution as negative control (untreated) were administered systemically to C57BL/6 mice implanted subcutaneously with a melanoma cell line. Tumor volume, body weight, tumor weight, survival and relative expression of WT1 were evaluated. …”
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    WT1 silencing by RNAi synergizes with chemotherapeutic agents and induces chemosensitization to doxorubicin and cisplatin in B16F10 murine melanoma cells por Zapata Benavides, Pablo, Manilla Muñoz, Edgar, Zamora Ávila, Diana Elisa, Saavedra Alonso, Santiago, Franco Molina, Moisés Armides, Trejo Ávila, Laura M., Dávalos Aranda, Guillermo, Rodríguez Padilla, Cristina

    Publicado 2012
    “…The objectives of this study were to investigate whether WT1 silencing is capable of synergizing with chemotherapeutic agents and whether this silencing is capable of sensitizing cancer cells to doxorubicin and cisplatin in the B16F10 murine melanoma cell line. In the present study, B16F10 cells were simultaneously treated with median lethal doses (LD50s) of WT1-1 or WT1-2 small hairpin RNAs (shRNAs) and chemotherapeutic agents. …”
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