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Análisis de expresión de genes de Mycobacterium tuberculosis durante la infección a macrófagos
Published 2006“…RNA was isolated from bacteria grown in Middlebrook 7H9 medium after 7 days or in RPMI medium at 2, 6 and 24 h, as well as RNA from bacteria infecting macrophages at the same time points. Selection of analysed genes was based on genes reported as probable virulence factors of M. tuberculosis or genes with undefined function in M. tuberculosis but having a nucleotide sequence homologous to virulence genes from others pathogens. …”
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Análisis de expresión de genes de Mycobacterium tuberculosis durante la infección a macrófagos
Published 2006“…RNA was isolated from bacteria grown in Middlebrook 7H9 medium after 7 days or in RPMI medium at 2, 6 and 24 h, as well as RNA from bacteria infecting macrophages at the same time points. Selection of analysed genes was based on genes reported as probable virulence factors of M. tuberculosis or genes with undefined function in M. tuberculosis but having a nucleotide sequence homologous to virulence genes from others pathogens. …”
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Thymidylate synthase gene variants as predictors of clinical response and toxicity to fluoropyrimidine-based chemotherapy for colorectal cancer
Published 2017“…This is the first study made on a Latin American population that points out TYMS gene variants have predictive values for response and toxicity in patients with CRC treated with fluoropyrimidine-based chemotherapy.…”
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Analyses of chondrogenic induction of adipose mesenchymal stem cells by combined co-stimulation mediated by adenoviral gene transfer
Published 2013“…These were harvested at various time points for detection of cartilage-specific genes expression by quantitative real-time PCR or after 14 and 28 days for histologic and biochemical analyses detecting proteoglycans, collagens (II, I and X), and total sulfated glycosaminoglycan and collagen content, respectively. …”
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Farmacorresistencia en aislamientos clínicos de Clostridium difficile asociados a los genes de resistencia cfr, rpoB y rpoC.
Published 2017“…The resistance profiles of 128 clinical isolates of C. difficile were analyzed, the presence of the cfr gene was analyzed by end-point PCR, and by PCR product sequencing the mutations related to resistance to vancomycin, rifampicin and reduction of susceptibility to fidaxomicin. …”
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Farmacorresistencia en aislamientos clínicos de Clostridium difficile asociados a los genes de resistencia cfr, rpoB y rpoC.
Published 2017“…The resistance profiles of 128 clinical isolates of C. difficile were analyzed, the presence of the cfr gene was analyzed by end-point PCR, and by PCR product sequencing the mutations related to resistance to vancomycin, rifampicin and reduction of susceptibility to fidaxomicin. …”
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Selección de genes especie específicos de Mycobacterium tuberculosis expresados en condiciones de estrés térmico como candidatos para el diagnóstico de la tuberculosis.
Published 2018“…Then, bacteria were harvested and RNA isolation and cDNA synthesis were performed subsequently. End point PCR amplification was carried on with specific primers designed for each selected gene. …”
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Selección de genes especie específicos de Mycobacterium tuberculosis expresados en condiciones de estrés térmico como candidatos para el diagnóstico de la tuberculosis.
Published 2018“…Then, bacteria were harvested and RNA isolation and cDNA synthesis were performed subsequently. End point PCR amplification was carried on with specific primers designed for each selected gene. …”
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Characterization of Trehalose-6-phosphate Synthase and Trehalose-6-phosphate Phosphatase Genes and Analysis of its Differential Expression in Maize (Zea mays) Seedlings under Droug...
Published 2020“…Relative expression of both genes was evaluated by RT-qPCR at different time points. …”
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Polimorfismo de los genes que codifican para las proteínas VP4, VP7 y NSP4 de rotavirus y su asociación con la severidad de la diarrea
Published 2006“…Abstract Of the viruses that cause gastroenteritis, rotavirus is the main causal agent of severe diarrhea; it causes the fence death 440,000 children per year at world-wide level. Some researchers point to proteins VP4, VP7 and NSP4 like pathogenicity factors; VP4 and VP7 are related with the penetration viral into epithelial cells and the synthesis of neutralization antibodies; NSP4 is the first described viral enterotoxin, which, by means of an increase in Ca2+ it triggers the diarrheic processes. …”
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Polimorfismo de los genes que codifican para las proteínas VP4, VP7 y NSP4 de rotavirus y su asociación con la severidad de la diarrea
Published 2006“…Abstract Of the viruses that cause gastroenteritis, rotavirus is the main causal agent of severe diarrhea; it causes the fence death 440,000 children per year at world-wide level. Some researchers point to proteins VP4, VP7 and NSP4 like pathogenicity factors; VP4 and VP7 are related with the penetration viral into epithelial cells and the synthesis of neutralization antibodies; NSP4 is the first described viral enterotoxin, which, by means of an increase in Ca2+ it triggers the diarrheic processes. …”
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Meta-analysis Reveals Genome-Wide Significance at 15q13 for Nonsyndromic Clefting of Both the Lip and the Palate, and Functional Analyses Implicate GREM1 As a Plausible Causative G...
Published 2016“…This entity specifically involves abnormalities of the lip and soft palate, which develop at different time-points and in separate anatomical regions.…”
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Puntos de vista de la responsabilidad social corporativa (Points of view on social corporate responsibility
Published 2005“…This study has the objective ofpresenting the differents points of view about Corporate Social Responsibility (CSR) in the business world. …”
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Las TIC como herramientas de apoyo en la enseñanza de inglés como lengua extranjera en el enfoque comunicativo.
Published 2017“…Por el contrario, se limitaron a hablar de herramientas de uso frecuente / o común como Power Point y en casos más peculiares, uso de canciones. …”
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