Characterization of Insulin-Like Peptides and Their Relation to Molt and Metabolism of the Shrimp Litopenaeus vannamei
Insulin-like peptides are key regulators of metabolism, reproduction, and senescence in higher eukaryotic organisms.They have been described in different invertebrates, including nematodes, molluscs, and insects. Insulin-like peptideshave also been detected in some decapod crustaceans, where they ma...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Facultad de Ciencias Biologicas
2008
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| Online Access: | https://nutricionacuicola.uanl.mx/index.php/acu/article/view/153 |
| _version_ | 1824324495466299392 |
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| author | Gutiérrez, Ana Nieto, Julie Pozo, Francisco VandenBosch, Luc Van Soest, Sofie Schoofs, Liliane |
| author_facet | Gutiérrez, Ana Nieto, Julie Pozo, Francisco VandenBosch, Luc Van Soest, Sofie Schoofs, Liliane |
| author_sort | Gutiérrez, Ana |
| collection | Artículos de Revistas UANL |
| description | Insulin-like peptides are key regulators of metabolism, reproduction, and senescence in higher eukaryotic organisms.They have been described in different invertebrates, including nematodes, molluscs, and insects. Insulin-like peptideshave also been detected in some decapod crustaceans, where they may display typical insulin effects. On the basisthat common biological function may be related to a similar structure, we searched for an insulin-related substance inthe shrimp Litopenaeus vannamei and further investigated its function. An IGF-I-like molecule was detected inneurosecretory cells of the brain and thoracic ganglion in shrimps at different molt stages. Furthermore, wedemonstrated that ILP did not differentially stimulate ecdysteroid secretion as it is observed in some insect species.An in vivo assay was performed and the results revealed that injections of heterologous insulin/IGF-I increased theglycogen contents of gills and digestive gland from intermolt animals (fasting state), suggesting that an endogenousILP may display a conserved function in decapods. Moreover, cloning and sequencing of a cDNA from hemocytesrevealed that the sequence code for a precursor similar to the Insulin-like Growth Factor binding proteins. Aquantitative real-time RT-PCR study revealed the presence of LivIGFBP-related peptide transcript in a variety oftissues, including nervous tissues, gills, and muscle, brain, thoracic ganglion and nerve cord. In addition, theexpression of this transcript differs according to the animal’s size, suggesting a possible role in growth regulation. |
| first_indexed | 2025-02-05T19:09:23Z |
| format | Article |
| id | nutrucionacuicola-article-153 |
| institution | UANL |
| language | eng |
| last_indexed | 2025-02-05T19:09:23Z |
| physical | Avances en Nutrición Acuicola; 2008: Memorías del Noveno Simposium Internacional de Nutrición Acuícola Noviembre 24-27, 2008 |
| publishDate | 2008 |
| publisher | Facultad de Ciencias Biologicas |
| record_format | ojs |
| spelling | nutrucionacuicola-article-1532019-10-25T23:06:00Z Characterization of Insulin-Like Peptides and Their Relation to Molt and Metabolism of the Shrimp Litopenaeus vannamei Characterization of Insulin-Like Peptides and Their Relation to Molt and Metabolism of the Shrimp Litopenaeus vannamei Gutiérrez, Ana Nieto, Julie Pozo, Francisco VandenBosch, Luc Van Soest, Sofie Schoofs, Liliane Insulin-like peptides are key regulators of metabolism, reproduction, and senescence in higher eukaryotic organisms.They have been described in different invertebrates, including nematodes, molluscs, and insects. Insulin-like peptideshave also been detected in some decapod crustaceans, where they may display typical insulin effects. On the basisthat common biological function may be related to a similar structure, we searched for an insulin-related substance inthe shrimp Litopenaeus vannamei and further investigated its function. An IGF-I-like molecule was detected inneurosecretory cells of the brain and thoracic ganglion in shrimps at different molt stages. Furthermore, wedemonstrated that ILP did not differentially stimulate ecdysteroid secretion as it is observed in some insect species.An in vivo assay was performed and the results revealed that injections of heterologous insulin/IGF-I increased theglycogen contents of gills and digestive gland from intermolt animals (fasting state), suggesting that an endogenousILP may display a conserved function in decapods. Moreover, cloning and sequencing of a cDNA from hemocytesrevealed that the sequence code for a precursor similar to the Insulin-like Growth Factor binding proteins. Aquantitative real-time RT-PCR study revealed the presence of LivIGFBP-related peptide transcript in a variety oftissues, including nervous tissues, gills, and muscle, brain, thoracic ganglion and nerve cord. In addition, theexpression of this transcript differs according to the animal’s size, suggesting a possible role in growth regulation. Insulin-like peptides are key regulators of metabolism, reproduction, and senescence in higher eukaryotic organisms.They have been described in different invertebrates, including nematodes, molluscs, and insects. Insulin-like peptideshave also been detected in some decapod crustaceans, where they may display typical insulin effects. On the basisthat common biological function may be related to a similar structure, we searched for an insulin-related substance inthe shrimp Litopenaeus vannamei and further investigated its function. An IGF-I-like molecule was detected inneurosecretory cells of the brain and thoracic ganglion in shrimps at different molt stages. Furthermore, wedemonstrated that ILP did not differentially stimulate ecdysteroid secretion as it is observed in some insect species.An in vivo assay was performed and the results revealed that injections of heterologous insulin/IGF-I increased theglycogen contents of gills and digestive gland from intermolt animals (fasting state), suggesting that an endogenousILP may display a conserved function in decapods. Moreover, cloning and sequencing of a cDNA from hemocytesrevealed that the sequence code for a precursor similar to the Insulin-like Growth Factor binding proteins. Aquantitative real-time RT-PCR study revealed the presence of LivIGFBP-related peptide transcript in a variety oftissues, including nervous tissues, gills, and muscle, brain, thoracic ganglion and nerve cord. In addition, theexpression of this transcript differs according to the animal’s size, suggesting a possible role in growth regulation. Facultad de Ciencias Biologicas 2008-11-24 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artículo revisado por pares application/pdf https://nutricionacuicola.uanl.mx/index.php/acu/article/view/153 Avances en Nutrición Acuicola; 2008: Memorías del Noveno Simposium Internacional de Nutrición Acuícola Noviembre 24-27, 2008 eng https://nutricionacuicola.uanl.mx/index.php/acu/article/view/153/151 Derechos de autor 2019 Ana Gutiérrez, Julie Nieto, Francisco Pozo, Luc VandenBosch, Sofie Van Soest, Liliane Schoofs |
| spellingShingle | Gutiérrez, Ana Nieto, Julie Pozo, Francisco VandenBosch, Luc Van Soest, Sofie Schoofs, Liliane Characterization of Insulin-Like Peptides and Their Relation to Molt and Metabolism of the Shrimp Litopenaeus vannamei |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/article.gif |
| title | Characterization of Insulin-Like Peptides and Their Relation to Molt and Metabolism of the Shrimp Litopenaeus vannamei |
| title_alt | Characterization of Insulin-Like Peptides and Their Relation to Molt and Metabolism of the Shrimp Litopenaeus vannamei |
| title_full | Characterization of Insulin-Like Peptides and Their Relation to Molt and Metabolism of the Shrimp Litopenaeus vannamei |
| title_fullStr | Characterization of Insulin-Like Peptides and Their Relation to Molt and Metabolism of the Shrimp Litopenaeus vannamei |
| title_full_unstemmed | Characterization of Insulin-Like Peptides and Their Relation to Molt and Metabolism of the Shrimp Litopenaeus vannamei |
| title_short | Characterization of Insulin-Like Peptides and Their Relation to Molt and Metabolism of the Shrimp Litopenaeus vannamei |
| title_sort | characterization of insulin like peptides and their relation to molt and metabolism of the shrimp litopenaeus vannamei |
| url | https://nutricionacuicola.uanl.mx/index.php/acu/article/view/153 |
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