An easy way to distinguish DNA from protein: an experiment for general chemistry.
We propose a classroom experiment to complement a classroom activity described by Ellis et al. in this Journal. In the Ellis activity students extracted long polymer fibers from the cells of common foods. In this experiment, the identity of the long polymer fibers as either DNA or protein can be det...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
2012
|
| Online Access: | http://eprints.uanl.mx/8139/1/l7_1.pdf |
| _version_ | 1824370643700809728 |
|---|---|
| author | López Valentín, Dulce María Pulido Cordoba, Liliana Chávez Reyes, Arturo |
| author_facet | López Valentín, Dulce María Pulido Cordoba, Liliana Chávez Reyes, Arturo |
| author_sort | López Valentín, Dulce María |
| collection | Repositorio Institucional |
| description | We propose a classroom experiment to complement a classroom activity described by Ellis et al. in this Journal. In the Ellis activity students extracted long polymer fibers from the cells of common foods. In this experiment, the identity of the long polymer fibers as either DNA or protein can be determined by doing three accessible, inexpensive, and easy tests. Two of the tests, temperature and the acid-medium effect, are based on the physicochemical properties of DNA (reversible denaturalization), and the third test is a qualitative determination of proteins (absent in DNA). The results of these three tests provide evidence to distinguish the isolated molecule of DNA from proteins that might
look like DNA. |
| format | Article |
| id | eprints-8139 |
| institution | UANL |
| language | English |
| publishDate | 2012 |
| record_format | eprints |
| spelling | eprints-81392016-12-05T15:32:58Z http://eprints.uanl.mx/8139/ An easy way to distinguish DNA from protein: an experiment for general chemistry. López Valentín, Dulce María Pulido Cordoba, Liliana Chávez Reyes, Arturo We propose a classroom experiment to complement a classroom activity described by Ellis et al. in this Journal. In the Ellis activity students extracted long polymer fibers from the cells of common foods. In this experiment, the identity of the long polymer fibers as either DNA or protein can be determined by doing three accessible, inexpensive, and easy tests. Two of the tests, temperature and the acid-medium effect, are based on the physicochemical properties of DNA (reversible denaturalization), and the third test is a qualitative determination of proteins (absent in DNA). The results of these three tests provide evidence to distinguish the isolated molecule of DNA from proteins that might look like DNA. 2012 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/8139/1/l7_1.pdf http://eprints.uanl.mx/8139/1.haspreviewThumbnailVersion/l7_1.pdf López Valentín, Dulce María y Pulido Cordoba, Liliana y Chávez Reyes, Arturo (2012) An easy way to distinguish DNA from protein: an experiment for general chemistry. Journal of Chemical Education, 89 (10). pp. 1333-1335. ISSN 0021-9584 (Entregado) |
| spellingShingle | López Valentín, Dulce María Pulido Cordoba, Liliana Chávez Reyes, Arturo An easy way to distinguish DNA from protein: an experiment for general chemistry. |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | An easy way to distinguish DNA from protein: an experiment for general chemistry. |
| title_full | An easy way to distinguish DNA from protein: an experiment for general chemistry. |
| title_fullStr | An easy way to distinguish DNA from protein: an experiment for general chemistry. |
| title_full_unstemmed | An easy way to distinguish DNA from protein: an experiment for general chemistry. |
| title_short | An easy way to distinguish DNA from protein: an experiment for general chemistry. |
| title_sort | easy way to distinguish dna from protein an experiment for general chemistry |
| url | http://eprints.uanl.mx/8139/1/l7_1.pdf |
| work_keys_str_mv | AT lopezvalentindulcemaria aneasywaytodistinguishdnafromproteinanexperimentforgeneralchemistry AT pulidocordobaliliana aneasywaytodistinguishdnafromproteinanexperimentforgeneralchemistry AT chavezreyesarturo aneasywaytodistinguishdnafromproteinanexperimentforgeneralchemistry AT lopezvalentindulcemaria easywaytodistinguishdnafromproteinanexperimentforgeneralchemistry AT pulidocordobaliliana easywaytodistinguishdnafromproteinanexperimentforgeneralchemistry AT chavezreyesarturo easywaytodistinguishdnafromproteinanexperimentforgeneralchemistry |