Detection of West Nile virus-specific antibodies and nucleic acid in horses and mosquitoes, respectively, in Nuevo Leon State, northern Mexico, 2006–2007
Abstract. In the last 5 years, there has been only one reported human case of West Nile virus (WNV) disease in northern Mexico. To determine if the virus was still circulating in this region, equine and entomological surveillance for WNV was conducted in the state of Nuevo Leon in northern Mexico in...
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| Format: | Article |
| Language: | English |
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Royal Entomological Society of London
2012
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| Online Access: | http://eprints.uanl.mx/6439/1/Detection%20of%20West%20Nile%20virus-specific%20antibodies%20and%20nucleic%20acid%20in%20horses%20and%20mosquitoes%2C%20respectively%2C%20in%20Nuevo%20Leon%20State%2C%20northern%20Mexico%2C%202006%E2%80%932007.pdf |
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| author | Ibarra Juárez, Luis Arturo Eisen, Lars Bolling, B. G. Beaty, Barry J. Blitvich, Bradley J. Sánchez Casas, Rosa María Ayala Sulca, Yuri Olivier Fernández Salas, Ildefonso |
| author_facet | Ibarra Juárez, Luis Arturo Eisen, Lars Bolling, B. G. Beaty, Barry J. Blitvich, Bradley J. Sánchez Casas, Rosa María Ayala Sulca, Yuri Olivier Fernández Salas, Ildefonso |
| author_sort | Ibarra Juárez, Luis Arturo |
| collection | Repositorio Institucional |
| description | Abstract. In the last 5 years, there has been only one reported human case of West Nile virus (WNV) disease in northern Mexico. To determine if the virus was still circulating in this region, equine and entomological surveillance for WNV was conducted in the state of Nuevo Leon in northern Mexico in 2006 and 2007. A total of 203 horses were serologically assayed for antibodies to WNV using an epitope-blocking enzyme-linked immunosorbent assay (bELISA). Seroprevalences for WNV in horses sampled in 2006 and 2007 were 26% and 45%, respectively.
Mosquito collections in 2007 produced 7365 specimens representing 15 species. Culex mosquitoes were screened for WNV RNA and other genera (Mansonia, Anopheles,
Aedes, Psorophora and Uranotaenia) were screened for flaviviruses using reversetranscription (RT)-PCR. Two pools consisting of Culex spp. mosquitoes contained
WNV RNA. Molecular species identification revealed that neither pool included Culex quinquefasciatus (Say) (Diptera:Culicidae) complex mosquitoes. No evidence
of flaviviruses was found in the other mosquito genera examined. These data provide evidence that WNV is currently circulating in northern Mexico and that non-Cx.
quinquefasciatus spp. mosquitoes may be participating in the WNV transmission cycle in this region. |
| format | Article |
| id | eprints-6439 |
| institution | UANL |
| language | English |
| publishDate | 2012 |
| publisher | Royal Entomological Society of London |
| record_format | eprints |
| spelling | eprints-64392021-05-27T15:23:52Z http://eprints.uanl.mx/6439/ Detection of West Nile virus-specific antibodies and nucleic acid in horses and mosquitoes, respectively, in Nuevo Leon State, northern Mexico, 2006–2007 Ibarra Juárez, Luis Arturo Eisen, Lars Bolling, B. G. Beaty, Barry J. Blitvich, Bradley J. Sánchez Casas, Rosa María Ayala Sulca, Yuri Olivier Fernández Salas, Ildefonso Q Ciencias en General QH Historia Natural, Biología Abstract. In the last 5 years, there has been only one reported human case of West Nile virus (WNV) disease in northern Mexico. To determine if the virus was still circulating in this region, equine and entomological surveillance for WNV was conducted in the state of Nuevo Leon in northern Mexico in 2006 and 2007. A total of 203 horses were serologically assayed for antibodies to WNV using an epitope-blocking enzyme-linked immunosorbent assay (bELISA). Seroprevalences for WNV in horses sampled in 2006 and 2007 were 26% and 45%, respectively. Mosquito collections in 2007 produced 7365 specimens representing 15 species. Culex mosquitoes were screened for WNV RNA and other genera (Mansonia, Anopheles, Aedes, Psorophora and Uranotaenia) were screened for flaviviruses using reversetranscription (RT)-PCR. Two pools consisting of Culex spp. mosquitoes contained WNV RNA. Molecular species identification revealed that neither pool included Culex quinquefasciatus (Say) (Diptera:Culicidae) complex mosquitoes. No evidence of flaviviruses was found in the other mosquito genera examined. These data provide evidence that WNV is currently circulating in northern Mexico and that non-Cx. quinquefasciatus spp. mosquitoes may be participating in the WNV transmission cycle in this region. Royal Entomological Society of London 2012 Article PeerReviewed text en http://eprints.uanl.mx/6439/1/Detection%20of%20West%20Nile%20virus-specific%20antibodies%20and%20nucleic%20acid%20in%20horses%20and%20mosquitoes%2C%20respectively%2C%20in%20Nuevo%20Leon%20State%2C%20northern%20Mexico%2C%202006%E2%80%932007.pdf http://eprints.uanl.mx/6439/1.haspreviewThumbnailVersion/Detection%20of%20West%20Nile%20virus-specific%20antibodies%20and%20nucleic%20acid%20in%20horses%20and%20mosquitoes%2C%20respectively%2C%20in%20Nuevo%20Leon%20State%2C%20northern%20Mexico%2C%202006%E2%80%932007.pdf Ibarra Juárez, Luis Arturo y Eisen, Lars y Bolling, B. G. y Beaty, Barry J. y Blitvich, Bradley J. y Sánchez Casas, Rosa María y Ayala Sulca, Yuri Olivier y Fernández Salas, Ildefonso (2012) Detection of West Nile virus-specific antibodies and nucleic acid in horses and mosquitoes, respectively, in Nuevo Leon State, northern Mexico, 2006–2007. Medical and veterinary entomology, 26 (1). pp. 351-354. ISSN 0269-283X http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2915.2012.01014.x/full |
| spellingShingle | Q Ciencias en General QH Historia Natural, Biología Ibarra Juárez, Luis Arturo Eisen, Lars Bolling, B. G. Beaty, Barry J. Blitvich, Bradley J. Sánchez Casas, Rosa María Ayala Sulca, Yuri Olivier Fernández Salas, Ildefonso Detection of West Nile virus-specific antibodies and nucleic acid in horses and mosquitoes, respectively, in Nuevo Leon State, northern Mexico, 2006–2007 |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Detection of West Nile virus-specific antibodies and
nucleic acid in horses and mosquitoes, respectively,
in Nuevo Leon State, northern Mexico, 2006–2007 |
| title_full | Detection of West Nile virus-specific antibodies and
nucleic acid in horses and mosquitoes, respectively,
in Nuevo Leon State, northern Mexico, 2006–2007 |
| title_fullStr | Detection of West Nile virus-specific antibodies and
nucleic acid in horses and mosquitoes, respectively,
in Nuevo Leon State, northern Mexico, 2006–2007 |
| title_full_unstemmed | Detection of West Nile virus-specific antibodies and
nucleic acid in horses and mosquitoes, respectively,
in Nuevo Leon State, northern Mexico, 2006–2007 |
| title_short | Detection of West Nile virus-specific antibodies and
nucleic acid in horses and mosquitoes, respectively,
in Nuevo Leon State, northern Mexico, 2006–2007 |
| title_sort | detection of west nile virus specific antibodies and nucleic acid in horses and mosquitoes respectively in nuevo leon state northern mexico 2006 2007 |
| topic | Q Ciencias en General QH Historia Natural, Biología |
| url | http://eprints.uanl.mx/6439/1/Detection%20of%20West%20Nile%20virus-specific%20antibodies%20and%20nucleic%20acid%20in%20horses%20and%20mosquitoes%2C%20respectively%2C%20in%20Nuevo%20Leon%20State%2C%20northern%20Mexico%2C%202006%E2%80%932007.pdf |
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