Detection of aedes aegypti mosquitoes infected with dengue virus as a complementary method for increasing the sensitivity of surveillance: identification of serotypes 1, 2, and 4 by rt-pcr in Quintana Roo, Mexico
Abstract. Sensitivity of monitoring Aedes aegypti (L.) populations was determined to identify the distribution of dengue virus (DENV) during epidemics in Quintana Roo. From September to November 2012, we used a motorized aspirator to collect 2,144 female Ae. aegypti from 569 homes. These were group...
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| Formato: | Artículo |
| Lenguaje: | inglés |
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Society of Southwestern Entomologists
2014
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| Acceso en línea: | http://eprints.uanl.mx/6437/1/Detection%20of%20Aedes%20aegypti%20Mosquitoes%20Infected%20with%20Dengue%20Virus%20as%20a%20Complementary%20Method%20for%20Increasing%20the%20Sensitivity%20of%20Surveillance%20Identification%20o.pdf |
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| author | Méndez Galván, Jorge Fernando Sánchez Casas, Rosa María Gaitan Burns, Alejandro Díaz González, Esteban Eduardo Ibarra Juárez, Luis Arturo Medina de la Garza, Carlos Eduardo Domínguez Galera, Marco Antonio Mis Ávila, Pedro Fernández Salas, Ildefonso |
| author_facet | Méndez Galván, Jorge Fernando Sánchez Casas, Rosa María Gaitan Burns, Alejandro Díaz González, Esteban Eduardo Ibarra Juárez, Luis Arturo Medina de la Garza, Carlos Eduardo Domínguez Galera, Marco Antonio Mis Ávila, Pedro Fernández Salas, Ildefonso |
| author_sort | Méndez Galván, Jorge Fernando |
| collection | Repositorio Institucional |
| description | Abstract. Sensitivity of monitoring Aedes aegypti (L.) populations was determined to identify the distribution of dengue virus (DENV) during epidemics in Quintana
Roo. From September to November 2012, we used a motorized aspirator to collect 2,144 female Ae. aegypti from 569 homes. These were grouped into 220 to use
semi-nested RT-PCR for DENV, and positive groups were analyzed individually. Five groups (2.27%) were positive for DENV. Individual analysis yielded eight
groups that tested positive, six with DENV-2, one DENV-1, and one DENV-4. The latter was not reported by the surveillance system that year. The mean number of female mosquitoes per household was 3.77 ± 5.71, and the rate of viral infection of Ae. aegypti was 0.4%. Most infected mosquitoes (49%) were concentrated in 10% of the houses. Monitoring Ae. aegypti infected with DENV has the potential to complement the current system of clinical and entomological surveillance. |
| format | Article |
| id | eprints-6437 |
| institution | UANL |
| language | English |
| publishDate | 2014 |
| publisher | Society of Southwestern Entomologists |
| record_format | eprints |
| spelling | eprints-64372025-07-23T17:01:09Z http://eprints.uanl.mx/6437/ Detection of aedes aegypti mosquitoes infected with dengue virus as a complementary method for increasing the sensitivity of surveillance: identification of serotypes 1, 2, and 4 by rt-pcr in Quintana Roo, Mexico Méndez Galván, Jorge Fernando Sánchez Casas, Rosa María Gaitan Burns, Alejandro Díaz González, Esteban Eduardo Ibarra Juárez, Luis Arturo Medina de la Garza, Carlos Eduardo Domínguez Galera, Marco Antonio Mis Ávila, Pedro Fernández Salas, Ildefonso Q Ciencias en General QH Historia Natural, Biología Abstract. Sensitivity of monitoring Aedes aegypti (L.) populations was determined to identify the distribution of dengue virus (DENV) during epidemics in Quintana Roo. From September to November 2012, we used a motorized aspirator to collect 2,144 female Ae. aegypti from 569 homes. These were grouped into 220 to use semi-nested RT-PCR for DENV, and positive groups were analyzed individually. Five groups (2.27%) were positive for DENV. Individual analysis yielded eight groups that tested positive, six with DENV-2, one DENV-1, and one DENV-4. The latter was not reported by the surveillance system that year. The mean number of female mosquitoes per household was 3.77 ± 5.71, and the rate of viral infection of Ae. aegypti was 0.4%. Most infected mosquitoes (49%) were concentrated in 10% of the houses. Monitoring Ae. aegypti infected with DENV has the potential to complement the current system of clinical and entomological surveillance. Society of Southwestern Entomologists 2014 Article PeerReviewed text en http://eprints.uanl.mx/6437/1/Detection%20of%20Aedes%20aegypti%20Mosquitoes%20Infected%20with%20Dengue%20Virus%20as%20a%20Complementary%20Method%20for%20Increasing%20the%20Sensitivity%20of%20Surveillance%20Identification%20o.pdf http://eprints.uanl.mx/6437/1.haspreviewThumbnailVersion/Detection%20of%20Aedes%20aegypti%20Mosquitoes%20Infected%20with%20Dengue%20Virus%20as%20a%20Complementary%20Method%20for%20Increasing%20the%20Sensitivity%20of%20Surveillance%20Identification%20o.pdf Méndez Galván, Jorge Fernando y Sánchez Casas, Rosa María y Gaitan Burns, Alejandro y Díaz González, Esteban Eduardo y Ibarra Juárez, Luis Arturo y Medina de la Garza, Carlos Eduardo y Domínguez Galera, Marco Antonio y Mis Ávila, Pedro y Fernández Salas, Ildefonso (2014) Detection of aedes aegypti mosquitoes infected with dengue virus as a complementary method for increasing the sensitivity of surveillance: identification of serotypes 1, 2, and 4 by rt-pcr in Quintana Roo, Mexico. The Southwestern entomologist, 39 (2). pp. 307-316. ISSN 0147-1724 http://www.bioone.org/doi/full/10.3958/059.039.0208 |
| spellingShingle | Q Ciencias en General QH Historia Natural, Biología Méndez Galván, Jorge Fernando Sánchez Casas, Rosa María Gaitan Burns, Alejandro Díaz González, Esteban Eduardo Ibarra Juárez, Luis Arturo Medina de la Garza, Carlos Eduardo Domínguez Galera, Marco Antonio Mis Ávila, Pedro Fernández Salas, Ildefonso Detection of aedes aegypti mosquitoes infected with dengue virus as a complementary method for increasing the sensitivity of surveillance: identification of serotypes 1, 2, and 4 by rt-pcr in Quintana Roo, Mexico |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Detection of aedes aegypti mosquitoes infected with dengue virus as a complementary method for increasing the sensitivity of surveillance: identification of serotypes 1, 2, and 4 by rt-pcr in Quintana Roo, Mexico |
| title_full | Detection of aedes aegypti mosquitoes infected with dengue virus as a complementary method for increasing the sensitivity of surveillance: identification of serotypes 1, 2, and 4 by rt-pcr in Quintana Roo, Mexico |
| title_fullStr | Detection of aedes aegypti mosquitoes infected with dengue virus as a complementary method for increasing the sensitivity of surveillance: identification of serotypes 1, 2, and 4 by rt-pcr in Quintana Roo, Mexico |
| title_full_unstemmed | Detection of aedes aegypti mosquitoes infected with dengue virus as a complementary method for increasing the sensitivity of surveillance: identification of serotypes 1, 2, and 4 by rt-pcr in Quintana Roo, Mexico |
| title_short | Detection of aedes aegypti mosquitoes infected with dengue virus as a complementary method for increasing the sensitivity of surveillance: identification of serotypes 1, 2, and 4 by rt-pcr in Quintana Roo, Mexico |
| title_sort | detection of aedes aegypti mosquitoes infected with dengue virus as a complementary method for increasing the sensitivity of surveillance identification of serotypes 1 2 and 4 by rt pcr in quintana roo mexico |
| topic | Q Ciencias en General QH Historia Natural, Biología |
| url | http://eprints.uanl.mx/6437/1/Detection%20of%20Aedes%20aegypti%20Mosquitoes%20Infected%20with%20Dengue%20Virus%20as%20a%20Complementary%20Method%20for%20Increasing%20the%20Sensitivity%20of%20Surveillance%20Identification%20o.pdf |
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