Efficient secretion of a modified E7 protein from human papilloma virus type-16 by Lactococcus lactis
Aims: To create and provide a strain of the food-grade bacterium Lactococcus lactis able to efficiently secrete a modified form of the E7 protein from the human papilloma virus (HPV) type-16. Methods and Results: We cloned the coding sequence of a modified E7 (E7m)from the HPV-16 in a plasmid regula...
| Autores principales: | , , , , , , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
2010
|
| Materias: | |
| Acceso en línea: | http://eprints.uanl.mx/5409/1/j.1472-765X.2010.02905.x.pdf |
| _version_ | 1824369752342003712 |
|---|---|
| author | Quistián Martínez, Deyanira Villatoro Hernández, Julio Loera Arias, María de Jesús Rangel Colmenero, Blanca Rocío Zavala Flores, Laura Mireya Sepúlveda Saavedra, Julio Guzmán López, Santos Elizondo Omaña, Rodrigo Enrique Montes de Oca Luna, Roberto Saucedo Cárdenas, Odila |
| author_facet | Quistián Martínez, Deyanira Villatoro Hernández, Julio Loera Arias, María de Jesús Rangel Colmenero, Blanca Rocío Zavala Flores, Laura Mireya Sepúlveda Saavedra, Julio Guzmán López, Santos Elizondo Omaña, Rodrigo Enrique Montes de Oca Luna, Roberto Saucedo Cárdenas, Odila |
| author_sort | Quistián Martínez, Deyanira |
| collection | Repositorio Institucional |
| description | Aims: To create and provide a strain of the food-grade bacterium Lactococcus lactis able to efficiently secrete a modified form of the E7 protein from the human papilloma virus (HPV) type-16. Methods and Results: We cloned the coding sequence of a modified E7 (E7m)from the HPV-16 in a plasmid regulated by the strong expression promoter p59. Secretion of the E7m was made by the signal peptide of the usp45 gene.The E7m was detected by Western blot in the cell-free-medium fraction, showing no degradation or aberrant forms.
Conclusions: We constructed a strain of L. lactis able to secrete efficiently aHPV-16 E7 modified protein with diminished transforming activity. Significance and Impact of the Study: Human papilloma virus infection is associated with more than 99% of cervical cancers. Immunotherapy targeting E7 to treat HPV-associated cervical malignancies has been demonstrated to be
highly efficient. However, native E7 maintains transforming activity. We present this new strain of a food-grade bacterium able to efficiently secrete a
HPV-16 E7-modified protein with diminished transforming activity. This new strain could be used as a live vaccine to deliver E7 at a mucosal level and generate antitumour immune responses against HPV-associated tumours. |
| format | Article |
| id | eprints-5409 |
| institution | UANL |
| language | English |
| publishDate | 2010 |
| record_format | eprints |
| spelling | eprints-54092017-01-17T17:12:22Z http://eprints.uanl.mx/5409/ Efficient secretion of a modified E7 protein from human papilloma virus type-16 by Lactococcus lactis Quistián Martínez, Deyanira Villatoro Hernández, Julio Loera Arias, María de Jesús Rangel Colmenero, Blanca Rocío Zavala Flores, Laura Mireya Sepúlveda Saavedra, Julio Guzmán López, Santos Elizondo Omaña, Rodrigo Enrique Montes de Oca Luna, Roberto Saucedo Cárdenas, Odila GV Deportes y Recreación Aims: To create and provide a strain of the food-grade bacterium Lactococcus lactis able to efficiently secrete a modified form of the E7 protein from the human papilloma virus (HPV) type-16. Methods and Results: We cloned the coding sequence of a modified E7 (E7m)from the HPV-16 in a plasmid regulated by the strong expression promoter p59. Secretion of the E7m was made by the signal peptide of the usp45 gene.The E7m was detected by Western blot in the cell-free-medium fraction, showing no degradation or aberrant forms. Conclusions: We constructed a strain of L. lactis able to secrete efficiently aHPV-16 E7 modified protein with diminished transforming activity. Significance and Impact of the Study: Human papilloma virus infection is associated with more than 99% of cervical cancers. Immunotherapy targeting E7 to treat HPV-associated cervical malignancies has been demonstrated to be highly efficient. However, native E7 maintains transforming activity. We present this new strain of a food-grade bacterium able to efficiently secrete a HPV-16 E7-modified protein with diminished transforming activity. This new strain could be used as a live vaccine to deliver E7 at a mucosal level and generate antitumour immune responses against HPV-associated tumours. 2010 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/5409/1/j.1472-765X.2010.02905.x.pdf http://eprints.uanl.mx/5409/1.haspreviewThumbnailVersion/j.1472-765X.2010.02905.x.pdf Quistián Martínez, Deyanira y Villatoro Hernández, Julio y Loera Arias, María de Jesús y Rangel Colmenero, Blanca Rocío y Zavala Flores, Laura Mireya y Sepúlveda Saavedra, Julio y Guzmán López, Santos y Elizondo Omaña, Rodrigo Enrique y Montes de Oca Luna, Roberto y Saucedo Cárdenas, Odila (2010) Efficient secretion of a modified E7 protein from human papilloma virus type-16 by Lactococcus lactis. Letters in applied microbiology, 51. pp. 383-387. ISSN 0266-8254 |
| spellingShingle | GV Deportes y Recreación Quistián Martínez, Deyanira Villatoro Hernández, Julio Loera Arias, María de Jesús Rangel Colmenero, Blanca Rocío Zavala Flores, Laura Mireya Sepúlveda Saavedra, Julio Guzmán López, Santos Elizondo Omaña, Rodrigo Enrique Montes de Oca Luna, Roberto Saucedo Cárdenas, Odila Efficient secretion of a modified E7 protein from human papilloma virus type-16 by Lactococcus lactis |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Efficient secretion of a modified E7 protein from human
papilloma virus type-16 by Lactococcus lactis |
| title_full | Efficient secretion of a modified E7 protein from human
papilloma virus type-16 by Lactococcus lactis |
| title_fullStr | Efficient secretion of a modified E7 protein from human
papilloma virus type-16 by Lactococcus lactis |
| title_full_unstemmed | Efficient secretion of a modified E7 protein from human
papilloma virus type-16 by Lactococcus lactis |
| title_short | Efficient secretion of a modified E7 protein from human
papilloma virus type-16 by Lactococcus lactis |
| title_sort | efficient secretion of a modified e7 protein from human papilloma virus type 16 by lactococcus lactis |
| topic | GV Deportes y Recreación |
| url | http://eprints.uanl.mx/5409/1/j.1472-765X.2010.02905.x.pdf |
| work_keys_str_mv | AT quistianmartinezdeyanira efficientsecretionofamodifiede7proteinfromhumanpapillomavirustype16bylactococcuslactis AT villatorohernandezjulio efficientsecretionofamodifiede7proteinfromhumanpapillomavirustype16bylactococcuslactis AT loeraariasmariadejesus efficientsecretionofamodifiede7proteinfromhumanpapillomavirustype16bylactococcuslactis AT rangelcolmeneroblancarocio efficientsecretionofamodifiede7proteinfromhumanpapillomavirustype16bylactococcuslactis AT zavalafloreslauramireya efficientsecretionofamodifiede7proteinfromhumanpapillomavirustype16bylactococcuslactis AT sepulvedasaavedrajulio efficientsecretionofamodifiede7proteinfromhumanpapillomavirustype16bylactococcuslactis AT guzmanlopezsantos efficientsecretionofamodifiede7proteinfromhumanpapillomavirustype16bylactococcuslactis AT elizondoomanarodrigoenrique efficientsecretionofamodifiede7proteinfromhumanpapillomavirustype16bylactococcuslactis AT montesdeocalunaroberto efficientsecretionofamodifiede7proteinfromhumanpapillomavirustype16bylactococcuslactis AT saucedocardenasodila efficientsecretionofamodifiede7proteinfromhumanpapillomavirustype16bylactococcuslactis |