Evaluation of DNA single and double strand breaks in women with cervical neoplasia based on alkaline and neutral comet assay techniques
A hospital-based unmatched case-control study was performed in order to determine the relation of DNA single (ssb) and double (dsb) strand breaks in women with and without cervical neoplasia. Cervical epithelial cells of 30 women: 10 with low grade squamous intraepithelial lesions (LG-SIL), 10 with...
| Autores principales: | , , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
John Wiley & Sons
2012
|
| Acceso en línea: | http://eprints.uanl.mx/30197/7/30197.pdf |
| _version_ | 1838551029941010432 |
|---|---|
| author | Cortés Gutiérrez, Elva I. Hernández Garza, Fernando García Pérez, Jorge O. Dávila Rodríguez, Martha I. Aguado Barrera, Miguel E. Cerda Flores, Ricardo M. |
| author_facet | Cortés Gutiérrez, Elva I. Hernández Garza, Fernando García Pérez, Jorge O. Dávila Rodríguez, Martha I. Aguado Barrera, Miguel E. Cerda Flores, Ricardo M. |
| author_sort | Cortés Gutiérrez, Elva I. |
| collection | Repositorio Institucional |
| description | A hospital-based unmatched case-control study was performed in order to determine the relation of DNA single (ssb) and double (dsb) strand breaks in women with and without cervical neoplasia. Cervical epithelial cells of 30 women: 10 with low grade squamous intraepithelial lesions (LG-SIL), 10 with high-grade SIL (HG-SIL), and 10 without cervical lesions were evaluated using alkaline and neutral comet assays. A significant increase in global DNA damage (ssb + dsb) and dsb was observed in patients with HG-SIL (48.90 ± 12.87 and 23.50 ± 13.91), patients with LG-SIL (33.60 ± 14.96 and 11.20 ± 5.71), and controls (21.70 ± 11.87 and 5.30 ± 5.38; resp.). Pearson correlation coefficient revealed a strong relation between the levels ssb and dsb (r2 = 0.99, P = 0.03, and r2 = 0.94, P = 0.16, resp.) and progression of neoplasia. The increase of dsb damage in patients with HG-SIL was confirmed by DNA breakage detection-FISH (DBD-FISH) on neutral comets. Our results argue in favor of a real genomic instability in women with cervical neoplasia, which was strengthened by our finding of a higher proportion of DNA dsb. |
| format | Article |
| id | eprints-30197 |
| institution | UANL |
| language | English |
| publishDate | 2012 |
| publisher | John Wiley & Sons |
| record_format | eprints |
| spelling | eprints-301972025-07-07T16:23:06Z http://eprints.uanl.mx/30197/ Evaluation of DNA single and double strand breaks in women with cervical neoplasia based on alkaline and neutral comet assay techniques Cortés Gutiérrez, Elva I. Hernández Garza, Fernando García Pérez, Jorge O. Dávila Rodríguez, Martha I. Aguado Barrera, Miguel E. Cerda Flores, Ricardo M. A hospital-based unmatched case-control study was performed in order to determine the relation of DNA single (ssb) and double (dsb) strand breaks in women with and without cervical neoplasia. Cervical epithelial cells of 30 women: 10 with low grade squamous intraepithelial lesions (LG-SIL), 10 with high-grade SIL (HG-SIL), and 10 without cervical lesions were evaluated using alkaline and neutral comet assays. A significant increase in global DNA damage (ssb + dsb) and dsb was observed in patients with HG-SIL (48.90 ± 12.87 and 23.50 ± 13.91), patients with LG-SIL (33.60 ± 14.96 and 11.20 ± 5.71), and controls (21.70 ± 11.87 and 5.30 ± 5.38; resp.). Pearson correlation coefficient revealed a strong relation between the levels ssb and dsb (r2 = 0.99, P = 0.03, and r2 = 0.94, P = 0.16, resp.) and progression of neoplasia. The increase of dsb damage in patients with HG-SIL was confirmed by DNA breakage detection-FISH (DBD-FISH) on neutral comets. Our results argue in favor of a real genomic instability in women with cervical neoplasia, which was strengthened by our finding of a higher proportion of DNA dsb. John Wiley & Sons 2012-10-03 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/30197/7/30197.pdf http://eprints.uanl.mx/30197/7.haspreviewThumbnailVersion/30197.pdf Cortés Gutiérrez, Elva I. y Hernández Garza, Fernando y García Pérez, Jorge O. y Dávila Rodríguez, Martha I. y Aguado Barrera, Miguel E. y Cerda Flores, Ricardo M. (2012) Evaluation of DNA single and double strand breaks in women with cervical neoplasia based on alkaline and neutral comet assay techniques. Journal of Biomedicine and Biotechnology, 2012. pp. 1-7. ISSN 1110-7243 http://doi.org/10.1155/2012/385245 doi:10.1155/2012/385245 |
| spellingShingle | Cortés Gutiérrez, Elva I. Hernández Garza, Fernando García Pérez, Jorge O. Dávila Rodríguez, Martha I. Aguado Barrera, Miguel E. Cerda Flores, Ricardo M. Evaluation of DNA single and double strand breaks in women with cervical neoplasia based on alkaline and neutral comet assay techniques |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Evaluation of DNA single and double strand breaks in women with cervical neoplasia based on alkaline and neutral comet assay techniques |
| title_full | Evaluation of DNA single and double strand breaks in women with cervical neoplasia based on alkaline and neutral comet assay techniques |
| title_fullStr | Evaluation of DNA single and double strand breaks in women with cervical neoplasia based on alkaline and neutral comet assay techniques |
| title_full_unstemmed | Evaluation of DNA single and double strand breaks in women with cervical neoplasia based on alkaline and neutral comet assay techniques |
| title_short | Evaluation of DNA single and double strand breaks in women with cervical neoplasia based on alkaline and neutral comet assay techniques |
| title_sort | evaluation of dna single and double strand breaks in women with cervical neoplasia based on alkaline and neutral comet assay techniques |
| url | http://eprints.uanl.mx/30197/7/30197.pdf |
| work_keys_str_mv | AT cortesgutierrezelvai evaluationofdnasingleanddoublestrandbreaksinwomenwithcervicalneoplasiabasedonalkalineandneutralcometassaytechniques AT hernandezgarzafernando evaluationofdnasingleanddoublestrandbreaksinwomenwithcervicalneoplasiabasedonalkalineandneutralcometassaytechniques AT garciaperezjorgeo evaluationofdnasingleanddoublestrandbreaksinwomenwithcervicalneoplasiabasedonalkalineandneutralcometassaytechniques AT davilarodriguezmarthai evaluationofdnasingleanddoublestrandbreaksinwomenwithcervicalneoplasiabasedonalkalineandneutralcometassaytechniques AT aguadobarreramiguele evaluationofdnasingleanddoublestrandbreaksinwomenwithcervicalneoplasiabasedonalkalineandneutralcometassaytechniques AT cerdafloresricardom evaluationofdnasingleanddoublestrandbreaksinwomenwithcervicalneoplasiabasedonalkalineandneutralcometassaytechniques |