Anti-giardia activity of hexane extract of <i>Citrus aurantifolia</i> (Christim) swingle and some of its constituents
Background: Giardia lamblia is a parasite that causes giardiasis in humans and other mammals. The common treatment includes different drugs, which were described to produce unpleasant side effects. Citrus aurantifolia, popularly known as “lima”, is a plant used in traditional medicine to treat gast...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
African Journals Online
2015
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| Acceso en línea: | http://eprints.uanl.mx/30185/7/30185.pdf |
| _version_ | 1836640500128415744 |
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| author | Domínguez Vigil, IG Camacho Corona, MdR Heredia Rojas, JA Vargas Villarreal, Javier Rodríguez De la Fuente, AO Heredia Rodríguez, Omar Mata Cárdenas, BD |
| author_facet | Domínguez Vigil, IG Camacho Corona, MdR Heredia Rojas, JA Vargas Villarreal, Javier Rodríguez De la Fuente, AO Heredia Rodríguez, Omar Mata Cárdenas, BD |
| author_sort | Domínguez Vigil, IG |
| collection | Repositorio Institucional |
| description | Background: Giardia lamblia is a parasite that causes giardiasis in humans and other mammals. The common treatment includes different drugs,
which were described to produce unpleasant side effects. Citrus aurantifolia, popularly known as “lima”, is a plant used in traditional medicine to treat gastrointestinal symptoms. The aim of the present study was to evaluate the anti-Giardia activity of 10 pure compounds obtained from a hexanic extract of Mexican lime on the basis of trophozoite growth inhibition.
Materials and Methods: A hexanic extract obtained from fresh fruit peels of Citrus aurantifolia was tested on G. lamblia strain 0989:IMSS
trophozoites cultured in TYI-S-33 medium. The concentration of all standard drugs, analyzed by gas chromatography, was adjusted at 10 mg/mL.
Metronidazole was used as a positive control. Growth inhibition was determined by counting the number of trophozoites using a Neubauer chamber.
The 50% inhibitory concentration (IC50) of each drug was calculated by probit analysis and 95% confidence limits were calculated.
Results: 4-hexen-3-one, citral and geraniol showed IC50 values of 34.2, 64.5 and 229.49 µg/ml in axenic cultures after 24 hr of incubation,
respectively. When these results were compared with a positive control of metronidazole; 4-hexen-3-one was 66 times; citral was 112 and geraniol was 441 times less active respectively. The other tested compounds did not inhibit the growth of cultured G. lamblia trophozoites.
Conclusion: The obtained results lead us to propose that these tested compounds from C. aurantifolia have potential for use as therapeutic agents against giardiasis. |
| format | Article |
| id | eprints-30185 |
| institution | UANL |
| language | English |
| publishDate | 2015 |
| publisher | African Journals Online |
| record_format | eprints |
| spelling | eprints-301852025-07-03T14:33:43Z http://eprints.uanl.mx/30185/ Anti-giardia activity of hexane extract of <i>Citrus aurantifolia</i> (Christim) swingle and some of its constituents Domínguez Vigil, IG Camacho Corona, MdR Heredia Rojas, JA Vargas Villarreal, Javier Rodríguez De la Fuente, AO Heredia Rodríguez, Omar Mata Cárdenas, BD Background: Giardia lamblia is a parasite that causes giardiasis in humans and other mammals. The common treatment includes different drugs, which were described to produce unpleasant side effects. Citrus aurantifolia, popularly known as “lima”, is a plant used in traditional medicine to treat gastrointestinal symptoms. The aim of the present study was to evaluate the anti-Giardia activity of 10 pure compounds obtained from a hexanic extract of Mexican lime on the basis of trophozoite growth inhibition. Materials and Methods: A hexanic extract obtained from fresh fruit peels of Citrus aurantifolia was tested on G. lamblia strain 0989:IMSS trophozoites cultured in TYI-S-33 medium. The concentration of all standard drugs, analyzed by gas chromatography, was adjusted at 10 mg/mL. Metronidazole was used as a positive control. Growth inhibition was determined by counting the number of trophozoites using a Neubauer chamber. The 50% inhibitory concentration (IC50) of each drug was calculated by probit analysis and 95% confidence limits were calculated. Results: 4-hexen-3-one, citral and geraniol showed IC50 values of 34.2, 64.5 and 229.49 µg/ml in axenic cultures after 24 hr of incubation, respectively. When these results were compared with a positive control of metronidazole; 4-hexen-3-one was 66 times; citral was 112 and geraniol was 441 times less active respectively. The other tested compounds did not inhibit the growth of cultured G. lamblia trophozoites. Conclusion: The obtained results lead us to propose that these tested compounds from C. aurantifolia have potential for use as therapeutic agents against giardiasis. African Journals Online 2015 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/30185/7/30185.pdf http://eprints.uanl.mx/30185/7.haspreviewThumbnailVersion/30185.pdf Domínguez Vigil, IG y Camacho Corona, MdR y Heredia Rojas, JA y Vargas Villarreal, Javier y Rodríguez De la Fuente, AO y Heredia Rodríguez, Omar y Mata Cárdenas, BD (2015) Anti-giardia activity of hexane extract of <i>Citrus aurantifolia</i> (Christim) swingle and some of its constituents. African Journal of Traditional, Complementary and Alternative Medicines, 12 (2). pp. 55-59. ISSN 0189-6016 http://doi.org/10.4314/ajtcam.v12i2.10 doi:10.4314/ajtcam.v12i2.10 |
| spellingShingle | Domínguez Vigil, IG Camacho Corona, MdR Heredia Rojas, JA Vargas Villarreal, Javier Rodríguez De la Fuente, AO Heredia Rodríguez, Omar Mata Cárdenas, BD Anti-giardia activity of hexane extract of <i>Citrus aurantifolia</i> (Christim) swingle and some of its constituents |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Anti-giardia activity of hexane extract of <i>Citrus aurantifolia</i> (Christim) swingle and some of its constituents |
| title_full | Anti-giardia activity of hexane extract of <i>Citrus aurantifolia</i> (Christim) swingle and some of its constituents |
| title_fullStr | Anti-giardia activity of hexane extract of <i>Citrus aurantifolia</i> (Christim) swingle and some of its constituents |
| title_full_unstemmed | Anti-giardia activity of hexane extract of <i>Citrus aurantifolia</i> (Christim) swingle and some of its constituents |
| title_short | Anti-giardia activity of hexane extract of <i>Citrus aurantifolia</i> (Christim) swingle and some of its constituents |
| title_sort | anti giardia activity of hexane extract of i citrus aurantifolia i christim swingle and some of its constituents |
| url | http://eprints.uanl.mx/30185/7/30185.pdf |
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