Novel Antennapedia and Ultrabithorax trimeric complexes with TBP and Exd regulate transcription
Background Hox proteins interact with DNA and many other proteins, co-factors, transcriptional factors, chromatin remodeling components, non-coding RNAs and even the extracellular matrix that assembles the Hox complexes. The number of interacting partners continues to grow with diverse components an...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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2024
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| Online Access: | http://eprints.uanl.mx/30123/1/1426.pdf |
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| author | Villarreal Puente, Alely Cecilia Altamirano Torres, Claudia Jiménez Mejía, Gustavo Hernández Bautista, Norma Carolina Montalvo Méndez, Rubén de Jesús Vázquez, Martha Zurita, Mario Reséndez Pérez, Diana |
| author_facet | Villarreal Puente, Alely Cecilia Altamirano Torres, Claudia Jiménez Mejía, Gustavo Hernández Bautista, Norma Carolina Montalvo Méndez, Rubén de Jesús Vázquez, Martha Zurita, Mario Reséndez Pérez, Diana |
| author_sort | Villarreal Puente, Alely Cecilia |
| collection | Repositorio Institucional |
| description | Background Hox proteins interact with DNA and many other proteins, co-factors, transcriptional factors, chromatin remodeling components, non-coding RNAs and even the extracellular matrix that assembles the Hox complexes. The number of interacting partners continues to grow with diverse components and more transcriptional factors than initially thought. Hox complexes present many activities, but their molecular mechanisms to modulate their target genes remain unsolved.
Results In this paper we showed the protein-protein interaction of Antp with Ubx through the homeodomain using BiFC in Drosophila. Analysis of Antp-deletional mutants showed that AntpHD helixes 1 and 2 are required for the interaction with Ubx. Also, we found a novel interaction of Ubx with TBP, in which the PolyQ domain of TBP is required for the interaction. Moreover, we also detected the formation of two new trimeric complexes of Antp with Ubx, TBP and Exd using BiFC-FRET; these proteins, however, do not form a trimeric interaction with BIP2 or TFIIEβ. The novel trimeric complexes reduced Antp transcriptional activity, indicating that they could confer specificity for repression.
Conclusions Our results increase the number of transcriptional factors in the Antp and Ubx interactomes that form two novel trimeric complexes with TBP and Exd. We also report a new Ubx interaction with TBP. These novel interactions provide important clues of the dynamics of Hox-interacting complexes involved in transcriptional regulation, contributing to better understand Hox function. |
| format | Article |
| id | eprints-30123 |
| institution | UANL |
| language | English |
| publishDate | 2024 |
| record_format | eprints |
| spelling | eprints-301232025-07-18T17:17:35Z http://eprints.uanl.mx/30123/ Novel Antennapedia and Ultrabithorax trimeric complexes with TBP and Exd regulate transcription Villarreal Puente, Alely Cecilia Altamirano Torres, Claudia Jiménez Mejía, Gustavo Hernández Bautista, Norma Carolina Montalvo Méndez, Rubén de Jesús Vázquez, Martha Zurita, Mario Reséndez Pérez, Diana QH Historia Natural, Biología Background Hox proteins interact with DNA and many other proteins, co-factors, transcriptional factors, chromatin remodeling components, non-coding RNAs and even the extracellular matrix that assembles the Hox complexes. The number of interacting partners continues to grow with diverse components and more transcriptional factors than initially thought. Hox complexes present many activities, but their molecular mechanisms to modulate their target genes remain unsolved. Results In this paper we showed the protein-protein interaction of Antp with Ubx through the homeodomain using BiFC in Drosophila. Analysis of Antp-deletional mutants showed that AntpHD helixes 1 and 2 are required for the interaction with Ubx. Also, we found a novel interaction of Ubx with TBP, in which the PolyQ domain of TBP is required for the interaction. Moreover, we also detected the formation of two new trimeric complexes of Antp with Ubx, TBP and Exd using BiFC-FRET; these proteins, however, do not form a trimeric interaction with BIP2 or TFIIEβ. The novel trimeric complexes reduced Antp transcriptional activity, indicating that they could confer specificity for repression. Conclusions Our results increase the number of transcriptional factors in the Antp and Ubx interactomes that form two novel trimeric complexes with TBP and Exd. We also report a new Ubx interaction with TBP. These novel interactions provide important clues of the dynamics of Hox-interacting complexes involved in transcriptional regulation, contributing to better understand Hox function. 2024 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/30123/1/1426.pdf http://eprints.uanl.mx/30123/1.haspreviewThumbnailVersion/1426.pdf Villarreal Puente, Alely Cecilia y Altamirano Torres, Claudia y Jiménez Mejía, Gustavo y Hernández Bautista, Norma Carolina y Montalvo Méndez, Rubén de Jesús y Vázquez, Martha y Zurita, Mario y Reséndez Pérez, Diana (2024) Novel Antennapedia and Ultrabithorax trimeric complexes with TBP and Exd regulate transcription. Hereditas, 161 (1). pp. 1-11. ISSN 1601-5223 http://doi.org/10.1186/s41065-024-00327-x doi:10.1186/s41065-024-00327-x |
| spellingShingle | QH Historia Natural, Biología Villarreal Puente, Alely Cecilia Altamirano Torres, Claudia Jiménez Mejía, Gustavo Hernández Bautista, Norma Carolina Montalvo Méndez, Rubén de Jesús Vázquez, Martha Zurita, Mario Reséndez Pérez, Diana Novel Antennapedia and Ultrabithorax trimeric complexes with TBP and Exd regulate transcription |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Novel Antennapedia and Ultrabithorax trimeric complexes with TBP and Exd regulate transcription |
| title_full | Novel Antennapedia and Ultrabithorax trimeric complexes with TBP and Exd regulate transcription |
| title_fullStr | Novel Antennapedia and Ultrabithorax trimeric complexes with TBP and Exd regulate transcription |
| title_full_unstemmed | Novel Antennapedia and Ultrabithorax trimeric complexes with TBP and Exd regulate transcription |
| title_short | Novel Antennapedia and Ultrabithorax trimeric complexes with TBP and Exd regulate transcription |
| title_sort | novel antennapedia and ultrabithorax trimeric complexes with tbp and exd regulate transcription |
| topic | QH Historia Natural, Biología |
| url | http://eprints.uanl.mx/30123/1/1426.pdf |
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