Novel Antennapedia and Ultrabithorax trimeric complexes with TBP and Exd regulate transcription

Background Hox proteins interact with DNA and many other proteins, co-factors, transcriptional factors, chromatin remodeling components, non-coding RNAs and even the extracellular matrix that assembles the Hox complexes. The number of interacting partners continues to grow with diverse components an...

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Autores principales: Villarreal Puente, Alely Cecilia, Altamirano Torres, Claudia, Jiménez Mejía, Gustavo, Hernández Bautista, Norma Carolina, Montalvo Méndez, Rubén de Jesús, Vázquez, Martha, Zurita, Mario, Reséndez Pérez, Diana
Formato: Artículo
Lenguaje:inglés
Publicado: 2024
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Acceso en línea:http://eprints.uanl.mx/30123/1/1426.pdf
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author Villarreal Puente, Alely Cecilia
Altamirano Torres, Claudia
Jiménez Mejía, Gustavo
Hernández Bautista, Norma Carolina
Montalvo Méndez, Rubén de Jesús
Vázquez, Martha
Zurita, Mario
Reséndez Pérez, Diana
author_facet Villarreal Puente, Alely Cecilia
Altamirano Torres, Claudia
Jiménez Mejía, Gustavo
Hernández Bautista, Norma Carolina
Montalvo Méndez, Rubén de Jesús
Vázquez, Martha
Zurita, Mario
Reséndez Pérez, Diana
author_sort Villarreal Puente, Alely Cecilia
collection Repositorio Institucional
description Background Hox proteins interact with DNA and many other proteins, co-factors, transcriptional factors, chromatin remodeling components, non-coding RNAs and even the extracellular matrix that assembles the Hox complexes. The number of interacting partners continues to grow with diverse components and more transcriptional factors than initially thought. Hox complexes present many activities, but their molecular mechanisms to modulate their target genes remain unsolved. Results In this paper we showed the protein-protein interaction of Antp with Ubx through the homeodomain using BiFC in Drosophila. Analysis of Antp-deletional mutants showed that AntpHD helixes 1 and 2 are required for the interaction with Ubx. Also, we found a novel interaction of Ubx with TBP, in which the PolyQ domain of TBP is required for the interaction. Moreover, we also detected the formation of two new trimeric complexes of Antp with Ubx, TBP and Exd using BiFC-FRET; these proteins, however, do not form a trimeric interaction with BIP2 or TFIIEβ. The novel trimeric complexes reduced Antp transcriptional activity, indicating that they could confer specificity for repression. Conclusions Our results increase the number of transcriptional factors in the Antp and Ubx interactomes that form two novel trimeric complexes with TBP and Exd. We also report a new Ubx interaction with TBP. These novel interactions provide important clues of the dynamics of Hox-interacting complexes involved in transcriptional regulation, contributing to better understand Hox function.
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spelling eprints-301232025-07-18T17:17:35Z http://eprints.uanl.mx/30123/ Novel Antennapedia and Ultrabithorax trimeric complexes with TBP and Exd regulate transcription Villarreal Puente, Alely Cecilia Altamirano Torres, Claudia Jiménez Mejía, Gustavo Hernández Bautista, Norma Carolina Montalvo Méndez, Rubén de Jesús Vázquez, Martha Zurita, Mario Reséndez Pérez, Diana QH Historia Natural, Biología Background Hox proteins interact with DNA and many other proteins, co-factors, transcriptional factors, chromatin remodeling components, non-coding RNAs and even the extracellular matrix that assembles the Hox complexes. The number of interacting partners continues to grow with diverse components and more transcriptional factors than initially thought. Hox complexes present many activities, but their molecular mechanisms to modulate their target genes remain unsolved. Results In this paper we showed the protein-protein interaction of Antp with Ubx through the homeodomain using BiFC in Drosophila. Analysis of Antp-deletional mutants showed that AntpHD helixes 1 and 2 are required for the interaction with Ubx. Also, we found a novel interaction of Ubx with TBP, in which the PolyQ domain of TBP is required for the interaction. Moreover, we also detected the formation of two new trimeric complexes of Antp with Ubx, TBP and Exd using BiFC-FRET; these proteins, however, do not form a trimeric interaction with BIP2 or TFIIEβ. The novel trimeric complexes reduced Antp transcriptional activity, indicating that they could confer specificity for repression. Conclusions Our results increase the number of transcriptional factors in the Antp and Ubx interactomes that form two novel trimeric complexes with TBP and Exd. We also report a new Ubx interaction with TBP. These novel interactions provide important clues of the dynamics of Hox-interacting complexes involved in transcriptional regulation, contributing to better understand Hox function. 2024 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/30123/1/1426.pdf http://eprints.uanl.mx/30123/1.haspreviewThumbnailVersion/1426.pdf Villarreal Puente, Alely Cecilia y Altamirano Torres, Claudia y Jiménez Mejía, Gustavo y Hernández Bautista, Norma Carolina y Montalvo Méndez, Rubén de Jesús y Vázquez, Martha y Zurita, Mario y Reséndez Pérez, Diana (2024) Novel Antennapedia and Ultrabithorax trimeric complexes with TBP and Exd regulate transcription. Hereditas, 161 (1). pp. 1-11. ISSN 1601-5223 http://doi.org/10.1186/s41065-024-00327-x doi:10.1186/s41065-024-00327-x
spellingShingle QH Historia Natural, Biología
Villarreal Puente, Alely Cecilia
Altamirano Torres, Claudia
Jiménez Mejía, Gustavo
Hernández Bautista, Norma Carolina
Montalvo Méndez, Rubén de Jesús
Vázquez, Martha
Zurita, Mario
Reséndez Pérez, Diana
Novel Antennapedia and Ultrabithorax trimeric complexes with TBP and Exd regulate transcription
thumbnail https://rediab.uanl.mx/themes/sandal5/images/online.png
title Novel Antennapedia and Ultrabithorax trimeric complexes with TBP and Exd regulate transcription
title_full Novel Antennapedia and Ultrabithorax trimeric complexes with TBP and Exd regulate transcription
title_fullStr Novel Antennapedia and Ultrabithorax trimeric complexes with TBP and Exd regulate transcription
title_full_unstemmed Novel Antennapedia and Ultrabithorax trimeric complexes with TBP and Exd regulate transcription
title_short Novel Antennapedia and Ultrabithorax trimeric complexes with TBP and Exd regulate transcription
title_sort novel antennapedia and ultrabithorax trimeric complexes with tbp and exd regulate transcription
topic QH Historia Natural, Biología
url http://eprints.uanl.mx/30123/1/1426.pdf
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