Immunoinformatics approach to design a novel epitope-based oral vaccine against helicobacter pylori
Helicobacter pylori is an infectious agent that colonizes the gastric mucosa of half of the population worldwide. This bacterium has been recognized as belonging to group 1 carcinogen by the World Health Organization for the role in development of gastritis, peptic ulcers, and cancer. Due to the inc...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Sage Publications
2019
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| Online Access: | http://eprints.uanl.mx/29709/7/29709.pdf |
| _version_ | 1838551020932694016 |
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| author | Urrutia Baca, Víctor Hugo Gomez Flores, Ricardo De la Garza Ramos, Myriam Angélica Tamez Guerra, Patricia Lucio Sauceda, Daniela Guadalupe Rodríguez Padilla, María Cristina |
| author_facet | Urrutia Baca, Víctor Hugo Gomez Flores, Ricardo De la Garza Ramos, Myriam Angélica Tamez Guerra, Patricia Lucio Sauceda, Daniela Guadalupe Rodríguez Padilla, María Cristina |
| author_sort | Urrutia Baca, Víctor Hugo |
| collection | Repositorio Institucional |
| description | Helicobacter pylori is an infectious agent that colonizes the gastric mucosa of half of the population worldwide. This bacterium has been recognized as belonging to group 1 carcinogen by the World Health Organization for the role in development of gastritis, peptic ulcers, and cancer. Due to the increase in resistance to antibiotics used in the anti-H. pylori therapy, the development of an effective vaccine is an alternative of great interest, which remains a challenge. Therefore, a rational, strategic, and efficient vaccine design against H. pylori is necessary where the use of the most current bioinformatics tools could help achieve it. In this study, immunoinformatics approach was used to design a novel multi epitope oral vaccine against H. pylori. Our multi epitope vaccine is composed of cholera toxin subunit B (CTB) that is used as a mucosal adjuvant to enhance vaccine immunogenicity for oral immunization. CTB fused to 11 epitopes predicted of pathogenic (UreB170–189, VacA459–478, CagA1103–1122, GGT106–126, NapA30–44, and OipA211–230) and colonization (HpaA33–52, FlaA487–506, FecA437–456, BabA129–149, and SabA540–559) proteins from H. pylori. CKS9 peptide (CKSTHPLSC) targets epithelial microfold cells to enhance vaccine uptake from the gut barrier. All sequences were joined to each other by proper linkers. The vaccine was modeled and validated to achieve a high-quality three-dimensional structure. The vaccine design was evaluated as non allergenic, antigenic, soluble, and with an appropriate molecular weight and isoelectric point. Our results suggest that our newly designed vaccine could serve as a promising anti-H. pylori vaccine candidate. |
| format | Article |
| id | eprints-29709 |
| institution | UANL |
| language | English |
| publishDate | 2019 |
| publisher | Sage Publications |
| record_format | eprints |
| spelling | eprints-297092025-07-14T16:10:05Z http://eprints.uanl.mx/29709/ Immunoinformatics approach to design a novel epitope-based oral vaccine against helicobacter pylori Urrutia Baca, Víctor Hugo Gomez Flores, Ricardo De la Garza Ramos, Myriam Angélica Tamez Guerra, Patricia Lucio Sauceda, Daniela Guadalupe Rodríguez Padilla, María Cristina Helicobacter pylori is an infectious agent that colonizes the gastric mucosa of half of the population worldwide. This bacterium has been recognized as belonging to group 1 carcinogen by the World Health Organization for the role in development of gastritis, peptic ulcers, and cancer. Due to the increase in resistance to antibiotics used in the anti-H. pylori therapy, the development of an effective vaccine is an alternative of great interest, which remains a challenge. Therefore, a rational, strategic, and efficient vaccine design against H. pylori is necessary where the use of the most current bioinformatics tools could help achieve it. In this study, immunoinformatics approach was used to design a novel multi epitope oral vaccine against H. pylori. Our multi epitope vaccine is composed of cholera toxin subunit B (CTB) that is used as a mucosal adjuvant to enhance vaccine immunogenicity for oral immunization. CTB fused to 11 epitopes predicted of pathogenic (UreB170–189, VacA459–478, CagA1103–1122, GGT106–126, NapA30–44, and OipA211–230) and colonization (HpaA33–52, FlaA487–506, FecA437–456, BabA129–149, and SabA540–559) proteins from H. pylori. CKS9 peptide (CKSTHPLSC) targets epithelial microfold cells to enhance vaccine uptake from the gut barrier. All sequences were joined to each other by proper linkers. The vaccine was modeled and validated to achieve a high-quality three-dimensional structure. The vaccine design was evaluated as non allergenic, antigenic, soluble, and with an appropriate molecular weight and isoelectric point. Our results suggest that our newly designed vaccine could serve as a promising anti-H. pylori vaccine candidate. Sage Publications 2019 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/29709/7/29709.pdf http://eprints.uanl.mx/29709/7.haspreviewThumbnailVersion/29709.pdf Urrutia Baca, Víctor Hugo y Gomez Flores, Ricardo y De la Garza Ramos, Myriam Angélica y Tamez Guerra, Patricia y Lucio Sauceda, Daniela Guadalupe y Rodríguez Padilla, María Cristina (2019) Immunoinformatics approach to design a novel epitope-based oral vaccine against helicobacter pylori. Journal of Computational Biology, 26 (10). pp. 1177-1190. ISSN 1557-8666 doi:10.1089/cmb.2019.0062 |
| spellingShingle | Urrutia Baca, Víctor Hugo Gomez Flores, Ricardo De la Garza Ramos, Myriam Angélica Tamez Guerra, Patricia Lucio Sauceda, Daniela Guadalupe Rodríguez Padilla, María Cristina Immunoinformatics approach to design a novel epitope-based oral vaccine against helicobacter pylori |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Immunoinformatics approach to design a novel epitope-based oral vaccine against helicobacter pylori |
| title_full | Immunoinformatics approach to design a novel epitope-based oral vaccine against helicobacter pylori |
| title_fullStr | Immunoinformatics approach to design a novel epitope-based oral vaccine against helicobacter pylori |
| title_full_unstemmed | Immunoinformatics approach to design a novel epitope-based oral vaccine against helicobacter pylori |
| title_short | Immunoinformatics approach to design a novel epitope-based oral vaccine against helicobacter pylori |
| title_sort | immunoinformatics approach to design a novel epitope based oral vaccine against helicobacter pylori |
| url | http://eprints.uanl.mx/29709/7/29709.pdf |
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