Expression of Foxp3, CD25 and IL-2 in the B16F10 cancer cell line and melanoma is correlated with tumor growth in mice

The forkhead box P3 (Foxp3) transcription factor is one of the most studied markers used to identify CD4+CD25+ regulatory T cells (Tregs), and has been identified as a key regulator in the development and function of Tregs. Foxp3 expression has been reported in a variety of solid human tumors, inclu...

Descripción completa

Detalles Bibliográficos
Autores principales: Miranda Hernández, D.F., Franco Molina, M.A., Mendoza Gamboa, E., Zapata Benavides, P., Sierra Rivera, C.A., Coronado Cerda, E.E., Rosas Taraco, A.G., Taméz Guerra, R.S., Rodríguez Padilla, C.
Formato: Artículo
Lenguaje:inglés
Publicado: Spandidos Publications 2013
Acceso en línea:http://eprints.uanl.mx/29652/1/1199.pdf
_version_ 1838551019398627328
author Miranda Hernández, D.F.
Franco Molina, M.A.
Mendoza Gamboa, E.
Zapata Benavides, P.
Sierra Rivera, C.A.
Coronado Cerda, E.E.
Rosas Taraco, A.G.
Taméz Guerra, R.S.
Rodríguez Padilla, C.
author_facet Miranda Hernández, D.F.
Franco Molina, M.A.
Mendoza Gamboa, E.
Zapata Benavides, P.
Sierra Rivera, C.A.
Coronado Cerda, E.E.
Rosas Taraco, A.G.
Taméz Guerra, R.S.
Rodríguez Padilla, C.
author_sort Miranda Hernández, D.F.
collection Repositorio Institucional
description The forkhead box P3 (Foxp3) transcription factor is one of the most studied markers used to identify CD4+CD25+ regulatory T cells (Tregs), and has been identified as a key regulator in the development and function of Tregs. Foxp3 expression has been reported in a variety of solid human tumors, including melanoma. The aims of the present study were to analyze Foxp3 expression in B16F10 melanoma cells in vitro, to determine whether this expression was affected during tumor growth in a murine melanoma model and to correlate Foxp3 expression with CD25 expression, interleukin (IL)-2 production and tumor weight. Foxp3 expression was analyzed with quantitative (q)PCR, flow cytometry and confocal microscopy. CD25 expression was analyzed by flow cytometry, and cytokine production was measured by ELISA [IL-2, interferon (IFN)-γ, transforming growth factor (TGF)-β and IL-10] and flow cytometry (IL-2, IFN-γ, IL-4 and IL-5). Foxp3 and CD25 expression was detected in the B16F10 cells in culture and in the intratumoral B16F10 cells. An increase in Foxp3 and CD25 expression was observed in a time-dependent manner during tumor growth at 7, 14 and 21 days. The production of the IL-2, IL-10, IFN-γ and TGF-β cytokines was observed in the B16F10 cells and also detected in the tumoral microenvironment during tumor growth (7, 14 and 21 days). An increase in IL-2 and IL-10 production was observed, whereas IFN-γ production decreased in a time-dependent manner. The production of tumor necrosis factor (TNF)-α was not observed in culture, but was detected during tumor growth, whereas the production of IL-4 and IL-5 was not detected. These data showed a positive correlation between the expression of Foxp3, CD25 and IL-2 and tumor weight in murine melanoma. From these data, it may be suggested that Foxp3 participates in melanoma growth, the modulation of the IL-2, IFN-γ and TNF-α cytokines and CD25 expression, and that it also plays a possible role in immunosuppression.
format Article
id eprints-29652
institution UANL
language English
publishDate 2013
publisher Spandidos Publications
record_format eprints
spelling eprints-296522025-07-16T16:32:43Z http://eprints.uanl.mx/29652/ Expression of Foxp3, CD25 and IL-2 in the B16F10 cancer cell line and melanoma is correlated with tumor growth in mice Miranda Hernández, D.F. Franco Molina, M.A. Mendoza Gamboa, E. Zapata Benavides, P. Sierra Rivera, C.A. Coronado Cerda, E.E. Rosas Taraco, A.G. Taméz Guerra, R.S. Rodríguez Padilla, C. The forkhead box P3 (Foxp3) transcription factor is one of the most studied markers used to identify CD4+CD25+ regulatory T cells (Tregs), and has been identified as a key regulator in the development and function of Tregs. Foxp3 expression has been reported in a variety of solid human tumors, including melanoma. The aims of the present study were to analyze Foxp3 expression in B16F10 melanoma cells in vitro, to determine whether this expression was affected during tumor growth in a murine melanoma model and to correlate Foxp3 expression with CD25 expression, interleukin (IL)-2 production and tumor weight. Foxp3 expression was analyzed with quantitative (q)PCR, flow cytometry and confocal microscopy. CD25 expression was analyzed by flow cytometry, and cytokine production was measured by ELISA [IL-2, interferon (IFN)-γ, transforming growth factor (TGF)-β and IL-10] and flow cytometry (IL-2, IFN-γ, IL-4 and IL-5). Foxp3 and CD25 expression was detected in the B16F10 cells in culture and in the intratumoral B16F10 cells. An increase in Foxp3 and CD25 expression was observed in a time-dependent manner during tumor growth at 7, 14 and 21 days. The production of the IL-2, IL-10, IFN-γ and TGF-β cytokines was observed in the B16F10 cells and also detected in the tumoral microenvironment during tumor growth (7, 14 and 21 days). An increase in IL-2 and IL-10 production was observed, whereas IFN-γ production decreased in a time-dependent manner. The production of tumor necrosis factor (TNF)-α was not observed in culture, but was detected during tumor growth, whereas the production of IL-4 and IL-5 was not detected. These data showed a positive correlation between the expression of Foxp3, CD25 and IL-2 and tumor weight in murine melanoma. From these data, it may be suggested that Foxp3 participates in melanoma growth, the modulation of the IL-2, IFN-γ and TNF-α cytokines and CD25 expression, and that it also plays a possible role in immunosuppression. Spandidos Publications 2013-11 Article PeerReviewed text en http://eprints.uanl.mx/29652/1/1199.pdf http://eprints.uanl.mx/29652/1.haspreviewThumbnailVersion/1199.pdf Miranda Hernández, D.F. y Franco Molina, M.A. y Mendoza Gamboa, E. y Zapata Benavides, P. y Sierra Rivera, C.A. y Coronado Cerda, E.E. y Rosas Taraco, A.G. y Taméz Guerra, R.S. y Rodríguez Padilla, C. (2013) Expression of Foxp3, CD25 and IL-2 in the B16F10 cancer cell line and melanoma is correlated with tumor growth in mice. Oncology Letters, 6 (5). pp. 1195-1200. ISSN 1792-1074 doi:10.3892/ol.2013.1526
spellingShingle Miranda Hernández, D.F.
Franco Molina, M.A.
Mendoza Gamboa, E.
Zapata Benavides, P.
Sierra Rivera, C.A.
Coronado Cerda, E.E.
Rosas Taraco, A.G.
Taméz Guerra, R.S.
Rodríguez Padilla, C.
Expression of Foxp3, CD25 and IL-2 in the B16F10 cancer cell line and melanoma is correlated with tumor growth in mice
thumbnail https://rediab.uanl.mx/themes/sandal5/images/online.png
title Expression of Foxp3, CD25 and IL-2 in the B16F10 cancer cell line and melanoma is correlated with tumor growth in mice
title_full Expression of Foxp3, CD25 and IL-2 in the B16F10 cancer cell line and melanoma is correlated with tumor growth in mice
title_fullStr Expression of Foxp3, CD25 and IL-2 in the B16F10 cancer cell line and melanoma is correlated with tumor growth in mice
title_full_unstemmed Expression of Foxp3, CD25 and IL-2 in the B16F10 cancer cell line and melanoma is correlated with tumor growth in mice
title_short Expression of Foxp3, CD25 and IL-2 in the B16F10 cancer cell line and melanoma is correlated with tumor growth in mice
title_sort expression of foxp3 cd25 and il 2 in the b16f10 cancer cell line and melanoma is correlated with tumor growth in mice
url http://eprints.uanl.mx/29652/1/1199.pdf
work_keys_str_mv AT mirandahernandezdf expressionoffoxp3cd25andil2intheb16f10cancercelllineandmelanomaiscorrelatedwithtumorgrowthinmice
AT francomolinama expressionoffoxp3cd25andil2intheb16f10cancercelllineandmelanomaiscorrelatedwithtumorgrowthinmice
AT mendozagamboae expressionoffoxp3cd25andil2intheb16f10cancercelllineandmelanomaiscorrelatedwithtumorgrowthinmice
AT zapatabenavidesp expressionoffoxp3cd25andil2intheb16f10cancercelllineandmelanomaiscorrelatedwithtumorgrowthinmice
AT sierrariveraca expressionoffoxp3cd25andil2intheb16f10cancercelllineandmelanomaiscorrelatedwithtumorgrowthinmice
AT coronadocerdaee expressionoffoxp3cd25andil2intheb16f10cancercelllineandmelanomaiscorrelatedwithtumorgrowthinmice
AT rosastaracoag expressionoffoxp3cd25andil2intheb16f10cancercelllineandmelanomaiscorrelatedwithtumorgrowthinmice
AT tamezguerrars expressionoffoxp3cd25andil2intheb16f10cancercelllineandmelanomaiscorrelatedwithtumorgrowthinmice
AT rodriguezpadillac expressionoffoxp3cd25andil2intheb16f10cancercelllineandmelanomaiscorrelatedwithtumorgrowthinmice