In Planta Detection of Beauveria bassiana (Ascomycota: Hypocreales) Strains as Endophytes in Bean (Phaseolus vulgaris L.)
Beauveria bassiana (B. bassiana) is a significant entomopathogenic fungus (EPF) in agricultureas a sprayable biocontrol agent. It has the potential to be established as an endophyte (ENP) in various crops, resulting in beneficial effects for the host plants, including resistance to pest insects and...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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2023
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| Online Access: | http://eprints.uanl.mx/29154/1/1049.pdf |
| _version_ | 1842097097360277504 |
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| author | Cavazos Vallejo, Teodora Valadéz Lira, José Alberto Orozco Flores, Alonso A. Gómez Flores, Ricardo Alberto Ek Ramos, María Julissa Quistián Martínez, Deyanira Alcocer González, Juan Manuel Tamez Guerra, Patricia |
| author_facet | Cavazos Vallejo, Teodora Valadéz Lira, José Alberto Orozco Flores, Alonso A. Gómez Flores, Ricardo Alberto Ek Ramos, María Julissa Quistián Martínez, Deyanira Alcocer González, Juan Manuel Tamez Guerra, Patricia |
| author_sort | Cavazos Vallejo, Teodora |
| collection | Repositorio Institucional |
| description | Beauveria bassiana (B. bassiana) is a significant entomopathogenic fungus (EPF) in agricultureas a sprayable biocontrol agent. It has the potential to be established as an endophyte (ENP) in various crops, resulting in beneficial effects for the host plants, including resistance to pest insects and increased growth and yield. However, it is not known whether a B. bassiana strain has such a favorable impact on the plant, since it is a common soil microorganism. Therefore, techniques that allow strain monitoring will be advantageous. To date, methods for detecting or monitoring a specific EPF strain after external application are scarce. In the present study, an in planta nested PCR technique was standardized to differentiate between three B. bassiana strains (GHA, PTG4, and BB37) established as endophytes in bean plants under laboratory conditions by detecting the insertion profile of four group I introns located in the 28S gene of B. bassiana ribosomal DNA. This technique recognized a distinct pattern of bands of different sizes for each strain, with a sensitivity of 1 pg per 10 ng of plant DNA. This molecular approach may be more effective monitoring B. bassiana strains after application to evaluate their significance on crops. |
| format | Article |
| id | eprints-29154 |
| institution | UANL |
| language | English |
| publishDate | 2023 |
| record_format | eprints |
| spelling | eprints-291542025-08-28T14:27:53Z http://eprints.uanl.mx/29154/ In Planta Detection of Beauveria bassiana (Ascomycota: Hypocreales) Strains as Endophytes in Bean (Phaseolus vulgaris L.) Cavazos Vallejo, Teodora Valadéz Lira, José Alberto Orozco Flores, Alonso A. Gómez Flores, Ricardo Alberto Ek Ramos, María Julissa Quistián Martínez, Deyanira Alcocer González, Juan Manuel Tamez Guerra, Patricia QR Microbiología Beauveria bassiana (B. bassiana) is a significant entomopathogenic fungus (EPF) in agricultureas a sprayable biocontrol agent. It has the potential to be established as an endophyte (ENP) in various crops, resulting in beneficial effects for the host plants, including resistance to pest insects and increased growth and yield. However, it is not known whether a B. bassiana strain has such a favorable impact on the plant, since it is a common soil microorganism. Therefore, techniques that allow strain monitoring will be advantageous. To date, methods for detecting or monitoring a specific EPF strain after external application are scarce. In the present study, an in planta nested PCR technique was standardized to differentiate between three B. bassiana strains (GHA, PTG4, and BB37) established as endophytes in bean plants under laboratory conditions by detecting the insertion profile of four group I introns located in the 28S gene of B. bassiana ribosomal DNA. This technique recognized a distinct pattern of bands of different sizes for each strain, with a sensitivity of 1 pg per 10 ng of plant DNA. This molecular approach may be more effective monitoring B. bassiana strains after application to evaluate their significance on crops. 2023 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/29154/1/1049.pdf http://eprints.uanl.mx/29154/1.haspreviewThumbnailVersion/1049.pdf Cavazos Vallejo, Teodora y Valadéz Lira, José Alberto y Orozco Flores, Alonso A. y Gómez Flores, Ricardo Alberto y Ek Ramos, María Julissa y Quistián Martínez, Deyanira y Alcocer González, Juan Manuel y Tamez Guerra, Patricia (2023) In Planta Detection of Beauveria bassiana (Ascomycota: Hypocreales) Strains as Endophytes in Bean (Phaseolus vulgaris L.). Plants, 13 (1). pp. 1-17. ISSN 2223-7747 http://doi.org/10.3390/plants13010022 doi:10.3390/plants13010022 |
| spellingShingle | QR Microbiología Cavazos Vallejo, Teodora Valadéz Lira, José Alberto Orozco Flores, Alonso A. Gómez Flores, Ricardo Alberto Ek Ramos, María Julissa Quistián Martínez, Deyanira Alcocer González, Juan Manuel Tamez Guerra, Patricia In Planta Detection of Beauveria bassiana (Ascomycota: Hypocreales) Strains as Endophytes in Bean (Phaseolus vulgaris L.) |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | In Planta Detection of Beauveria bassiana (Ascomycota: Hypocreales) Strains as Endophytes in Bean (Phaseolus vulgaris L.) |
| title_full | In Planta Detection of Beauveria bassiana (Ascomycota: Hypocreales) Strains as Endophytes in Bean (Phaseolus vulgaris L.) |
| title_fullStr | In Planta Detection of Beauveria bassiana (Ascomycota: Hypocreales) Strains as Endophytes in Bean (Phaseolus vulgaris L.) |
| title_full_unstemmed | In Planta Detection of Beauveria bassiana (Ascomycota: Hypocreales) Strains as Endophytes in Bean (Phaseolus vulgaris L.) |
| title_short | In Planta Detection of Beauveria bassiana (Ascomycota: Hypocreales) Strains as Endophytes in Bean (Phaseolus vulgaris L.) |
| title_sort | in planta detection of beauveria bassiana ascomycota hypocreales strains as endophytes in bean phaseolus vulgaris l |
| topic | QR Microbiología |
| url | http://eprints.uanl.mx/29154/1/1049.pdf |
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