In Planta Detection of Beauveria bassiana (Ascomycota: Hypocreales) Strains as Endophytes in Bean (Phaseolus vulgaris L.)

Beauveria bassiana (B. bassiana) is a significant entomopathogenic fungus (EPF) in agricultureas a sprayable biocontrol agent. It has the potential to be established as an endophyte (ENP) in various crops, resulting in beneficial effects for the host plants, including resistance to pest insects and...

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Autores principales: Cavazos Vallejo, Teodora, Valadéz Lira, José Alberto, Orozco Flores, Alonso A., Gómez Flores, Ricardo Alberto, Ek Ramos, María Julissa, Quistián Martínez, Deyanira, Alcocer González, Juan Manuel, Tamez Guerra, Patricia
Formato: Artículo
Lenguaje:inglés
Publicado: 2023
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Acceso en línea:http://eprints.uanl.mx/29154/1/1049.pdf
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author Cavazos Vallejo, Teodora
Valadéz Lira, José Alberto
Orozco Flores, Alonso A.
Gómez Flores, Ricardo Alberto
Ek Ramos, María Julissa
Quistián Martínez, Deyanira
Alcocer González, Juan Manuel
Tamez Guerra, Patricia
author_facet Cavazos Vallejo, Teodora
Valadéz Lira, José Alberto
Orozco Flores, Alonso A.
Gómez Flores, Ricardo Alberto
Ek Ramos, María Julissa
Quistián Martínez, Deyanira
Alcocer González, Juan Manuel
Tamez Guerra, Patricia
author_sort Cavazos Vallejo, Teodora
collection Repositorio Institucional
description Beauveria bassiana (B. bassiana) is a significant entomopathogenic fungus (EPF) in agricultureas a sprayable biocontrol agent. It has the potential to be established as an endophyte (ENP) in various crops, resulting in beneficial effects for the host plants, including resistance to pest insects and increased growth and yield. However, it is not known whether a B. bassiana strain has such a favorable impact on the plant, since it is a common soil microorganism. Therefore, techniques that allow strain monitoring will be advantageous. To date, methods for detecting or monitoring a specific EPF strain after external application are scarce. In the present study, an in planta nested PCR technique was standardized to differentiate between three B. bassiana strains (GHA, PTG4, and BB37) established as endophytes in bean plants under laboratory conditions by detecting the insertion profile of four group I introns located in the 28S gene of B. bassiana ribosomal DNA. This technique recognized a distinct pattern of bands of different sizes for each strain, with a sensitivity of 1 pg per 10 ng of plant DNA. This molecular approach may be more effective monitoring B. bassiana strains after application to evaluate their significance on crops.
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spelling eprints-291542025-08-28T14:27:53Z http://eprints.uanl.mx/29154/ In Planta Detection of Beauveria bassiana (Ascomycota: Hypocreales) Strains as Endophytes in Bean (Phaseolus vulgaris L.) Cavazos Vallejo, Teodora Valadéz Lira, José Alberto Orozco Flores, Alonso A. Gómez Flores, Ricardo Alberto Ek Ramos, María Julissa Quistián Martínez, Deyanira Alcocer González, Juan Manuel Tamez Guerra, Patricia QR Microbiología Beauveria bassiana (B. bassiana) is a significant entomopathogenic fungus (EPF) in agricultureas a sprayable biocontrol agent. It has the potential to be established as an endophyte (ENP) in various crops, resulting in beneficial effects for the host plants, including resistance to pest insects and increased growth and yield. However, it is not known whether a B. bassiana strain has such a favorable impact on the plant, since it is a common soil microorganism. Therefore, techniques that allow strain monitoring will be advantageous. To date, methods for detecting or monitoring a specific EPF strain after external application are scarce. In the present study, an in planta nested PCR technique was standardized to differentiate between three B. bassiana strains (GHA, PTG4, and BB37) established as endophytes in bean plants under laboratory conditions by detecting the insertion profile of four group I introns located in the 28S gene of B. bassiana ribosomal DNA. This technique recognized a distinct pattern of bands of different sizes for each strain, with a sensitivity of 1 pg per 10 ng of plant DNA. This molecular approach may be more effective monitoring B. bassiana strains after application to evaluate their significance on crops. 2023 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/29154/1/1049.pdf http://eprints.uanl.mx/29154/1.haspreviewThumbnailVersion/1049.pdf Cavazos Vallejo, Teodora y Valadéz Lira, José Alberto y Orozco Flores, Alonso A. y Gómez Flores, Ricardo Alberto y Ek Ramos, María Julissa y Quistián Martínez, Deyanira y Alcocer González, Juan Manuel y Tamez Guerra, Patricia (2023) In Planta Detection of Beauveria bassiana (Ascomycota: Hypocreales) Strains as Endophytes in Bean (Phaseolus vulgaris L.). Plants, 13 (1). pp. 1-17. ISSN 2223-7747 http://doi.org/10.3390/plants13010022 doi:10.3390/plants13010022
spellingShingle QR Microbiología
Cavazos Vallejo, Teodora
Valadéz Lira, José Alberto
Orozco Flores, Alonso A.
Gómez Flores, Ricardo Alberto
Ek Ramos, María Julissa
Quistián Martínez, Deyanira
Alcocer González, Juan Manuel
Tamez Guerra, Patricia
In Planta Detection of Beauveria bassiana (Ascomycota: Hypocreales) Strains as Endophytes in Bean (Phaseolus vulgaris L.)
thumbnail https://rediab.uanl.mx/themes/sandal5/images/online.png
title In Planta Detection of Beauveria bassiana (Ascomycota: Hypocreales) Strains as Endophytes in Bean (Phaseolus vulgaris L.)
title_full In Planta Detection of Beauveria bassiana (Ascomycota: Hypocreales) Strains as Endophytes in Bean (Phaseolus vulgaris L.)
title_fullStr In Planta Detection of Beauveria bassiana (Ascomycota: Hypocreales) Strains as Endophytes in Bean (Phaseolus vulgaris L.)
title_full_unstemmed In Planta Detection of Beauveria bassiana (Ascomycota: Hypocreales) Strains as Endophytes in Bean (Phaseolus vulgaris L.)
title_short In Planta Detection of Beauveria bassiana (Ascomycota: Hypocreales) Strains as Endophytes in Bean (Phaseolus vulgaris L.)
title_sort in planta detection of beauveria bassiana ascomycota hypocreales strains as endophytes in bean phaseolus vulgaris l
topic QR Microbiología
url http://eprints.uanl.mx/29154/1/1049.pdf
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