Genotyping of recombinant Pichia pastoris strains
A simplified amplified-fragment length polymorphism (AFLP) method was used to genotype Pichia pastoris strains obtained by transformation of P. pastoris strain GS115 with a single integration vector. A total of 14 transformants and 3 control strains were analyzed, which generated 16 different band...
| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
BioMed Central
2006
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| Materias: | |
| Acceso en línea: | http://eprints.uanl.mx/27213/1/217.pdf |
| _version_ | 1824420687419277312 |
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| author | Viader Salvadó, José María Cab Barrera, Eddy Luz Galán Wong, Luis Jesús Guerrero Olazarán, Martha |
| author_facet | Viader Salvadó, José María Cab Barrera, Eddy Luz Galán Wong, Luis Jesús Guerrero Olazarán, Martha |
| author_sort | Viader Salvadó, José María |
| collection | Repositorio Institucional |
| description | A simplified amplified-fragment length polymorphism (AFLP) method was used to genotype Pichia pastoris strains obtained by transformation
of P. pastoris strain GS115 with a single integration vector. A total of 14 transformants and 3 control strains were analyzed, which generated 16 different band patterns. A clonal variation was obtained after the transformation process due to genetic differences generated during the transformation event of the host strain. Furthermore, the cluster analysis showed that the transformants with lesser genetic differences with respect to the P. pastoris host strain are the recombinant strains with the highest level of recombinant protein production. |
| format | Article |
| id | eprints-27213 |
| institution | UANL |
| language | English |
| publishDate | 2006 |
| publisher | BioMed Central |
| record_format | eprints |
| spelling | eprints-272132024-04-23T14:28:58Z http://eprints.uanl.mx/27213/ Genotyping of recombinant Pichia pastoris strains Viader Salvadó, José María Cab Barrera, Eddy Luz Galán Wong, Luis Jesús Guerrero Olazarán, Martha QR Microbiología A simplified amplified-fragment length polymorphism (AFLP) method was used to genotype Pichia pastoris strains obtained by transformation of P. pastoris strain GS115 with a single integration vector. A total of 14 transformants and 3 control strains were analyzed, which generated 16 different band patterns. A clonal variation was obtained after the transformation process due to genetic differences generated during the transformation event of the host strain. Furthermore, the cluster analysis showed that the transformants with lesser genetic differences with respect to the P. pastoris host strain are the recombinant strains with the highest level of recombinant protein production. BioMed Central 2006 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/27213/1/217.pdf http://eprints.uanl.mx/27213/1.haspreviewThumbnailVersion/217.pdf Viader Salvadó, José María y Cab Barrera, Eddy Luz y Galán Wong, Luis Jesús y Guerrero Olazarán, Martha (2006) Genotyping of recombinant Pichia pastoris strains. Cellular and Molecular Biology Letters, 11 (3). pp. 348-359. ISSN 1689-1392 http://doi.org/10.2478/s11658-006-0029-z doi:10.2478/s11658-006-0029-z |
| spellingShingle | QR Microbiología Viader Salvadó, José María Cab Barrera, Eddy Luz Galán Wong, Luis Jesús Guerrero Olazarán, Martha Genotyping of recombinant Pichia pastoris strains |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Genotyping of recombinant Pichia pastoris strains |
| title_full | Genotyping of recombinant Pichia pastoris strains |
| title_fullStr | Genotyping of recombinant Pichia pastoris strains |
| title_full_unstemmed | Genotyping of recombinant Pichia pastoris strains |
| title_short | Genotyping of recombinant Pichia pastoris strains |
| title_sort | genotyping of recombinant pichia pastoris strains |
| topic | QR Microbiología |
| url | http://eprints.uanl.mx/27213/1/217.pdf |
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