Genotyping of recombinant Pichia pastoris strains

A simplified amplified-fragment length polymorphism (AFLP) method was used to genotype Pichia pastoris strains obtained by transformation of P. pastoris strain GS115 with a single integration vector. A total of 14 transformants and 3 control strains were analyzed, which generated 16 different band...

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Autores principales: Viader Salvadó, José María, Cab Barrera, Eddy Luz, Galán Wong, Luis Jesús, Guerrero Olazarán, Martha
Formato: Artículo
Lenguaje:inglés
Publicado: BioMed Central 2006
Materias:
Acceso en línea:http://eprints.uanl.mx/27213/1/217.pdf
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author Viader Salvadó, José María
Cab Barrera, Eddy Luz
Galán Wong, Luis Jesús
Guerrero Olazarán, Martha
author_facet Viader Salvadó, José María
Cab Barrera, Eddy Luz
Galán Wong, Luis Jesús
Guerrero Olazarán, Martha
author_sort Viader Salvadó, José María
collection Repositorio Institucional
description A simplified amplified-fragment length polymorphism (AFLP) method was used to genotype Pichia pastoris strains obtained by transformation of P. pastoris strain GS115 with a single integration vector. A total of 14 transformants and 3 control strains were analyzed, which generated 16 different band patterns. A clonal variation was obtained after the transformation process due to genetic differences generated during the transformation event of the host strain. Furthermore, the cluster analysis showed that the transformants with lesser genetic differences with respect to the P. pastoris host strain are the recombinant strains with the highest level of recombinant protein production.
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spelling eprints-272132024-04-23T14:28:58Z http://eprints.uanl.mx/27213/ Genotyping of recombinant Pichia pastoris strains Viader Salvadó, José María Cab Barrera, Eddy Luz Galán Wong, Luis Jesús Guerrero Olazarán, Martha QR Microbiología A simplified amplified-fragment length polymorphism (AFLP) method was used to genotype Pichia pastoris strains obtained by transformation of P. pastoris strain GS115 with a single integration vector. A total of 14 transformants and 3 control strains were analyzed, which generated 16 different band patterns. A clonal variation was obtained after the transformation process due to genetic differences generated during the transformation event of the host strain. Furthermore, the cluster analysis showed that the transformants with lesser genetic differences with respect to the P. pastoris host strain are the recombinant strains with the highest level of recombinant protein production. BioMed Central 2006 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/27213/1/217.pdf http://eprints.uanl.mx/27213/1.haspreviewThumbnailVersion/217.pdf Viader Salvadó, José María y Cab Barrera, Eddy Luz y Galán Wong, Luis Jesús y Guerrero Olazarán, Martha (2006) Genotyping of recombinant Pichia pastoris strains. Cellular and Molecular Biology Letters, 11 (3). pp. 348-359. ISSN 1689-1392 http://doi.org/10.2478/s11658-006-0029-z doi:10.2478/s11658-006-0029-z
spellingShingle QR Microbiología
Viader Salvadó, José María
Cab Barrera, Eddy Luz
Galán Wong, Luis Jesús
Guerrero Olazarán, Martha
Genotyping of recombinant Pichia pastoris strains
thumbnail https://rediab.uanl.mx/themes/sandal5/images/online.png
title Genotyping of recombinant Pichia pastoris strains
title_full Genotyping of recombinant Pichia pastoris strains
title_fullStr Genotyping of recombinant Pichia pastoris strains
title_full_unstemmed Genotyping of recombinant Pichia pastoris strains
title_short Genotyping of recombinant Pichia pastoris strains
title_sort genotyping of recombinant pichia pastoris strains
topic QR Microbiología
url http://eprints.uanl.mx/27213/1/217.pdf
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