Increased Hirsutella citriformis conidia shelf life in Acacia and Hirsutella gum formulations
: Biological control by beneficial microorganisms is known to significantly reduce the effect of pests on crops yield. Among the biocontrol strategies is the use of entomopathogenic fungi such as Hirsutella citriformis, which has been applied to infect and kill hemipteran insect pests, including Dia...
| Autores principales: | , , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
Molecular Diversity Preservation International
2023
|
| Materias: | |
| Acceso en línea: | http://eprints.uanl.mx/26862/1/88.pdf |
| _version_ | 1838551010013872128 |
|---|---|
| author | Flores Villarreal, Rosa Ángela Orozco Flores, Alonso Alberto Cantú Bernal, Servando Horacio Gómez Flores, Ricardo Alberto Pérez González, Orquídea Tamez Guerra, Patricia |
| author_facet | Flores Villarreal, Rosa Ángela Orozco Flores, Alonso Alberto Cantú Bernal, Servando Horacio Gómez Flores, Ricardo Alberto Pérez González, Orquídea Tamez Guerra, Patricia |
| author_sort | Flores Villarreal, Rosa Ángela |
| collection | Repositorio Institucional |
| description | : Biological control by beneficial microorganisms is known to significantly reduce the effect of pests on crops yield. Among the biocontrol strategies is the use of entomopathogenic fungi such as Hirsutella citriformis, which has been applied to infect and kill hemipteran insect pests, including Diaphorina citri Kuwayama (Hemiptera: Liviidae) and Bactericera cockerelli Sulc. (Hemiptera: Triozidae). These biological agents are applied in the form of conidia that are often combined with other inert materials to facilitate application, protect conidia, and improve their shelf life. The aim of this study was to implement strategies for developing formulations to increase conidia shelf life. We evaluated gum produced from one strain and conidia from two different H. citriformis strains. Conidia were formulated by evaluating different concentrations of Acacia and Hirsutella gums to enhance conidia viability during storage at 4 ◦C or 25 ◦C. Results indicated that formulations maintained conidia viability for at least 90 d after storage at 25 ◦C (≥70% viability) and at least 120 d after storage at 4 ◦C, which was significantly (p ≤ 0.05) higher than that of the control, without observing changes in pH values. We also demonstrated 100% formulation purity from days 0 to 120, among all treatments. In conclusion, evaluated formulations maintained H. citriformis conidia viability for at least three months, when stored at 4 ◦C. |
| format | Article |
| id | eprints-26862 |
| institution | UANL |
| language | English |
| publishDate | 2023 |
| publisher | Molecular Diversity Preservation International |
| record_format | eprints |
| spelling | eprints-268622025-07-09T14:37:31Z http://eprints.uanl.mx/26862/ Increased Hirsutella citriformis conidia shelf life in Acacia and Hirsutella gum formulations Flores Villarreal, Rosa Ángela Orozco Flores, Alonso Alberto Cantú Bernal, Servando Horacio Gómez Flores, Ricardo Alberto Pérez González, Orquídea Tamez Guerra, Patricia QR Microbiología : Biological control by beneficial microorganisms is known to significantly reduce the effect of pests on crops yield. Among the biocontrol strategies is the use of entomopathogenic fungi such as Hirsutella citriformis, which has been applied to infect and kill hemipteran insect pests, including Diaphorina citri Kuwayama (Hemiptera: Liviidae) and Bactericera cockerelli Sulc. (Hemiptera: Triozidae). These biological agents are applied in the form of conidia that are often combined with other inert materials to facilitate application, protect conidia, and improve their shelf life. The aim of this study was to implement strategies for developing formulations to increase conidia shelf life. We evaluated gum produced from one strain and conidia from two different H. citriformis strains. Conidia were formulated by evaluating different concentrations of Acacia and Hirsutella gums to enhance conidia viability during storage at 4 ◦C or 25 ◦C. Results indicated that formulations maintained conidia viability for at least 90 d after storage at 25 ◦C (≥70% viability) and at least 120 d after storage at 4 ◦C, which was significantly (p ≤ 0.05) higher than that of the control, without observing changes in pH values. We also demonstrated 100% formulation purity from days 0 to 120, among all treatments. In conclusion, evaluated formulations maintained H. citriformis conidia viability for at least three months, when stored at 4 ◦C. Molecular Diversity Preservation International 2023 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/26862/1/88.pdf http://eprints.uanl.mx/26862/1.haspreviewThumbnailVersion/88.pdf Flores Villarreal, Rosa Ángela y Orozco Flores, Alonso Alberto y Cantú Bernal, Servando Horacio y Gómez Flores, Ricardo Alberto y Pérez González, Orquídea y Tamez Guerra, Patricia (2023) Increased Hirsutella citriformis conidia shelf life in Acacia and Hirsutella gum formulations. Applied Sciences, 13 (13). p. 7912. ISSN 2076-3417 http://doi.org/10.3390/app13137912 doi:10.3390/app13137912 |
| spellingShingle | QR Microbiología Flores Villarreal, Rosa Ángela Orozco Flores, Alonso Alberto Cantú Bernal, Servando Horacio Gómez Flores, Ricardo Alberto Pérez González, Orquídea Tamez Guerra, Patricia Increased Hirsutella citriformis conidia shelf life in Acacia and Hirsutella gum formulations |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Increased Hirsutella citriformis conidia shelf life in Acacia and Hirsutella gum formulations |
| title_full | Increased Hirsutella citriformis conidia shelf life in Acacia and Hirsutella gum formulations |
| title_fullStr | Increased Hirsutella citriformis conidia shelf life in Acacia and Hirsutella gum formulations |
| title_full_unstemmed | Increased Hirsutella citriformis conidia shelf life in Acacia and Hirsutella gum formulations |
| title_short | Increased Hirsutella citriformis conidia shelf life in Acacia and Hirsutella gum formulations |
| title_sort | increased hirsutella citriformis conidia shelf life in acacia and hirsutella gum formulations |
| topic | QR Microbiología |
| url | http://eprints.uanl.mx/26862/1/88.pdf |
| work_keys_str_mv | AT floresvillarrealrosaangela increasedhirsutellacitriformisconidiashelflifeinacaciaandhirsutellagumformulations AT orozcofloresalonsoalberto increasedhirsutellacitriformisconidiashelflifeinacaciaandhirsutellagumformulations AT cantubernalservandohoracio increasedhirsutellacitriformisconidiashelflifeinacaciaandhirsutellagumformulations AT gomezfloresricardoalberto increasedhirsutellacitriformisconidiashelflifeinacaciaandhirsutellagumformulations AT perezgonzalezorquidea increasedhirsutellacitriformisconidiashelflifeinacaciaandhirsutellagumformulations AT tamezguerrapatricia increasedhirsutellacitriformisconidiashelflifeinacaciaandhirsutellagumformulations |