Cumulative antitumor effect of bismuth lipophilic nanoparticles and cetylpyridinium chloride in inhibiting the growth of lung cancer
Objective: To determine the combined antitumor effect of bismuth lipophilic nanoparticles (BisBAL NP) and cetylpyridinium chloride (CPC) on human lung tumor cells. Material and methods: The human lung tumor cells A549 were exposed to 1–100 µM BisBAL NP or CPC, either separately or in a 1:1 combinati...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
Sage Publishing
2023
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| Materias: | |
| Acceso en línea: | http://eprints.uanl.mx/25650/1/25650.pdf |
| _version_ | 1824420268610682880 |
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| author | García Cuellar, Claudia María Hernández Delgadillo, René Torres Betancourt, Jesús Alejandro Solís Soto, Juan Manuel Meester, Irene Sánchez Pérez, Yesennia Pineda Aguilar, Nayely Nakagoshi Cepeda, Sergio Eduardo Sánchez Nájera, Rosa Isela Nakagoshi Cepeda, María Argelia Akemi Chellam, Shankararaman Cabral Romero, Claudio |
| author_facet | García Cuellar, Claudia María Hernández Delgadillo, René Torres Betancourt, Jesús Alejandro Solís Soto, Juan Manuel Meester, Irene Sánchez Pérez, Yesennia Pineda Aguilar, Nayely Nakagoshi Cepeda, Sergio Eduardo Sánchez Nájera, Rosa Isela Nakagoshi Cepeda, María Argelia Akemi Chellam, Shankararaman Cabral Romero, Claudio |
| author_sort | García Cuellar, Claudia María |
| collection | Repositorio Institucional |
| description | Objective: To determine the combined antitumor effect of bismuth lipophilic nanoparticles (BisBAL NP) and cetylpyridinium chloride (CPC) on human lung tumor cells. Material and methods: The human lung tumor cells A549 were exposed to 1–100 µM BisBAL NP or CPC, either separately or in a 1:1 combination. Cell viability was measured with the PrestoBlue assay, the LIVE/DEAD assay, and fluorescence microscopy. The integrity and morphology of cellular microtubules were analyzed by immunofluorescence. Results: A 24-h exposure to 1 µM solutions reduced A549 growth with 21.5% for BisBAL NP, 70.5% for CPC, and 92.4% for the combination ( p < 0.0001), while a 50 µM BisBAL NP/CPC mixture inhibited cell growth with 99% ( p < 0.0001). BisBAL NP-curcumin conjugates were internalized within 30 min of exposure and could be traced within the nucleus of tumor cells within 2 h. BisBAL NP, but not CPC, interfered with microtubule organization, thus interrupting cell replication, similar to the action mechanism of docetaxel. Conclusion: The growth inhibition of A549 human tumor cells by BisBAL NP and CPC was cumulative as of 1 µM. The BisBAL NP/CPC combination may constitute an innovative and cost-effective alternative for treating human lung cancer. |
| format | Article |
| id | eprints-25650 |
| institution | UANL |
| language | English |
| publishDate | 2023 |
| publisher | Sage Publishing |
| record_format | eprints |
| spelling | eprints-256502023-06-28T19:13:00Z http://eprints.uanl.mx/25650/ Cumulative antitumor effect of bismuth lipophilic nanoparticles and cetylpyridinium chloride in inhibiting the growth of lung cancer García Cuellar, Claudia María Hernández Delgadillo, René Torres Betancourt, Jesús Alejandro Solís Soto, Juan Manuel Meester, Irene Sánchez Pérez, Yesennia Pineda Aguilar, Nayely Nakagoshi Cepeda, Sergio Eduardo Sánchez Nájera, Rosa Isela Nakagoshi Cepeda, María Argelia Akemi Chellam, Shankararaman Cabral Romero, Claudio TP Tecnología Química Objective: To determine the combined antitumor effect of bismuth lipophilic nanoparticles (BisBAL NP) and cetylpyridinium chloride (CPC) on human lung tumor cells. Material and methods: The human lung tumor cells A549 were exposed to 1–100 µM BisBAL NP or CPC, either separately or in a 1:1 combination. Cell viability was measured with the PrestoBlue assay, the LIVE/DEAD assay, and fluorescence microscopy. The integrity and morphology of cellular microtubules were analyzed by immunofluorescence. Results: A 24-h exposure to 1 µM solutions reduced A549 growth with 21.5% for BisBAL NP, 70.5% for CPC, and 92.4% for the combination ( p < 0.0001), while a 50 µM BisBAL NP/CPC mixture inhibited cell growth with 99% ( p < 0.0001). BisBAL NP-curcumin conjugates were internalized within 30 min of exposure and could be traced within the nucleus of tumor cells within 2 h. BisBAL NP, but not CPC, interfered with microtubule organization, thus interrupting cell replication, similar to the action mechanism of docetaxel. Conclusion: The growth inhibition of A549 human tumor cells by BisBAL NP and CPC was cumulative as of 1 µM. The BisBAL NP/CPC combination may constitute an innovative and cost-effective alternative for treating human lung cancer. Sage Publishing 2023 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/25650/1/25650.pdf http://eprints.uanl.mx/25650/1.haspreviewThumbnailVersion/25650.pdf García Cuellar, Claudia María y Hernández Delgadillo, René y Torres Betancourt, Jesús Alejandro y Solís Soto, Juan Manuel y Meester, Irene y Sánchez Pérez, Yesennia y Pineda Aguilar, Nayely y Nakagoshi Cepeda, Sergio Eduardo y Sánchez Nájera, Rosa Isela y Nakagoshi Cepeda, María Argelia Akemi y Chellam, Shankararaman y Cabral Romero, Claudio (2023) Cumulative antitumor effect of bismuth lipophilic nanoparticles and cetylpyridinium chloride in inhibiting the growth of lung cancer. Journal of Applied Biomaterials & Functional Materials, 21. pp. 1-10. ISSN 2280-8000 http://doi.org/10.1177/22808000231161177 doi:10.1177/22808000231161177 |
| spellingShingle | TP Tecnología Química García Cuellar, Claudia María Hernández Delgadillo, René Torres Betancourt, Jesús Alejandro Solís Soto, Juan Manuel Meester, Irene Sánchez Pérez, Yesennia Pineda Aguilar, Nayely Nakagoshi Cepeda, Sergio Eduardo Sánchez Nájera, Rosa Isela Nakagoshi Cepeda, María Argelia Akemi Chellam, Shankararaman Cabral Romero, Claudio Cumulative antitumor effect of bismuth lipophilic nanoparticles and cetylpyridinium chloride in inhibiting the growth of lung cancer |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Cumulative antitumor effect of bismuth lipophilic nanoparticles and cetylpyridinium chloride in inhibiting the growth of lung cancer |
| title_full | Cumulative antitumor effect of bismuth lipophilic nanoparticles and cetylpyridinium chloride in inhibiting the growth of lung cancer |
| title_fullStr | Cumulative antitumor effect of bismuth lipophilic nanoparticles and cetylpyridinium chloride in inhibiting the growth of lung cancer |
| title_full_unstemmed | Cumulative antitumor effect of bismuth lipophilic nanoparticles and cetylpyridinium chloride in inhibiting the growth of lung cancer |
| title_short | Cumulative antitumor effect of bismuth lipophilic nanoparticles and cetylpyridinium chloride in inhibiting the growth of lung cancer |
| title_sort | cumulative antitumor effect of bismuth lipophilic nanoparticles and cetylpyridinium chloride in inhibiting the growth of lung cancer |
| topic | TP Tecnología Química |
| url | http://eprints.uanl.mx/25650/1/25650.pdf |
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