In Vivo Antitumor Activity of Metal Silver and Silver Nanoparticles in the L5178Y-R Murine Lymphoma Model
Aims: To evaluate the antitumor potential of metal silver and polyvinilpyrrolidone nanoparticle-encapsulated silver on L5178Y-R murine lymphoma cell growth and survival of tumor-bearing mice. Study Design: In vitro and in vivo (pre-clinical) study. Place and Duration of Study: Universidad Autónoma...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Spanish / Castilian |
| Publicado: |
Sciencedomain International
2013
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| Materias: | |
| Acceso en línea: | http://eprints.uanl.mx/25039/1/25039.pdf |
| _version_ | 1838551007368314880 |
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| author | Lara González, José Horacio Gómez Flores, Ricardo Alberto Tamez Guerra, Patricia Monreal Cuevas, Enriqueta Tamez Guerra, Reyes Silvestre Rodríguez Padilla, Cristina |
| author_facet | Lara González, José Horacio Gómez Flores, Ricardo Alberto Tamez Guerra, Patricia Monreal Cuevas, Enriqueta Tamez Guerra, Reyes Silvestre Rodríguez Padilla, Cristina |
| author_sort | Lara González, José Horacio |
| collection | Repositorio Institucional |
| description | Aims: To evaluate the antitumor potential of metal silver and polyvinilpyrrolidone nanoparticle-encapsulated silver on L5178Y-R murine lymphoma cell growth and survival of tumor-bearing mice.
Study Design: In vitro and in vivo (pre-clinical) study.
Place and Duration of Study: Universidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Departamento de Microbiología e Inmunología, San Nicolás de los Garza, N.L., México, from January 2009 to December 2011.
Methodology: Concentration-response cell viability assay was performed in vitro and mice survival studies were done using a L5178Y-R tumor-bearing mouse model. The PROBIT regression analysis was performed to determine the in vitro LC50. In vivo survival distributions were calculated by Kaplan-Meier and Cutler-Ederer analysis, and survival curves comparisons and hypothesis testing was done using the log-rank
method.
Results: Metal silver induced up to 100% L5178Y-R cells cytotoxicity, with an LC50 of 1.8 X 10-8 M, whereas silver nanoparticles caused up to 78% cytotoxicity, with an LC50 of
14.4 X 10-8 M. In addition, Intramuscular administration of metal silver and silver nanoparticles administered at the time of tumor injection significantly (P = .05) increased
mice survival, where 70% and 60% of mice survived at day 35 respectively, as compared with such treatments administered 7 days after tumor induction (55% and 25% survival respectively); vincristine treatment caused 50% mice survival and tumor-bearing control mice had 20% survival. These results open further approaches on treating several types of cancer using free and nanoparticle-encapsulated silver-based therapies. |
| format | Article |
| id | eprints-25039 |
| institution | UANL |
| language | Spanish / Castilian |
| publishDate | 2013 |
| publisher | Sciencedomain International |
| record_format | eprints |
| spelling | eprints-250392025-07-10T16:56:56Z http://eprints.uanl.mx/25039/ In Vivo Antitumor Activity of Metal Silver and Silver Nanoparticles in the L5178Y-R Murine Lymphoma Model Lara González, José Horacio Gómez Flores, Ricardo Alberto Tamez Guerra, Patricia Monreal Cuevas, Enriqueta Tamez Guerra, Reyes Silvestre Rodríguez Padilla, Cristina QH Historia Natural, Biología Aims: To evaluate the antitumor potential of metal silver and polyvinilpyrrolidone nanoparticle-encapsulated silver on L5178Y-R murine lymphoma cell growth and survival of tumor-bearing mice. Study Design: In vitro and in vivo (pre-clinical) study. Place and Duration of Study: Universidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Departamento de Microbiología e Inmunología, San Nicolás de los Garza, N.L., México, from January 2009 to December 2011. Methodology: Concentration-response cell viability assay was performed in vitro and mice survival studies were done using a L5178Y-R tumor-bearing mouse model. The PROBIT regression analysis was performed to determine the in vitro LC50. In vivo survival distributions were calculated by Kaplan-Meier and Cutler-Ederer analysis, and survival curves comparisons and hypothesis testing was done using the log-rank method. Results: Metal silver induced up to 100% L5178Y-R cells cytotoxicity, with an LC50 of 1.8 X 10-8 M, whereas silver nanoparticles caused up to 78% cytotoxicity, with an LC50 of 14.4 X 10-8 M. In addition, Intramuscular administration of metal silver and silver nanoparticles administered at the time of tumor injection significantly (P = .05) increased mice survival, where 70% and 60% of mice survived at day 35 respectively, as compared with such treatments administered 7 days after tumor induction (55% and 25% survival respectively); vincristine treatment caused 50% mice survival and tumor-bearing control mice had 20% survival. These results open further approaches on treating several types of cancer using free and nanoparticle-encapsulated silver-based therapies. Sciencedomain International 2013 Article PeerReviewed text es cc_by_nc_nd http://eprints.uanl.mx/25039/1/25039.pdf http://eprints.uanl.mx/25039/1.haspreviewThumbnailVersion/25039.pdf Lara González, José Horacio y Gómez Flores, Ricardo Alberto y Tamez Guerra, Patricia y Monreal Cuevas, Enriqueta y Tamez Guerra, Reyes Silvestre y Rodríguez Padilla, Cristina (2013) In Vivo Antitumor Activity of Metal Silver and Silver Nanoparticles in the L5178Y-R Murine Lymphoma Model. British Journal of Medicine & Medical Research, 3 (4). pp. 1308-1316. ISSN 2231-0614 |
| spellingShingle | QH Historia Natural, Biología Lara González, José Horacio Gómez Flores, Ricardo Alberto Tamez Guerra, Patricia Monreal Cuevas, Enriqueta Tamez Guerra, Reyes Silvestre Rodríguez Padilla, Cristina In Vivo Antitumor Activity of Metal Silver and Silver Nanoparticles in the L5178Y-R Murine Lymphoma Model |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | In Vivo Antitumor Activity of Metal Silver and Silver Nanoparticles in the L5178Y-R Murine Lymphoma Model |
| title_full | In Vivo Antitumor Activity of Metal Silver and Silver Nanoparticles in the L5178Y-R Murine Lymphoma Model |
| title_fullStr | In Vivo Antitumor Activity of Metal Silver and Silver Nanoparticles in the L5178Y-R Murine Lymphoma Model |
| title_full_unstemmed | In Vivo Antitumor Activity of Metal Silver and Silver Nanoparticles in the L5178Y-R Murine Lymphoma Model |
| title_short | In Vivo Antitumor Activity of Metal Silver and Silver Nanoparticles in the L5178Y-R Murine Lymphoma Model |
| title_sort | in vivo antitumor activity of metal silver and silver nanoparticles in the l5178y r murine lymphoma model |
| topic | QH Historia Natural, Biología |
| url | http://eprints.uanl.mx/25039/1/25039.pdf |
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