Assessment of nutrient allocation and metabolic turnover rate in Pacific white shrimp Litopenaeus vannamei co-fed live macroalgae Ulva clathrata and inert feed: dual stable isotope analysis

The present study quantified the relative contribution of dietary carbon and nitrogen supplied by live biomass of the green macroalgae Ulva clathrata and a commercial inert feed to the growth of juvenile shrimp Litopenaeus vannamei. The stable isotope ratios of carbon and nitrogen (δ13C and δ15N) we...

Full description

Bibliographic Details
Main Authors: Gamboa Delgado, Julián, Peña Rodríguez, Alberto, Ricque Marie, Denis, Cruz Suárez, Lucía Elizabeth
Format: Article
Language:English
Published: National Shellfisheries Association 2011
Subjects:
Online Access:http://eprints.uanl.mx/2409/1/Shrimp-Ulva_%28JSR%29.pdf
_version_ 1824368250679459840
author Gamboa Delgado, Julián
Peña Rodríguez, Alberto
Ricque Marie, Denis
Cruz Suárez, Lucía Elizabeth
author_facet Gamboa Delgado, Julián
Peña Rodríguez, Alberto
Ricque Marie, Denis
Cruz Suárez, Lucía Elizabeth
author_sort Gamboa Delgado, Julián
collection Repositorio Institucional
description The present study quantified the relative contribution of dietary carbon and nitrogen supplied by live biomass of the green macroalgae Ulva clathrata and a commercial inert feed to the growth of juvenile shrimp Litopenaeus vannamei. The stable isotope ratios of carbon and nitrogen (δ13C and δ15N) were analyzed in both food sources, whole bodies and muscle tissue of shrimp reared on co-feeding regimes where 75, 50 and 25% of daily consumed macroalgal biomass was substituted by inert feed (regimes 75F/25U, 50F/50U and 25F/75U, respectively). Higher growth rates were observed in shrimps fed on regime 75F/25U (k = 0.062), followed by shrimps fed only inert feed (100F, k = 0.060). Animals reared only on U. clathrata (100U) showed minimal growth (k = 0.008) and very high metabolic turnover rates of carbon and nitrogen. Isotopic values measured in inert feed (δ13C= -23.0 ‰, δ15N= 9.7‰) and macroalgae (δ13C= -13.1‰, δ15N= -3.5‰) were very contrasting and both had a rapid influence on the isotopic values of shrimp. Animals reached full isotopic equilibrium through growth and fast metabolic turnover in only 2 weeks, except shrimps fed only macroalgae. At the end of the experiment, δ13C and δ15N values in shrimp reared on all co-feeding regimes were strongly biased towards the isotopic values of U. clathrata. Total dry matter contributions to growth were estimated using an isotope mixing model and considering the elemental concentration of both nutritional sources. Results indicated that shrimps in the co-feeding regimes incorporated significantly higher amounts of dietary carbon and nitrogen from the macroalgal biomass. Shrimps in treatment 75F/25U incorporated 52% of carbon from the inert feed and 48% from the macroalgae. Animals under feeding regimes 50F/50U and 25F/75U incorporated higher amounts of dietary carbon from U. clathrata (65-89%) when compared to carbon proportions supplied by both co-feeding regimes (33-70%) and also incorporated the majority of nitrogen from the macroalgae. However, a high incorporation of nitrogen was not reflected in high growth in the latter treatments as metabolic turnover rates were very high. Estimated turnover rates ranged from 0.049 to 0.191 d-1 for carbon and from 0.013 to 0.100 d-1 for nitrogen and values followed an increasing trend as a function of macroalgae consumption. Nitrogen half-times in tissue consistently decreased throughout the different treatments from 9.5 d (100F) to 6.4 d (100U). Proportions of incorporated nutrients in muscle tissue followed similar patterns as those observed in whole bodies.
format Article
id eprints-2409
institution UANL
language English
publishDate 2011
publisher National Shellfisheries Association
record_format eprints
spelling eprints-24092014-11-24T20:30:21Z http://eprints.uanl.mx/2409/ Assessment of nutrient allocation and metabolic turnover rate in Pacific white shrimp Litopenaeus vannamei co-fed live macroalgae Ulva clathrata and inert feed: dual stable isotope analysis Gamboa Delgado, Julián Peña Rodríguez, Alberto Ricque Marie, Denis Cruz Suárez, Lucía Elizabeth SH Acuacultura / Pesca The present study quantified the relative contribution of dietary carbon and nitrogen supplied by live biomass of the green macroalgae Ulva clathrata and a commercial inert feed to the growth of juvenile shrimp Litopenaeus vannamei. The stable isotope ratios of carbon and nitrogen (δ13C and δ15N) were analyzed in both food sources, whole bodies and muscle tissue of shrimp reared on co-feeding regimes where 75, 50 and 25% of daily consumed macroalgal biomass was substituted by inert feed (regimes 75F/25U, 50F/50U and 25F/75U, respectively). Higher growth rates were observed in shrimps fed on regime 75F/25U (k = 0.062), followed by shrimps fed only inert feed (100F, k = 0.060). Animals reared only on U. clathrata (100U) showed minimal growth (k = 0.008) and very high metabolic turnover rates of carbon and nitrogen. Isotopic values measured in inert feed (δ13C= -23.0 ‰, δ15N= 9.7‰) and macroalgae (δ13C= -13.1‰, δ15N= -3.5‰) were very contrasting and both had a rapid influence on the isotopic values of shrimp. Animals reached full isotopic equilibrium through growth and fast metabolic turnover in only 2 weeks, except shrimps fed only macroalgae. At the end of the experiment, δ13C and δ15N values in shrimp reared on all co-feeding regimes were strongly biased towards the isotopic values of U. clathrata. Total dry matter contributions to growth were estimated using an isotope mixing model and considering the elemental concentration of both nutritional sources. Results indicated that shrimps in the co-feeding regimes incorporated significantly higher amounts of dietary carbon and nitrogen from the macroalgal biomass. Shrimps in treatment 75F/25U incorporated 52% of carbon from the inert feed and 48% from the macroalgae. Animals under feeding regimes 50F/50U and 25F/75U incorporated higher amounts of dietary carbon from U. clathrata (65-89%) when compared to carbon proportions supplied by both co-feeding regimes (33-70%) and also incorporated the majority of nitrogen from the macroalgae. However, a high incorporation of nitrogen was not reflected in high growth in the latter treatments as metabolic turnover rates were very high. Estimated turnover rates ranged from 0.049 to 0.191 d-1 for carbon and from 0.013 to 0.100 d-1 for nitrogen and values followed an increasing trend as a function of macroalgae consumption. Nitrogen half-times in tissue consistently decreased throughout the different treatments from 9.5 d (100F) to 6.4 d (100U). Proportions of incorporated nutrients in muscle tissue followed similar patterns as those observed in whole bodies. National Shellfisheries Association 2011-12-07 Article PeerReviewed application/pdf en http://eprints.uanl.mx/2409/1/Shrimp-Ulva_%28JSR%29.pdf http://eprints.uanl.mx/2409/1.haspreviewThumbnailVersion/Shrimp-Ulva_%28JSR%29.pdf Gamboa Delgado, Julián y Peña Rodríguez, Alberto y Ricque Marie, Denis y Cruz Suárez, Lucía Elizabeth (2011) Assessment of nutrient allocation and metabolic turnover rate in Pacific white shrimp Litopenaeus vannamei co-fed live macroalgae Ulva clathrata and inert feed: dual stable isotope analysis. Journal of Shellfish Research, 30 (3). pp. 969-978. ISSN 0730-8000 http://www.bioone.org/toc/shre/current
spellingShingle SH Acuacultura / Pesca
Gamboa Delgado, Julián
Peña Rodríguez, Alberto
Ricque Marie, Denis
Cruz Suárez, Lucía Elizabeth
Assessment of nutrient allocation and metabolic turnover rate in Pacific white shrimp Litopenaeus vannamei co-fed live macroalgae Ulva clathrata and inert feed: dual stable isotope analysis
thumbnail https://rediab.uanl.mx/themes/sandal5/images/online.png
title Assessment of nutrient allocation and metabolic turnover rate in Pacific white shrimp Litopenaeus vannamei co-fed live macroalgae Ulva clathrata and inert feed: dual stable isotope analysis
title_full Assessment of nutrient allocation and metabolic turnover rate in Pacific white shrimp Litopenaeus vannamei co-fed live macroalgae Ulva clathrata and inert feed: dual stable isotope analysis
title_fullStr Assessment of nutrient allocation and metabolic turnover rate in Pacific white shrimp Litopenaeus vannamei co-fed live macroalgae Ulva clathrata and inert feed: dual stable isotope analysis
title_full_unstemmed Assessment of nutrient allocation and metabolic turnover rate in Pacific white shrimp Litopenaeus vannamei co-fed live macroalgae Ulva clathrata and inert feed: dual stable isotope analysis
title_short Assessment of nutrient allocation and metabolic turnover rate in Pacific white shrimp Litopenaeus vannamei co-fed live macroalgae Ulva clathrata and inert feed: dual stable isotope analysis
title_sort assessment of nutrient allocation and metabolic turnover rate in pacific white shrimp litopenaeus vannamei co fed live macroalgae ulva clathrata and inert feed dual stable isotope analysis
topic SH Acuacultura / Pesca
url http://eprints.uanl.mx/2409/1/Shrimp-Ulva_%28JSR%29.pdf
work_keys_str_mv AT gamboadelgadojulian assessmentofnutrientallocationandmetabolicturnoverrateinpacificwhiteshrimplitopenaeusvannameicofedlivemacroalgaeulvaclathrataandinertfeeddualstableisotopeanalysis
AT penarodriguezalberto assessmentofnutrientallocationandmetabolicturnoverrateinpacificwhiteshrimplitopenaeusvannameicofedlivemacroalgaeulvaclathrataandinertfeeddualstableisotopeanalysis
AT ricquemariedenis assessmentofnutrientallocationandmetabolicturnoverrateinpacificwhiteshrimplitopenaeusvannameicofedlivemacroalgaeulvaclathrataandinertfeeddualstableisotopeanalysis
AT cruzsuarezluciaelizabeth assessmentofnutrientallocationandmetabolicturnoverrateinpacificwhiteshrimplitopenaeusvannameicofedlivemacroalgaeulvaclathrataandinertfeeddualstableisotopeanalysis