Effect of different diets on proteolytic enzyme activity, trypsinogen gene expression and dietary carbon assimilation in Senegalese sole (Solea senegalensis) larvae
The effect of diet on larval growth, anionic trypsinogen gene expression (ssetryp1), and trypsin and chymotrypsin activities was assessed in Solea senegalensis. Changes in larval carbon stable isotope (δ13C) composition were used to estimate carbon assimilation. Diets were supplied for 20 d to fish...
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| Format: | Article |
| Language: | English |
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2011
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| Online Access: | http://eprints.uanl.mx/2407/1/CBP_MS18517.pdf |
| _version_ | 1824368249954893824 |
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| author | Gamboa Delgado, Julián Le Vay, Lewis Fernández Díaz, Catalina Cañavate, José Pedro Ponce, Marian Zerolo, Ricardo Manchado, Manuel |
| author_facet | Gamboa Delgado, Julián Le Vay, Lewis Fernández Díaz, Catalina Cañavate, José Pedro Ponce, Marian Zerolo, Ricardo Manchado, Manuel |
| author_sort | Gamboa Delgado, Julián |
| collection | Repositorio Institucional |
| description | The effect of diet on larval growth, anionic trypsinogen gene expression (ssetryp1), and trypsin and chymotrypsin activities was assessed in Solea senegalensis. Changes in larval carbon stable isotope (δ13C) composition were used to estimate carbon assimilation. Diets were supplied for 20 d to fish held in larval rearing tanks and consisted of live rotifers, Artemia nauplii, rotifers followed by Artemia, rotifers co-fed with inert diet and inert diet alone. Growth was significantly faster in larvae fed only Artemia and those fed rotifers and Artemia (k= 0.381-0.387 d-1). Trypsin and chymotrypsin activities increased from 3-4 days after hatching (DAH) in all dietary treatments, while ssetryp1 transcripts increased at 4-5 DAH only in larvae fed live prey. ssetryp1 gene expression was activated later in larvae fed only Artemia and this corresponded with Artemia δ13C values being reflected in larval tissue. Larval δ13C values also indicated greater selection and/or assimilation of rotifers in relation to the inert diet. Results demonstrate that during early larval development of sole, diet modulates ssetryp1 gene expression. The rapid and intense response to diets that promoted different growth and survival suggests the suitability of this biomarker as a nutritional status indicator in early sole larvae. |
| format | Article |
| id | eprints-2407 |
| institution | UANL |
| language | English |
| publishDate | 2011 |
| record_format | eprints |
| spelling | eprints-24072022-04-25T20:44:40Z http://eprints.uanl.mx/2407/ Effect of different diets on proteolytic enzyme activity, trypsinogen gene expression and dietary carbon assimilation in Senegalese sole (Solea senegalensis) larvae Gamboa Delgado, Julián Le Vay, Lewis Fernández Díaz, Catalina Cañavate, José Pedro Ponce, Marian Zerolo, Ricardo Manchado, Manuel SH Acuacultura / Pesca The effect of diet on larval growth, anionic trypsinogen gene expression (ssetryp1), and trypsin and chymotrypsin activities was assessed in Solea senegalensis. Changes in larval carbon stable isotope (δ13C) composition were used to estimate carbon assimilation. Diets were supplied for 20 d to fish held in larval rearing tanks and consisted of live rotifers, Artemia nauplii, rotifers followed by Artemia, rotifers co-fed with inert diet and inert diet alone. Growth was significantly faster in larvae fed only Artemia and those fed rotifers and Artemia (k= 0.381-0.387 d-1). Trypsin and chymotrypsin activities increased from 3-4 days after hatching (DAH) in all dietary treatments, while ssetryp1 transcripts increased at 4-5 DAH only in larvae fed live prey. ssetryp1 gene expression was activated later in larvae fed only Artemia and this corresponded with Artemia δ13C values being reflected in larval tissue. Larval δ13C values also indicated greater selection and/or assimilation of rotifers in relation to the inert diet. Results demonstrate that during early larval development of sole, diet modulates ssetryp1 gene expression. The rapid and intense response to diets that promoted different growth and survival suggests the suitability of this biomarker as a nutritional status indicator in early sole larvae. 2011 Article PeerReviewed application/pdf en http://eprints.uanl.mx/2407/1/CBP_MS18517.pdf http://eprints.uanl.mx/2407/1.haspreviewThumbnailVersion/CBP_MS18517.pdf Gamboa Delgado, Julián y Le Vay, Lewis y Fernández Díaz, Catalina y Cañavate, José Pedro y Ponce, Marian y Zerolo, Ricardo y Manchado, Manuel (2011) Effect of different diets on proteolytic enzyme activity, trypsinogen gene expression and dietary carbon assimilation in Senegalese sole (Solea senegalensis) larvae. Comparative biochemistry and physiology Part B: biochemistry and molecular biology, 158 (4). pp. 251-258. ISSN 1096-4959 http://dx.doi.org/10.1016/j.cbpb.2010.11.012 doi:10.1016/j.cbpb.2010.11.012 |
| spellingShingle | SH Acuacultura / Pesca Gamboa Delgado, Julián Le Vay, Lewis Fernández Díaz, Catalina Cañavate, José Pedro Ponce, Marian Zerolo, Ricardo Manchado, Manuel Effect of different diets on proteolytic enzyme activity, trypsinogen gene expression and dietary carbon assimilation in Senegalese sole (Solea senegalensis) larvae |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Effect of different diets on proteolytic enzyme activity, trypsinogen gene expression and dietary carbon assimilation in Senegalese sole (Solea senegalensis) larvae |
| title_full | Effect of different diets on proteolytic enzyme activity, trypsinogen gene expression and dietary carbon assimilation in Senegalese sole (Solea senegalensis) larvae |
| title_fullStr | Effect of different diets on proteolytic enzyme activity, trypsinogen gene expression and dietary carbon assimilation in Senegalese sole (Solea senegalensis) larvae |
| title_full_unstemmed | Effect of different diets on proteolytic enzyme activity, trypsinogen gene expression and dietary carbon assimilation in Senegalese sole (Solea senegalensis) larvae |
| title_short | Effect of different diets on proteolytic enzyme activity, trypsinogen gene expression and dietary carbon assimilation in Senegalese sole (Solea senegalensis) larvae |
| title_sort | effect of different diets on proteolytic enzyme activity trypsinogen gene expression and dietary carbon assimilation in senegalese sole solea senegalensis larvae |
| topic | SH Acuacultura / Pesca |
| url | http://eprints.uanl.mx/2407/1/CBP_MS18517.pdf |
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