Application of Extractive Fermentation on the Recuperation of Exopolysaccharide from Rhodotorula mucilaginosa UANL-001L

Exopolysaccharides (EPS) are high molecular weight biomaterials of industrial interest due to their variety of applications in the pharmaceutical, cosmetic, environmental, and food industries. EPS produced by Rhodotorula mucilaginosa UANL-001 L has sparked interest due to its bio-adsorbent and wide...

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Main Authors: Medina Ramírez, Carlo Franco, Castañeda Guel, Mariana Teresa, Alvarez González, Ma. Fernanda, Montesinos Castellanos, Alejandro, Morones Ramírez, José Rubén, López Guajardo, Enrique A., Gomez Loredo, Alma Elizabeth
Format: Article
Language:English
Published: Molecular Diversity Preservation International 2020
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Online Access:http://eprints.uanl.mx/23956/1/23956.pdf
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author Medina Ramírez, Carlo Franco
Castañeda Guel, Mariana Teresa
Alvarez González, Ma. Fernanda
Montesinos Castellanos, Alejandro
Morones Ramírez, José Rubén
López Guajardo, Enrique A.
Gomez Loredo, Alma Elizabeth
author_facet Medina Ramírez, Carlo Franco
Castañeda Guel, Mariana Teresa
Alvarez González, Ma. Fernanda
Montesinos Castellanos, Alejandro
Morones Ramírez, José Rubén
López Guajardo, Enrique A.
Gomez Loredo, Alma Elizabeth
author_sort Medina Ramírez, Carlo Franco
collection Repositorio Institucional
description Exopolysaccharides (EPS) are high molecular weight biomaterials of industrial interest due to their variety of applications in the pharmaceutical, cosmetic, environmental, and food industries. EPS produced by Rhodotorula mucilaginosa UANL-001 L has sparked interest due to its bio-adsorbent and wide spectrum antimicrobial properties. However, full exploitation and commercial application of EPS has been restrained due to low yields and high production costs. In the present work, the production and separation of EPS from Rhodotorula mucilaginosa UANL-001L was attempted through extractive fermentation in order to increase EPS production while simplifying the recovery process. Extractive fermentation was implemented with a thermoseparating polymer for phase formation (EOPO 970 and EOPO 12,000); culture viability, biomass generation, EPS production, rheological system properties, and phase formation time and temperature were monitored throughout the process. Extractive fermentation of Rhodotorula mucilaginosa UANL-001L with EOPO 970 resulted in a 42% EPS and 7% biomass recovery on the top phase after 5 to 13-min phase formation time and temperatures between 30 and 40 °C. This is the first report of extractive fermentation application for EPS production by yeast of the genera Rhodotorula, resulting in an interesting strategy for EPS production and recovery, although further optimization is needed.
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spelling eprints-239562024-03-05T20:06:51Z http://eprints.uanl.mx/23956/ Application of Extractive Fermentation on the Recuperation of Exopolysaccharide from Rhodotorula mucilaginosa UANL-001L Medina Ramírez, Carlo Franco Castañeda Guel, Mariana Teresa Alvarez González, Ma. Fernanda Montesinos Castellanos, Alejandro Morones Ramírez, José Rubén López Guajardo, Enrique A. Gomez Loredo, Alma Elizabeth TP Tecnología Química Exopolysaccharides (EPS) are high molecular weight biomaterials of industrial interest due to their variety of applications in the pharmaceutical, cosmetic, environmental, and food industries. EPS produced by Rhodotorula mucilaginosa UANL-001 L has sparked interest due to its bio-adsorbent and wide spectrum antimicrobial properties. However, full exploitation and commercial application of EPS has been restrained due to low yields and high production costs. In the present work, the production and separation of EPS from Rhodotorula mucilaginosa UANL-001L was attempted through extractive fermentation in order to increase EPS production while simplifying the recovery process. Extractive fermentation was implemented with a thermoseparating polymer for phase formation (EOPO 970 and EOPO 12,000); culture viability, biomass generation, EPS production, rheological system properties, and phase formation time and temperature were monitored throughout the process. Extractive fermentation of Rhodotorula mucilaginosa UANL-001L with EOPO 970 resulted in a 42% EPS and 7% biomass recovery on the top phase after 5 to 13-min phase formation time and temperatures between 30 and 40 °C. This is the first report of extractive fermentation application for EPS production by yeast of the genera Rhodotorula, resulting in an interesting strategy for EPS production and recovery, although further optimization is needed. Molecular Diversity Preservation International 2020 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/23956/1/23956.pdf http://eprints.uanl.mx/23956/1.haspreviewThumbnailVersion/23956.pdf Medina Ramírez, Carlo Franco y Castañeda Guel, Mariana Teresa y Alvarez González, Ma. Fernanda y Montesinos Castellanos, Alejandro y Morones Ramírez, José Rubén y López Guajardo, Enrique A. y Gomez Loredo, Alma Elizabeth (2020) Application of Extractive Fermentation on the Recuperation of Exopolysaccharide from Rhodotorula mucilaginosa UANL-001L. Fermentation, 6. pp. 1-12. ISSN 2311-5637 http://doi.org/10.3390/fermentation6040108 doi:10.3390/fermentation6040108
spellingShingle TP Tecnología Química
Medina Ramírez, Carlo Franco
Castañeda Guel, Mariana Teresa
Alvarez González, Ma. Fernanda
Montesinos Castellanos, Alejandro
Morones Ramírez, José Rubén
López Guajardo, Enrique A.
Gomez Loredo, Alma Elizabeth
Application of Extractive Fermentation on the Recuperation of Exopolysaccharide from Rhodotorula mucilaginosa UANL-001L
thumbnail https://rediab.uanl.mx/themes/sandal5/images/online.png
title Application of Extractive Fermentation on the Recuperation of Exopolysaccharide from Rhodotorula mucilaginosa UANL-001L
title_full Application of Extractive Fermentation on the Recuperation of Exopolysaccharide from Rhodotorula mucilaginosa UANL-001L
title_fullStr Application of Extractive Fermentation on the Recuperation of Exopolysaccharide from Rhodotorula mucilaginosa UANL-001L
title_full_unstemmed Application of Extractive Fermentation on the Recuperation of Exopolysaccharide from Rhodotorula mucilaginosa UANL-001L
title_short Application of Extractive Fermentation on the Recuperation of Exopolysaccharide from Rhodotorula mucilaginosa UANL-001L
title_sort application of extractive fermentation on the recuperation of exopolysaccharide from rhodotorula mucilaginosa uanl 001l
topic TP Tecnología Química
url http://eprints.uanl.mx/23956/1/23956.pdf
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