Two New Dihydrosphingosine Analogs Against Mycobacterium tuberculosis Affect gltA1, lprQ, and rpsO Expression
The emergence of multidrug-resistant (MDR) Mycobacterium tuberculosis strains threaten the control of tuberculosis. New antitubercular dihydrosphingosine analogs, named UCIs, have been evaluated in preclinical studies but their cellular and molecular mechanisms of action against M. tuberculosis are...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Research Foundation
2021
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| Online Access: | http://eprints.uanl.mx/23508/1/23508.pdf |
| _version_ | 1824416910150729728 |
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| author | Peñuelas Urquides, Katia Bermúdez de León, Mario Silva Ramírez, Beatriz Castorena Torres, Fabiola Molina Salinas, Gloria María Castro Garza, Jorge Enrique Becerril Montes, Pola Del Olmo, Esther San Feliciano, Arturo González Escalante, Laura Adiene Villarreal Treviño, Licet Said Fernández, Salvador |
| author_facet | Peñuelas Urquides, Katia Bermúdez de León, Mario Silva Ramírez, Beatriz Castorena Torres, Fabiola Molina Salinas, Gloria María Castro Garza, Jorge Enrique Becerril Montes, Pola Del Olmo, Esther San Feliciano, Arturo González Escalante, Laura Adiene Villarreal Treviño, Licet Said Fernández, Salvador |
| author_sort | Peñuelas Urquides, Katia |
| collection | Repositorio Institucional |
| description | The emergence of multidrug-resistant (MDR) Mycobacterium tuberculosis strains threaten the control of tuberculosis. New antitubercular dihydrosphingosine analogs, named UCIs, have been evaluated in preclinical studies but their cellular and molecular mechanisms of action against M. tuberculosis are still unknown. The aim of this study was to evaluate the effect of UCI exposure on gene expression of drug-sensitive H37Rv and MDR CIBIN:UMF:15:99 clones of M. tuberculosis which were isolated, phenotypically, and genetically characterized, cultured to log phase and treated with UCI compounds; followed by total RNA isolation, reverse transcription and hybridization assays on Affymetrix genomic microarrays. Data were validated with RT-qPCR assays. As results, UCI-05 and UCI-14 exposure increased gltA1 expression in drug-sensitive H37Rv clones. Furthermore, UCI-05 increased lprQ expression in MDR CIBIN:UMF:15:99 M. tuberculosis clones while UCI-14 reduced the expression of this gene in drug-sensitive H37Rv clones. In addition, UCI-05 reduced rpsO expression in drug-sensitive H37Rv clones. We found gene expression alterations that suggest these molecules may alter carbon and lipid metabolism as well as interfere in the protein-producing machinery in M. tuberculosis. |
| format | Article |
| id | eprints-23508 |
| institution | UANL |
| language | English |
| publishDate | 2021 |
| publisher | Frontiers Research Foundation |
| record_format | eprints |
| spelling | eprints-235082022-06-30T20:35:36Z http://eprints.uanl.mx/23508/ Two New Dihydrosphingosine Analogs Against Mycobacterium tuberculosis Affect gltA1, lprQ, and rpsO Expression Peñuelas Urquides, Katia Bermúdez de León, Mario Silva Ramírez, Beatriz Castorena Torres, Fabiola Molina Salinas, Gloria María Castro Garza, Jorge Enrique Becerril Montes, Pola Del Olmo, Esther San Feliciano, Arturo González Escalante, Laura Adiene Villarreal Treviño, Licet Said Fernández, Salvador QR Microbiología The emergence of multidrug-resistant (MDR) Mycobacterium tuberculosis strains threaten the control of tuberculosis. New antitubercular dihydrosphingosine analogs, named UCIs, have been evaluated in preclinical studies but their cellular and molecular mechanisms of action against M. tuberculosis are still unknown. The aim of this study was to evaluate the effect of UCI exposure on gene expression of drug-sensitive H37Rv and MDR CIBIN:UMF:15:99 clones of M. tuberculosis which were isolated, phenotypically, and genetically characterized, cultured to log phase and treated with UCI compounds; followed by total RNA isolation, reverse transcription and hybridization assays on Affymetrix genomic microarrays. Data were validated with RT-qPCR assays. As results, UCI-05 and UCI-14 exposure increased gltA1 expression in drug-sensitive H37Rv clones. Furthermore, UCI-05 increased lprQ expression in MDR CIBIN:UMF:15:99 M. tuberculosis clones while UCI-14 reduced the expression of this gene in drug-sensitive H37Rv clones. In addition, UCI-05 reduced rpsO expression in drug-sensitive H37Rv clones. We found gene expression alterations that suggest these molecules may alter carbon and lipid metabolism as well as interfere in the protein-producing machinery in M. tuberculosis. Frontiers Research Foundation 2021 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/23508/1/23508.pdf http://eprints.uanl.mx/23508/1.haspreviewThumbnailVersion/23508.pdf Peñuelas Urquides, Katia y Bermúdez de León, Mario y Silva Ramírez, Beatriz y Castorena Torres, Fabiola y Molina Salinas, Gloria María y Castro Garza, Jorge Enrique y Becerril Montes, Pola y Del Olmo, Esther y San Feliciano, Arturo y González Escalante, Laura Adiene y Villarreal Treviño, Licet y Said Fernández, Salvador (2021) Two New Dihydrosphingosine Analogs Against Mycobacterium tuberculosis Affect gltA1, lprQ, and rpsO Expression. Frontiers in Microbiology, 12. pp. 1-11. ISSN 1664-302X http://doi.org/10.3389/fmicb.2021.742867 doi:10.3389/fmicb.2021.742867 |
| spellingShingle | QR Microbiología Peñuelas Urquides, Katia Bermúdez de León, Mario Silva Ramírez, Beatriz Castorena Torres, Fabiola Molina Salinas, Gloria María Castro Garza, Jorge Enrique Becerril Montes, Pola Del Olmo, Esther San Feliciano, Arturo González Escalante, Laura Adiene Villarreal Treviño, Licet Said Fernández, Salvador Two New Dihydrosphingosine Analogs Against Mycobacterium tuberculosis Affect gltA1, lprQ, and rpsO Expression |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Two New Dihydrosphingosine Analogs Against Mycobacterium tuberculosis Affect gltA1, lprQ, and rpsO Expression |
| title_full | Two New Dihydrosphingosine Analogs Against Mycobacterium tuberculosis Affect gltA1, lprQ, and rpsO Expression |
| title_fullStr | Two New Dihydrosphingosine Analogs Against Mycobacterium tuberculosis Affect gltA1, lprQ, and rpsO Expression |
| title_full_unstemmed | Two New Dihydrosphingosine Analogs Against Mycobacterium tuberculosis Affect gltA1, lprQ, and rpsO Expression |
| title_short | Two New Dihydrosphingosine Analogs Against Mycobacterium tuberculosis Affect gltA1, lprQ, and rpsO Expression |
| title_sort | two new dihydrosphingosine analogs against mycobacterium tuberculosis affect glta1 lprq and rpso expression |
| topic | QR Microbiología |
| url | http://eprints.uanl.mx/23508/1/23508.pdf |
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