Starch-mediated synthesis of mono- and bimetallic silver/gold nanoparticles as antimicrobial and anticancer agents
Background and aim: Bimetallic silver/gold nanosystems are expected to significantly improve therapeutic efficacy compared to their monometallic counterparts by maintaining the general biocompatibility of gold nanoparticles (AuNPs) while, at the same time, decreasing the relatively high toxicity of...
| Autores principales: | , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
Dove Medical Press
2019
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| Materias: | |
| Acceso en línea: | http://eprints.uanl.mx/23470/1/23470.pdf |
| _version_ | 1824416895921553408 |
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| author | Lomelí Marroquín, Diana Medina Cruz, David Nieto Argüello, Alfonso Vernet Crua, Ada Chen, Junjiang Torres Castro, Alejandro Webster, Thomas J Cholula Díaz, Jorge L |
| author_facet | Lomelí Marroquín, Diana Medina Cruz, David Nieto Argüello, Alfonso Vernet Crua, Ada Chen, Junjiang Torres Castro, Alejandro Webster, Thomas J Cholula Díaz, Jorge L |
| author_sort | Lomelí Marroquín, Diana |
| collection | Repositorio Institucional |
| description | Background and aim: Bimetallic silver/gold nanosystems are expected to significantly improve therapeutic efficacy compared to their monometallic counterparts by maintaining the general biocompatibility of gold nanoparticles (AuNPs) while, at the same time, decreasing the relatively high toxicity of silver nanoparticles (AgNPs) toward healthy human cells. Thus, the aim of this research was to establish a highly reproducible one-pot green synthesis of colloidal AuNPs and bimetallic Ag/Au alloy nanoparticles (NPs; Ag/AuNPs) using starch as reducing and capping agent. Methods: The optical properties, high reproducibility, stability and particle size distribution of the colloidal NPs were analyzed by ultraviolet (UV)–visible spectroscopy, dynamic light scattering (DLS) and ζ-potential. The presence of starch as capping agent was determined by Fourier transform infrared (FT-IR) spectroscopy. The structural properties were studied by X-ray diffraction (XRD). Transmission electron microscopy (TEM) imaging was done to determine the morphology and size of the nanostructures. The chemical composition of the nanomaterials was determined by energy-dispersive X-ray spectroscopy (EDS) and inductively coupled plasma mass spectrometry (ICP-MS) analysis. To further study the biomedical applications of the synthesized nanostructures, antibacterial studies against multidrug-resistant (MDR) Escherichia coli and methicillin-resistant Staphylococcus aureus (MRSA) were conducted. In addition, the NPs were added to the growth media of human dermal fibroblast (HDF) and human melanoma cells to show their cytocompatibility and cytotoxicity, respectively, over a 3-day experiment. Results: UV–visible spectroscopy confirmed the highly reproducible green synthesis of colloidal AuNPs and Ag/AuNPs. The NPs showed a face-centered cubic crystal structure and an icosahedral shape with mean particle sizes of 28.5 and 9.7 nm for AuNPs and Ag/AuNPs, respectively. The antibacterial studies of the NPs against antibiotic-resistant bacterial strains presented a dose-dependent antimicrobial behavior. Furthermore, the NPs showed cytocompatibility towards HDF, but a dose-dependent anticancer effect was found when human melanoma cells were grown in presence of different NP concentrations for 72 hours. Conclusion: In this study, mono- and bimetallic NPs were synthesized for the first time using a highly reproducible, environmentally friendly, cost-effective and quick method and were successfully characterized and tested for several anti-infection and anticancer biomedical applications. |
| format | Article |
| id | eprints-23470 |
| institution | UANL |
| language | English |
| publishDate | 2019 |
| publisher | Dove Medical Press |
| record_format | eprints |
| spelling | eprints-234702022-09-02T18:32:29Z http://eprints.uanl.mx/23470/ Starch-mediated synthesis of mono- and bimetallic silver/gold nanoparticles as antimicrobial and anticancer agents Lomelí Marroquín, Diana Medina Cruz, David Nieto Argüello, Alfonso Vernet Crua, Ada Chen, Junjiang Torres Castro, Alejandro Webster, Thomas J Cholula Díaz, Jorge L QD Química Background and aim: Bimetallic silver/gold nanosystems are expected to significantly improve therapeutic efficacy compared to their monometallic counterparts by maintaining the general biocompatibility of gold nanoparticles (AuNPs) while, at the same time, decreasing the relatively high toxicity of silver nanoparticles (AgNPs) toward healthy human cells. Thus, the aim of this research was to establish a highly reproducible one-pot green synthesis of colloidal AuNPs and bimetallic Ag/Au alloy nanoparticles (NPs; Ag/AuNPs) using starch as reducing and capping agent. Methods: The optical properties, high reproducibility, stability and particle size distribution of the colloidal NPs were analyzed by ultraviolet (UV)–visible spectroscopy, dynamic light scattering (DLS) and ζ-potential. The presence of starch as capping agent was determined by Fourier transform infrared (FT-IR) spectroscopy. The structural properties were studied by X-ray diffraction (XRD). Transmission electron microscopy (TEM) imaging was done to determine the morphology and size of the nanostructures. The chemical composition of the nanomaterials was determined by energy-dispersive X-ray spectroscopy (EDS) and inductively coupled plasma mass spectrometry (ICP-MS) analysis. To further study the biomedical applications of the synthesized nanostructures, antibacterial studies against multidrug-resistant (MDR) Escherichia coli and methicillin-resistant Staphylococcus aureus (MRSA) were conducted. In addition, the NPs were added to the growth media of human dermal fibroblast (HDF) and human melanoma cells to show their cytocompatibility and cytotoxicity, respectively, over a 3-day experiment. Results: UV–visible spectroscopy confirmed the highly reproducible green synthesis of colloidal AuNPs and Ag/AuNPs. The NPs showed a face-centered cubic crystal structure and an icosahedral shape with mean particle sizes of 28.5 and 9.7 nm for AuNPs and Ag/AuNPs, respectively. The antibacterial studies of the NPs against antibiotic-resistant bacterial strains presented a dose-dependent antimicrobial behavior. Furthermore, the NPs showed cytocompatibility towards HDF, but a dose-dependent anticancer effect was found when human melanoma cells were grown in presence of different NP concentrations for 72 hours. Conclusion: In this study, mono- and bimetallic NPs were synthesized for the first time using a highly reproducible, environmentally friendly, cost-effective and quick method and were successfully characterized and tested for several anti-infection and anticancer biomedical applications. Dove Medical Press 2019 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/23470/1/23470.pdf http://eprints.uanl.mx/23470/1.haspreviewThumbnailVersion/23470.pdf Lomelí Marroquín, Diana y Medina Cruz, David y Nieto Argüello, Alfonso y Vernet Crua, Ada y Chen, Junjiang y Torres Castro, Alejandro y Webster, Thomas J y Cholula Díaz, Jorge L (2019) Starch-mediated synthesis of mono- and bimetallic silver/gold nanoparticles as antimicrobial and anticancer agents. International Journal of Nanomedicine, 14. pp. 2171-2190. ISSN 1178-2013 http://doi.org/10.2147/IJN.S192757 doi:10.2147/IJN.S192757 |
| spellingShingle | QD Química Lomelí Marroquín, Diana Medina Cruz, David Nieto Argüello, Alfonso Vernet Crua, Ada Chen, Junjiang Torres Castro, Alejandro Webster, Thomas J Cholula Díaz, Jorge L Starch-mediated synthesis of mono- and bimetallic silver/gold nanoparticles as antimicrobial and anticancer agents |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Starch-mediated synthesis of mono- and bimetallic silver/gold nanoparticles as antimicrobial and anticancer agents |
| title_full | Starch-mediated synthesis of mono- and bimetallic silver/gold nanoparticles as antimicrobial and anticancer agents |
| title_fullStr | Starch-mediated synthesis of mono- and bimetallic silver/gold nanoparticles as antimicrobial and anticancer agents |
| title_full_unstemmed | Starch-mediated synthesis of mono- and bimetallic silver/gold nanoparticles as antimicrobial and anticancer agents |
| title_short | Starch-mediated synthesis of mono- and bimetallic silver/gold nanoparticles as antimicrobial and anticancer agents |
| title_sort | starch mediated synthesis of mono and bimetallic silver gold nanoparticles as antimicrobial and anticancer agents |
| topic | QD Química |
| url | http://eprints.uanl.mx/23470/1/23470.pdf |
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