A bioactive cartilage graft of IGF1-transduced adipose mesenchymal stem cells embedded in an alginate/bovine cartilage matrix tridimensional scaffold

Articular cartilage injuries remain as a therapeutic challenge due to the limited regeneration potential of this tissue. Cartilage engineering grafts combining chondrogenic cells, scaffold materials, and microenvironmental factors are emerging as promissory alternatives. The design of an adequate sc...

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Autores principales: Moncada Saucedo, Nidia Karina, Marino Martínez, Iván Alberto, Lara Arias, Jorge, Romero Díaz, Víktor J., Camacho Morales, Alberto, Valdés Franco, José A., Pérez Silos, Vanessa, García Ruíz, Alejandro, Lin, Hang, Tuan, Rocky S., Ramos Payán, Rosalío, Lara Banda, María del Refugio, Ortiz López, Rocío, Rojas Martínez, Augusto, Fuentes Mera, Lizeth
Formato: Artículo
Lenguaje:inglés
Publicado: Hindawi Publishing Corporation 2019
Materias:
Acceso en línea:http://eprints.uanl.mx/23332/1/23332.pdf
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author Moncada Saucedo, Nidia Karina
Marino Martínez, Iván Alberto
Lara Arias, Jorge
Romero Díaz, Víktor J.
Camacho Morales, Alberto
Valdés Franco, José A.
Pérez Silos, Vanessa
García Ruíz, Alejandro
Lin, Hang
Tuan, Rocky S.
Ramos Payán, Rosalío
Lara Banda, María del Refugio
Ortiz López, Rocío
Rojas Martínez, Augusto
Fuentes Mera, Lizeth
author_facet Moncada Saucedo, Nidia Karina
Marino Martínez, Iván Alberto
Lara Arias, Jorge
Romero Díaz, Víktor J.
Camacho Morales, Alberto
Valdés Franco, José A.
Pérez Silos, Vanessa
García Ruíz, Alejandro
Lin, Hang
Tuan, Rocky S.
Ramos Payán, Rosalío
Lara Banda, María del Refugio
Ortiz López, Rocío
Rojas Martínez, Augusto
Fuentes Mera, Lizeth
author_sort Moncada Saucedo, Nidia Karina
collection Repositorio Institucional
description Articular cartilage injuries remain as a therapeutic challenge due to the limited regeneration potential of this tissue. Cartilage engineering grafts combining chondrogenic cells, scaffold materials, and microenvironmental factors are emerging as promissory alternatives. The design of an adequate scaffold resembling the physicochemical features of natural cartilage and able to support chondrogenesis in the implants is a crucial topic to solve. This study reports the development of an implant constructed with IGF1-transduced adipose-derived mesenchymal stem cells (immunophenotypes: CD105+, CD90+, CD73+, CD14-, and CD34-) embedded in a scaffold composed of a mix of alginate/milled bovine decellularized knee material which was cultivated in vitro for 28 days (3CI). Histological analyses demonstrated the distribution into isogenous groups of chondrocytes surrounded by a de novo dense extracellular matrix with balanced proportions of collagens II and I and high amounts of sulfated proteoglycans which also evidenced adequate cell proliferation and differentiation. This graft also shoved mechanical properties resembling the natural knee cartilage. A modified Bern/O’Driscoll scale showed that the 3CI implants had a significantly higher score than the 2CI implants lacking cells transduced with IGF1 (16/18 vs. 14/18), representing high-quality engineering cartilage suitable for in vivo tests. This study suggests that this graft resembles several features of typical hyaline cartilage and will be promissory for preclinical studies for cartilage regeneration.
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spelling eprints-233322024-03-05T15:31:27Z http://eprints.uanl.mx/23332/ A bioactive cartilage graft of IGF1-transduced adipose mesenchymal stem cells embedded in an alginate/bovine cartilage matrix tridimensional scaffold Moncada Saucedo, Nidia Karina Marino Martínez, Iván Alberto Lara Arias, Jorge Romero Díaz, Víktor J. Camacho Morales, Alberto Valdés Franco, José A. Pérez Silos, Vanessa García Ruíz, Alejandro Lin, Hang Tuan, Rocky S. Ramos Payán, Rosalío Lara Banda, María del Refugio Ortiz López, Rocío Rojas Martínez, Augusto Fuentes Mera, Lizeth QR Microbiología Articular cartilage injuries remain as a therapeutic challenge due to the limited regeneration potential of this tissue. Cartilage engineering grafts combining chondrogenic cells, scaffold materials, and microenvironmental factors are emerging as promissory alternatives. The design of an adequate scaffold resembling the physicochemical features of natural cartilage and able to support chondrogenesis in the implants is a crucial topic to solve. This study reports the development of an implant constructed with IGF1-transduced adipose-derived mesenchymal stem cells (immunophenotypes: CD105+, CD90+, CD73+, CD14-, and CD34-) embedded in a scaffold composed of a mix of alginate/milled bovine decellularized knee material which was cultivated in vitro for 28 days (3CI). Histological analyses demonstrated the distribution into isogenous groups of chondrocytes surrounded by a de novo dense extracellular matrix with balanced proportions of collagens II and I and high amounts of sulfated proteoglycans which also evidenced adequate cell proliferation and differentiation. This graft also shoved mechanical properties resembling the natural knee cartilage. A modified Bern/O’Driscoll scale showed that the 3CI implants had a significantly higher score than the 2CI implants lacking cells transduced with IGF1 (16/18 vs. 14/18), representing high-quality engineering cartilage suitable for in vivo tests. This study suggests that this graft resembles several features of typical hyaline cartilage and will be promissory for preclinical studies for cartilage regeneration. Hindawi Publishing Corporation 2019 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/23332/1/23332.pdf http://eprints.uanl.mx/23332/1.haspreviewThumbnailVersion/23332.pdf Moncada Saucedo, Nidia Karina y Marino Martínez, Iván Alberto y Lara Arias, Jorge y Romero Díaz, Víktor J. y Camacho Morales, Alberto y Valdés Franco, José A. y Pérez Silos, Vanessa y García Ruíz, Alejandro y Lin, Hang y Tuan, Rocky S. y Ramos Payán, Rosalío y Lara Banda, María del Refugio y Ortiz López, Rocío y Rojas Martínez, Augusto y Fuentes Mera, Lizeth (2019) A bioactive cartilage graft of IGF1-transduced adipose mesenchymal stem cells embedded in an alginate/bovine cartilage matrix tridimensional scaffold. Stem Cells International, 2019. pp. 1-15. ISSN 1687-966X http://doi.org/10.1155/2019/9792369 doi:10.1155/2019/9792369
spellingShingle QR Microbiología
Moncada Saucedo, Nidia Karina
Marino Martínez, Iván Alberto
Lara Arias, Jorge
Romero Díaz, Víktor J.
Camacho Morales, Alberto
Valdés Franco, José A.
Pérez Silos, Vanessa
García Ruíz, Alejandro
Lin, Hang
Tuan, Rocky S.
Ramos Payán, Rosalío
Lara Banda, María del Refugio
Ortiz López, Rocío
Rojas Martínez, Augusto
Fuentes Mera, Lizeth
A bioactive cartilage graft of IGF1-transduced adipose mesenchymal stem cells embedded in an alginate/bovine cartilage matrix tridimensional scaffold
thumbnail https://rediab.uanl.mx/themes/sandal5/images/online.png
title A bioactive cartilage graft of IGF1-transduced adipose mesenchymal stem cells embedded in an alginate/bovine cartilage matrix tridimensional scaffold
title_full A bioactive cartilage graft of IGF1-transduced adipose mesenchymal stem cells embedded in an alginate/bovine cartilage matrix tridimensional scaffold
title_fullStr A bioactive cartilage graft of IGF1-transduced adipose mesenchymal stem cells embedded in an alginate/bovine cartilage matrix tridimensional scaffold
title_full_unstemmed A bioactive cartilage graft of IGF1-transduced adipose mesenchymal stem cells embedded in an alginate/bovine cartilage matrix tridimensional scaffold
title_short A bioactive cartilage graft of IGF1-transduced adipose mesenchymal stem cells embedded in an alginate/bovine cartilage matrix tridimensional scaffold
title_sort bioactive cartilage graft of igf1 transduced adipose mesenchymal stem cells embedded in an alginate bovine cartilage matrix tridimensional scaffold
topic QR Microbiología
url http://eprints.uanl.mx/23332/1/23332.pdf
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