Biofilm formation and molecular analysis of intercellular adhesion gene cluster (icaABCD) among Staphylococcus aureus strains isolated from children with adenoiditis

Background and Objectives: It is well known that Staphylococcus aureus biofilm plays an important role in adenoiditis and biofilm resistance frequently results in failure of therapy. The goal of this study was to evaluate the biofilm production of S. aureus isolates obtained from adenoid specimens a...

Descripción completa

Detalles Bibliográficos
Autores principales: Ghaioumy, Rasoul, Tabatabaeifar, Fatemehalsadat, Mozafarinia, Karamat, Arabi Mianroodi, Aliasghar, Isaei, Elham, Morones Ramírez, José Rubén, Agha Kuchak Afshari, Setareh, Kalantar Neyestanaki, Davood
Formato: Artículo
Lenguaje:inglés
Publicado: Tehran University of Medical Sciences 2021
Materias:
Acceso en línea:http://eprints.uanl.mx/23318/1/23318.pdf
_version_ 1824416839181008896
author Ghaioumy, Rasoul
Tabatabaeifar, Fatemehalsadat
Mozafarinia, Karamat
Arabi Mianroodi, Aliasghar
Isaei, Elham
Morones Ramírez, José Rubén
Agha Kuchak Afshari, Setareh
Kalantar Neyestanaki, Davood
author_facet Ghaioumy, Rasoul
Tabatabaeifar, Fatemehalsadat
Mozafarinia, Karamat
Arabi Mianroodi, Aliasghar
Isaei, Elham
Morones Ramírez, José Rubén
Agha Kuchak Afshari, Setareh
Kalantar Neyestanaki, Davood
author_sort Ghaioumy, Rasoul
collection Repositorio Institucional
description Background and Objectives: It is well known that Staphylococcus aureus biofilm plays an important role in adenoiditis and biofilm resistance frequently results in failure of therapy. The goal of this study was to evaluate the biofilm production of S. aureus isolates obtained from adenoid specimens and assess the relationship between biofilm formation ability and ica operon genes. Materials and Methods: A total of 112 adenoid samples were obtained from patients under 15 years old with adenoid hypertrophy. All S. aureus isolates were initially identified by standard microbiological tests and amplification of nuc by polymerase chain reaction (PCR) technique. Biofilm formation of S. aureus isolates was evaluated and icaADBC genes were detected by PCR technique. Results: There were 46 isolates (41%) identified as S. aureus. The ability to produce biofilm was detected among total S. aureus isolates. Molecular study of ica operon revealed that 2 (6.3%) and 19 (59.4%) isolates carried icaA and icaD, respectively. The prevalence of icaA + icaD was seen among 11 (34.4%) S. aureus isolates, while icaC and icaB were not detected. Conclusion: Our findings indicated that icaABCD operon are associated with biofilm formation in S. aureus isolates, however the absence of these genes may not necessarily exclude this property.
format Article
id eprints-23318
institution UANL
language English
publishDate 2021
publisher Tehran University of Medical Sciences
record_format eprints
spelling eprints-233182022-05-30T21:15:34Z http://eprints.uanl.mx/23318/ Biofilm formation and molecular analysis of intercellular adhesion gene cluster (icaABCD) among Staphylococcus aureus strains isolated from children with adenoiditis Ghaioumy, Rasoul Tabatabaeifar, Fatemehalsadat Mozafarinia, Karamat Arabi Mianroodi, Aliasghar Isaei, Elham Morones Ramírez, José Rubén Agha Kuchak Afshari, Setareh Kalantar Neyestanaki, Davood QR Microbiología Background and Objectives: It is well known that Staphylococcus aureus biofilm plays an important role in adenoiditis and biofilm resistance frequently results in failure of therapy. The goal of this study was to evaluate the biofilm production of S. aureus isolates obtained from adenoid specimens and assess the relationship between biofilm formation ability and ica operon genes. Materials and Methods: A total of 112 adenoid samples were obtained from patients under 15 years old with adenoid hypertrophy. All S. aureus isolates were initially identified by standard microbiological tests and amplification of nuc by polymerase chain reaction (PCR) technique. Biofilm formation of S. aureus isolates was evaluated and icaADBC genes were detected by PCR technique. Results: There were 46 isolates (41%) identified as S. aureus. The ability to produce biofilm was detected among total S. aureus isolates. Molecular study of ica operon revealed that 2 (6.3%) and 19 (59.4%) isolates carried icaA and icaD, respectively. The prevalence of icaA + icaD was seen among 11 (34.4%) S. aureus isolates, while icaC and icaB were not detected. Conclusion: Our findings indicated that icaABCD operon are associated with biofilm formation in S. aureus isolates, however the absence of these genes may not necessarily exclude this property. Tehran University of Medical Sciences 2021 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/23318/1/23318.pdf http://eprints.uanl.mx/23318/1.haspreviewThumbnailVersion/23318.pdf Ghaioumy, Rasoul y Tabatabaeifar, Fatemehalsadat y Mozafarinia, Karamat y Arabi Mianroodi, Aliasghar y Isaei, Elham y Morones Ramírez, José Rubén y Agha Kuchak Afshari, Setareh y Kalantar Neyestanaki, Davood (2021) Biofilm formation and molecular analysis of intercellular adhesion gene cluster (icaABCD) among Staphylococcus aureus strains isolated from children with adenoiditis. Iranian Journal of Microbiology, 13 (4). pp. 458-463. ISSN 2008-3289 http://doi.org/10.18502/ijm.v13i4.6969 doi:10.18502/ijm.v13i4.6969
spellingShingle QR Microbiología
Ghaioumy, Rasoul
Tabatabaeifar, Fatemehalsadat
Mozafarinia, Karamat
Arabi Mianroodi, Aliasghar
Isaei, Elham
Morones Ramírez, José Rubén
Agha Kuchak Afshari, Setareh
Kalantar Neyestanaki, Davood
Biofilm formation and molecular analysis of intercellular adhesion gene cluster (icaABCD) among Staphylococcus aureus strains isolated from children with adenoiditis
thumbnail https://rediab.uanl.mx/themes/sandal5/images/online.png
title Biofilm formation and molecular analysis of intercellular adhesion gene cluster (icaABCD) among Staphylococcus aureus strains isolated from children with adenoiditis
title_full Biofilm formation and molecular analysis of intercellular adhesion gene cluster (icaABCD) among Staphylococcus aureus strains isolated from children with adenoiditis
title_fullStr Biofilm formation and molecular analysis of intercellular adhesion gene cluster (icaABCD) among Staphylococcus aureus strains isolated from children with adenoiditis
title_full_unstemmed Biofilm formation and molecular analysis of intercellular adhesion gene cluster (icaABCD) among Staphylococcus aureus strains isolated from children with adenoiditis
title_short Biofilm formation and molecular analysis of intercellular adhesion gene cluster (icaABCD) among Staphylococcus aureus strains isolated from children with adenoiditis
title_sort biofilm formation and molecular analysis of intercellular adhesion gene cluster icaabcd among staphylococcus aureus strains isolated from children with adenoiditis
topic QR Microbiología
url http://eprints.uanl.mx/23318/1/23318.pdf
work_keys_str_mv AT ghaioumyrasoul biofilmformationandmolecularanalysisofintercellularadhesiongeneclustericaabcdamongstaphylococcusaureusstrainsisolatedfromchildrenwithadenoiditis
AT tabatabaeifarfatemehalsadat biofilmformationandmolecularanalysisofintercellularadhesiongeneclustericaabcdamongstaphylococcusaureusstrainsisolatedfromchildrenwithadenoiditis
AT mozafariniakaramat biofilmformationandmolecularanalysisofintercellularadhesiongeneclustericaabcdamongstaphylococcusaureusstrainsisolatedfromchildrenwithadenoiditis
AT arabimianroodialiasghar biofilmformationandmolecularanalysisofintercellularadhesiongeneclustericaabcdamongstaphylococcusaureusstrainsisolatedfromchildrenwithadenoiditis
AT isaeielham biofilmformationandmolecularanalysisofintercellularadhesiongeneclustericaabcdamongstaphylococcusaureusstrainsisolatedfromchildrenwithadenoiditis
AT moronesramirezjoseruben biofilmformationandmolecularanalysisofintercellularadhesiongeneclustericaabcdamongstaphylococcusaureusstrainsisolatedfromchildrenwithadenoiditis
AT aghakuchakafsharisetareh biofilmformationandmolecularanalysisofintercellularadhesiongeneclustericaabcdamongstaphylococcusaureusstrainsisolatedfromchildrenwithadenoiditis
AT kalantarneyestanakidavood biofilmformationandmolecularanalysisofintercellularadhesiongeneclustericaabcdamongstaphylococcusaureusstrainsisolatedfromchildrenwithadenoiditis