Biofilm formation and molecular analysis of intercellular adhesion gene cluster (icaABCD) among Staphylococcus aureus strains isolated from children with adenoiditis
Background and Objectives: It is well known that Staphylococcus aureus biofilm plays an important role in adenoiditis and biofilm resistance frequently results in failure of therapy. The goal of this study was to evaluate the biofilm production of S. aureus isolates obtained from adenoid specimens a...
| Autores principales: | , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | inglés |
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Tehran University of Medical Sciences
2021
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| Acceso en línea: | http://eprints.uanl.mx/23318/1/23318.pdf |
| _version_ | 1824416839181008896 |
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| author | Ghaioumy, Rasoul Tabatabaeifar, Fatemehalsadat Mozafarinia, Karamat Arabi Mianroodi, Aliasghar Isaei, Elham Morones Ramírez, José Rubén Agha Kuchak Afshari, Setareh Kalantar Neyestanaki, Davood |
| author_facet | Ghaioumy, Rasoul Tabatabaeifar, Fatemehalsadat Mozafarinia, Karamat Arabi Mianroodi, Aliasghar Isaei, Elham Morones Ramírez, José Rubén Agha Kuchak Afshari, Setareh Kalantar Neyestanaki, Davood |
| author_sort | Ghaioumy, Rasoul |
| collection | Repositorio Institucional |
| description | Background and Objectives: It is well known that Staphylococcus aureus biofilm plays an important role in adenoiditis and biofilm resistance frequently results in failure of therapy. The goal of this study was to evaluate the biofilm production of S. aureus isolates obtained from adenoid specimens and assess the relationship between biofilm formation ability and ica operon genes. Materials and Methods: A total of 112 adenoid samples were obtained from patients under 15 years old with adenoid hypertrophy. All S. aureus isolates were initially identified by standard microbiological tests and amplification of nuc by polymerase chain reaction (PCR) technique. Biofilm formation of S. aureus isolates was evaluated and icaADBC genes were detected by PCR technique. Results: There were 46 isolates (41%) identified as S. aureus. The ability to produce biofilm was detected among total S. aureus isolates. Molecular study of ica operon revealed that 2 (6.3%) and 19 (59.4%) isolates carried icaA and icaD, respectively. The prevalence of icaA + icaD was seen among 11 (34.4%) S. aureus isolates, while icaC and icaB were not detected. Conclusion: Our findings indicated that icaABCD operon are associated with biofilm formation in S. aureus isolates, however the absence of these genes may not necessarily exclude this property. |
| format | Article |
| id | eprints-23318 |
| institution | UANL |
| language | English |
| publishDate | 2021 |
| publisher | Tehran University of Medical Sciences |
| record_format | eprints |
| spelling | eprints-233182022-05-30T21:15:34Z http://eprints.uanl.mx/23318/ Biofilm formation and molecular analysis of intercellular adhesion gene cluster (icaABCD) among Staphylococcus aureus strains isolated from children with adenoiditis Ghaioumy, Rasoul Tabatabaeifar, Fatemehalsadat Mozafarinia, Karamat Arabi Mianroodi, Aliasghar Isaei, Elham Morones Ramírez, José Rubén Agha Kuchak Afshari, Setareh Kalantar Neyestanaki, Davood QR Microbiología Background and Objectives: It is well known that Staphylococcus aureus biofilm plays an important role in adenoiditis and biofilm resistance frequently results in failure of therapy. The goal of this study was to evaluate the biofilm production of S. aureus isolates obtained from adenoid specimens and assess the relationship between biofilm formation ability and ica operon genes. Materials and Methods: A total of 112 adenoid samples were obtained from patients under 15 years old with adenoid hypertrophy. All S. aureus isolates were initially identified by standard microbiological tests and amplification of nuc by polymerase chain reaction (PCR) technique. Biofilm formation of S. aureus isolates was evaluated and icaADBC genes were detected by PCR technique. Results: There were 46 isolates (41%) identified as S. aureus. The ability to produce biofilm was detected among total S. aureus isolates. Molecular study of ica operon revealed that 2 (6.3%) and 19 (59.4%) isolates carried icaA and icaD, respectively. The prevalence of icaA + icaD was seen among 11 (34.4%) S. aureus isolates, while icaC and icaB were not detected. Conclusion: Our findings indicated that icaABCD operon are associated with biofilm formation in S. aureus isolates, however the absence of these genes may not necessarily exclude this property. Tehran University of Medical Sciences 2021 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/23318/1/23318.pdf http://eprints.uanl.mx/23318/1.haspreviewThumbnailVersion/23318.pdf Ghaioumy, Rasoul y Tabatabaeifar, Fatemehalsadat y Mozafarinia, Karamat y Arabi Mianroodi, Aliasghar y Isaei, Elham y Morones Ramírez, José Rubén y Agha Kuchak Afshari, Setareh y Kalantar Neyestanaki, Davood (2021) Biofilm formation and molecular analysis of intercellular adhesion gene cluster (icaABCD) among Staphylococcus aureus strains isolated from children with adenoiditis. Iranian Journal of Microbiology, 13 (4). pp. 458-463. ISSN 2008-3289 http://doi.org/10.18502/ijm.v13i4.6969 doi:10.18502/ijm.v13i4.6969 |
| spellingShingle | QR Microbiología Ghaioumy, Rasoul Tabatabaeifar, Fatemehalsadat Mozafarinia, Karamat Arabi Mianroodi, Aliasghar Isaei, Elham Morones Ramírez, José Rubén Agha Kuchak Afshari, Setareh Kalantar Neyestanaki, Davood Biofilm formation and molecular analysis of intercellular adhesion gene cluster (icaABCD) among Staphylococcus aureus strains isolated from children with adenoiditis |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Biofilm formation and molecular analysis of intercellular adhesion gene cluster (icaABCD) among Staphylococcus aureus strains isolated from children with adenoiditis |
| title_full | Biofilm formation and molecular analysis of intercellular adhesion gene cluster (icaABCD) among Staphylococcus aureus strains isolated from children with adenoiditis |
| title_fullStr | Biofilm formation and molecular analysis of intercellular adhesion gene cluster (icaABCD) among Staphylococcus aureus strains isolated from children with adenoiditis |
| title_full_unstemmed | Biofilm formation and molecular analysis of intercellular adhesion gene cluster (icaABCD) among Staphylococcus aureus strains isolated from children with adenoiditis |
| title_short | Biofilm formation and molecular analysis of intercellular adhesion gene cluster (icaABCD) among Staphylococcus aureus strains isolated from children with adenoiditis |
| title_sort | biofilm formation and molecular analysis of intercellular adhesion gene cluster icaabcd among staphylococcus aureus strains isolated from children with adenoiditis |
| topic | QR Microbiología |
| url | http://eprints.uanl.mx/23318/1/23318.pdf |
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